2-nitro-p-toluidine

Endpoint summary

Description of key information

From the available results; the chemical 2-nitro-p-toluidine, is expected to exhibit acute toxic effects in the category 4 (based upon the LD50 and LC50 values mentioned in the CLP regulation) by the oral, inhalation and dermal route. 

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Justification for type of information:

QSAR prediction: migrated from IUCLID 5.6

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

Prediction is done using QSAR Toolbox version 2.3.

GLP compliance:

no

Species:

rat

Strain:

not specified

Sex:

not specified

Route of administration:

oral: unspecified

Vehicle:

not specified

Control animals:

not specified

Sex:

not specified

Dose descriptor:

LD50

Effect level:

590.944 mg/kg bw

Based on:

test mat.

Remarks on result:

other: other details not available

The prediction was based on dataset comprised from the following descriptors: LD50
Estimation method: Taking average value from the 5 nearest neighbours
Domain logical expression:Result: In Domain

((("a" and "b" ) and "c" ) and ("d" and "e" ) )

Domain logical expression index: "a"

Similarity boundary:Target: c1(N)c(N(=O)=O)cc(C)cc1
Threshold=50%,
Dice(Atom pairs)

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as High (Class III) by Toxic hazard classification by Cramer (with extension)

Domain logical expression index: "c"

Similarity boundary:Target: c1(N)c(N(=O)=O)cc(C)cc1
Threshold=60%,
Dice(Atom pairs)

Domain logical expression index: "d"

Parametric boundary:The target chemical should have a value of log Kow which is >= 1.91

Domain logical expression index: "e"

Parametric boundary:The target chemical should have a value of log Kow which is <= 3.1

Interpretation of results:

Toxicity Category IV

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The acute oral median lethal dose (LD50) of 2-nitro-p-toluidine in rat (Rattus norvegicus) was found to be 590.9436 mg/kg of body weight. Acute toxicity of 2-nitro-p-toluidine to rat by the oral route indicates that the substance exhibits acute toxicity category IV by the oral route.

Executive summary:

The acute oral median lethal dose (LD50) of 2-nitro-p-toluidine in rat (Rattus norvegicus) was found to be 590.9436 mg/kg of body weight. Acute toxicity of 2-nitro-p-toluidine to rat by the oral route indicates that the substance shall exhibit acute toxicity in category IV by the oral route.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed

Dose descriptor:

LD50

Value:

590.944 mg/kg bw

Quality of whole database:

Weight of evidence approach has been used using predictions from model considered reliable by OECD and authoritattive Swedish chemical agency database.

Acute toxicity: via inhalation route

Link to relevant study records

Reference

Endpoint:

acute toxicity: inhalation

Type of information:

(Q)SAR

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Justification for type of information:

QSAR prediction: migrated from IUCLID 5.6

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

no

Test type:

other: Acute Rodent Inhalation Toxicity Test;standard acute method

Species:

rat

Strain:

other: Wistar;CnR-CD;Sprague-Dawley;Long-Evans;no data

Sex:

not specified

Route of administration:

inhalation

Type of inhalation exposure:

other: Inhalation: Vapor

Vehicle:

not specified

Duration of exposure:

4 h

Control animals:

not specified

Sex:

not specified

Dose descriptor:

LC50

Effect level:

19.317 other: mg/L

Based on:

test mat.

Exp. duration:

4 h

The prediction was based on dataset comprised from the following descriptors: LC50
Estimation method: Taking average value from the 5 nearest neighbours
Domain logical expression:Result: In Domain

("a" and ("b" and "c" and "d" and "e" ) )

Domain logical expression index: "a"

Similarity boundary:Target: c1(N)c(N(=O)=O)cc(C)cc1
Threshold=50%,
Dice(Atom pairs)

Domain logical expression index: "b"

Parametric boundary:The target chemical should have a value of log Kow which is >= 2.15

Domain logical expression index: "c"

Parametric boundary:The target chemical should have a value of log Kow which is <= 3.2

Domain logical expression index: "d"

Parametric boundary:The target chemical should have a value of Molecular weight which is >= 137 Da

Domain logical expression index: "e"

Parametric boundary:The target chemical should have a value of Molecular weight which is <= 168 Da

Interpretation of results:

Toxicity Category IV

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The 4 hr acute inhalation median lethal concentration (LC50) of 2-nitro-p-toluidine in rat was estimated to be 19.31692 mg/L. Acute inhalation toxicity of 2-nitro-p-toluidine to rat by inhalative route indicates that the substance exhibits acute toxicity Category IV type by the inhalative route.

Executive summary:

The 4 hr acute inhalation median lethal concentration (LC50) of 2-nitro-p-toluidine in rat was estimated to be 19.31692 mg/L. Acute inhalation toxicity of 2-nitro-p-toluidine to rat by inhalative route indicates that the substance exhibits acute toxicity Category IV type by the inhalative route.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed

Dose descriptor:

LC50

Value:

19.316

Quality of whole database:

Model considered reliable by OECD

Acute toxicity: via dermal route

Link to relevant study records

Reference

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

Kemi-Riskline database

GLP compliance:

not specified

Test type:

standard acute method

Species:

guinea pig

Strain:

not specified

Sex:

not specified

Type of coverage:

not specified

Vehicle:

not specified

Control animals:

not specified

Sex:

not specified

Dose descriptor:

LD50

Effect level:

> 1 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: other details not available

Interpretation of results:

Toxicity Category IV

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Acute dermal toxicity value of 2-nitro-p-toluidine to guinea pig is found to be LD50: > 1000 mg/kg body weight. Thus, it is assumed that the chemical could potentially exhibit dermal toxicity in acute catergory 4 as per the CLP regulation guidelines.

Executive summary:

Acute dermal toxicity value of 2-nitro-p-toluidine to guinea pig is found to be LD50: > 1000 mg/kg body weight. Thus, it is assumed that the chemical could potentially exhibit dermal toxicity in acute catergory 4 as per the CLP regulation guidelines.

Endpoint conclusion

Endpoint conclusion:

adverse effect observed

Dose descriptor:

LD50

Value:

1 000 mg/kg bw

Quality of whole database:

Source of information is an authoritative database.

Additional information

The summary of the weight of evidence study of the acute toxicity by the oral route is presented below

S. No	End point	Value in mg/kg bw	Species
1	LC50	590.9436	Rattus norvegicus
2	LC50	175.3525	Mouse (Mus musculus)
3	LC50	1132.849	Mouse (Peromyscus maniculatus)
4	LC50	1903	Rat (Rattus norvegicus)
5	LC50
6	LC50

Justification for selection of acute toxicity – oral endpoint
Model considered reliable by OECD

Justification for selection of acute toxicity – inhalation endpoint
Model considered reliable by OECD and prediction suggests that the chemical 2-nitro-p-toluidine shall exhibit acute toxicity by the inhalation route in the category 4 sicen the LC50 value of 19.316 mg/L has been obtained

Justification for selection of acute toxicity – dermal endpoint
Acute dermal toxicity value of 2-nitro-p-toluidine to guinea pig is found to be LD50: > 1000 mg/kg body weight. Thus, it is assumed that the chemical could potentially exhibit dermal toxicity in acute catergory 4 as per the CLP regulation guidelines.

Justification for classification or non-classification

From the available results; the chemical 2-nitro-p-toluidine, is expected to exhibit acute toxic effects in the category 4 (based upon the LD50 and LC50 values mentioned in the CLP regulation) by the oral, inhalation and dermal route.