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Diss Factsheets

Ecotoxicological information

Toxicity to terrestrial plants

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- Concentrations: Low mid and high concentration was analysed for quantity of test chemical in exposed concentrations.
Range of average analyzed content of active ingredient (% of nominal)
Plant species Test conc. % recovery
Onion 15, 135 and 1215 81.630 - 93.599
Tomato 5, 45 and 405 92.714 - 96.095
Corn and cabbage 32, 200 and 1250 106.323 - 114.655
Soyabean and sunflower 15, 135 and 1215 98.344 - 106.382

- Sampling method: . Three replicates from low, mid and high dose group, and two replicates from control and vehicle control group samples were processed by transferring 5 grams of test sample in 50 ml of conical flask and 10 ml acetone. and the process was continued by sonicating for 2 minutes. Then the sample were transferred to 15 mL Tarson tube and centrifuged (3000 rpm, at 25°C, 10 min). The supernatant was diluted with acetone, to obtain the final concentration of approximately 0.004 µg/mL of test item and injected to GC-MS/MS.
- Sample storage conditions before analysis: sample was immediately asnalysed soon after sampling
Vehicle:
yes
Remarks:
acetone
Details on preparation and application of test substrate:
The artificial substrate was used as the test medium. Acid washed quartz sand (maximum size of 2 mm in diameter) was used as artificial substrate. Within a given phase of test, all the artificial substrate was from the same source.

Maximum Water Holding Capacity of the Soil
Defined quantity of the acid washed quartz sand was loaded into the Keen’s cup (with filter paper in inner chamber). The Keen’s cup was gradually submerged until the water level is above the top of sand. The Keen’s cup was left in the water for three hours. The sand sample was allowed to drain by placing the Keen’s cup onto a filter paper until water draining on to the filter paper was ceased. The Keen’s cup was then weighed followed by drying to constant mass at 105 ± 5 °C. The maximum water holding capacity (WHCmax) was calculated as 26%
- Controls: Artifical soil
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone
- Concentration of vehicle in test medium (stock solution and final test solution): 10 ml of acetone in 2.3kg of substrate
- Evaporation of vehicle before use: yes
Species:
Allium cepa
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
- Common name: onion
- Plant family: Amaryllidaceae
- Source of seed: Vokkal Seeds Pvt. Ltd
Species:
Zea mays
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
- Common name: corn
- Plant family: Poaceae (Gramineae)
- Source of seed: Vokkal Seeds Pvt. Ltd.
Species:
Helianthus annuus
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
- Common name: Sunflower
- Plant family: Asteraceae (Compositae)
- Source of seed: Vokkal Seeds Pvt. Ltd.
Species:
Brassica oleracea var. capitata
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: cabbage
- Plant family: Brassicaceae (Cruciferae)
- Variety: capitata
- Source of seed: Vokkal Seeds Pvt. Ltd.
Species:
other: Solanum lycopersicon
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: Tomato
- Plant family: Solanaceae
- Source of seed: Vokkal Seeds Pvt. Ltd
Species:
Glycine max (G. soja)
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: soybean
- Plant family: Fabaceae (Leguminosae
- Source of seed: Vokkal Seeds Pvt. Ltd.
Test type:
seedling emergence and seedling growth test
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
21 d
Test temperature:
Onion Tomato: Air temperature range was 19.7 to 22.4 °C
Corn and Cabbage: Air temperature range was 19.6 to 23.0 °C
Soybean and Sunflower: Air temperature range was 19.5 to 23.3 °C
pH:
5.51
Moisture:
26% moisture
Details on test conditions:
TEST SYSTEM
- Testing facility: Laboratory
- Test container (type, material, size):non porous plastic pots, with diameter: 15.5 cm, surface area about: 189 cm2
- Amount of soil: 2kg/pot
- No. of seeds per container:
Plant species Plants per concentration Plants per pot Pots per concentration Planting depth (mm)
Onion 5 20 4 5
Corn 2 20 10 10
Cabbage 3 21 7 5
Tomato 3 21 7 5
Sunflower 3 21 7 10
Soybean 3 21 7 10

- No. of plants (retained after thinning):
- No. of replicates per treatment group: 4 replicates for onion and 10 replicates for corn; 7 replicates for cabbage, tomato, sunflower and soybean
- No. of replicates per control: 4 replicates for onion and 10 replicates for corn; 7 replicates for cabbage, tomato, sunflower and soybean
- No. of replicates per vehicle control: 4 replicates for onion and 10 replicates for corn; 7 replicates for cabbage, tomato, sunflower and soybean
GROWTH CONDITIONS
- Photoperiod: controlled light-dark cycles with 16 hours light and 8 hours dark
- Light source: PAR light (photosynthetically active radiation) wavelength 400 - 700 nm
- Light intensity and quality: between 22600 to 24100 Lux.
- Relative humidity (%): 71 to 89 %.
-Carbondioxide: 340 to 370 ppm
- Watering regime and schedules: Initial top watering was used to stimulate seed germination. After seedling emergence, bottom and top watering (~ 40 to 50 mL/pot sprinkled over artificial substrate and tray) were used. Frequency of watering as well as volume of water used for watering was dependent on artificial substrate moisture content. At any point during watering, water logging situations was avoided


VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3
- Justification for using less concentrations than requested by guideline: test concentrations were used as per test guidelines
- Range finding study
Test concentrations of 10, 100, 500 and 1000 mg/kg dry weight of artificial substrate was tested along with vehicle control during the range finding test.
- Results used to determine the conditions for the definitive study: : growth and development of plant
Nominal and measured concentrations:
Nominal concentrations
Reference substance (positive control):
yes
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
45 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
135 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
405 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Seed survival
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 405 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Seedling Survival
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
45 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
135 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
45 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: shoot weight
Species:
Allium cepa
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
135 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Dry shoot weight
Species:
Zea mays
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
200 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Zea mays
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
500 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Zea mays
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
500 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
Zea mays
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
1 250 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
Zea mays
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
80 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Zea mays
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
200 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Zea mays
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
200 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Dry shoot weight
Species:
Zea mays
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
500 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Dry shoot weight
Species:
Brassica oleracea var. capitata
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
200 ng/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Brassica oleracea var. capitata
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
500 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Brassica oleracea var. capitata
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
1 250 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
Brassica oleracea var. capitata
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 1 250 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
Brassica oleracea var. capitata
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
32 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Brassica oleracea var. capitata
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
80 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Brassica oleracea var. capitata
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
80 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Dry shoot weight
Species:
Brassica oleracea var. capitata
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
200 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Dry shoot weight
Species:
other: Solanum lycopersicon
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
5 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
other: Solanum lycopersicon
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
15 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
other: Solanum lycopersicon
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
405 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
other: Solanum lycopersicon
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
> 405 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
other: Solanum lycopersicon
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
15 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
other: Solanum lycopersicon
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
45 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
other: Solanum lycopersicon
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
15 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: dry shoot weight
Species:
other: Solanum lycopersicon
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
45 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: dry shoot weight
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
135 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
405 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
405 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
1 215 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
15 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
45 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
135 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Dry shoot weight
Species:
Glycine max (G. soja)
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
405 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Dry shoot weight
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
405 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
1 215 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
405 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
1 215 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: seedling survival
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
15 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
45 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
15 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: Dry shoot weight
Species:
Helianthus annuus
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
45 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: dry shoot weight
Details on results:
SEED GERMINATION in control
- Percent seed germination: 100

SEEDLING EMERGENCE
- Percent seedling emergence: 90
- Percent survival: 100
Reported statistics and error estimates:
A dose-response relationship was established in terms of a regression equation by using four parameter Model. Different model was used for estimating ECx (EC10, EC20, EC50, EC90) and its confidence limits, where possible.
The statistical analysis of the shoot height and shoot weight data was evaluated using licensed copies of SYSTAT Statistical package version 12.0. Data was tested for normality (Shapiro-Wilk test) and homogeneity of variance (Levene’s) before performing further analysis. When data was found as normal and homogeneous, ANOVA was performed, when data was found as non-normal or non-homogeneous, data were transformed, and ANOVA was done on transformed data for overall group comparison. Dunnett’s test was performed for pairwise comparison. Comparison of two group’s mean was done using two-sample t-test.
All analyses and comparisons were evaluated at the 5% (p<0.05) level. The unit of expression of endpoints was mg/kg dry weight of artificial substrate.

Range finding test:

Plant species: Onion

Test conc.

(mg/kg)

% of Seedling emergence

% of Seedling survival

% Shoot height reduction

% Dry shoot weight reduction

0 (V)

80.0

100.0

-

-

10

80.0

100.0

2.0

-0.2

100

80.0

100.0

2.0

-0.7

500

50.0

100.0

-45.2

-46.8

1000

30.0

100.0

-50.7

-56.0

Plant species:Corn

0 (V)

75.0

100.0

-

-

10

75.0

100.0

3.3

1.8

100

87.5

100.0

0.8

-1.4

500

62.5

100.0

-53.2

-52.9

1000

50.0

100.0

-78.6

-69.8

Plant species: Cabbage

Test conc.

(mg/kg)

% of Seedling emergence

% of Seedling survival

% Shoot height reduction

% Dry shoot weight reduction

0 (V)

77.8

100.0

-

-

10

77.8

100.0

0.4

1.0

100

100.0

100.0

-0.9

-1.7

500

11.1

100.0

-68.1

-45.4

1000

11.1

100.0

-70.2

-49.0

Plant species:Tomato

0 (V)

100.0

100.0

-

-

10

100.0

100.0

3.9

1.4

100

55.6

100.0

-24.0

-51.6

500

11.1

100.0

-75.1

-79.8

1000

0.0

-

-

-

Plant species: Soybean

0 (V)

100.0

100.0

-

-

10

100.0

100.0

1.3

0.1

100

100.0

100.0

1.5

-2.7

500

55.6

55.6

-75.7

-37.5

1000

22.2

22.2

-79.0

-56.1

Plant species: Sunflower

0 (V)

100.0

100.0

-

-

10

100.0

100.0

1.4

0.3

100

100.0

100.0

1.6

-1.2

500

88.9

88.9

-64.7

-49.1

1000

44.4

44.4

-85.3

-59.2

Definitive test

Plant species: Onion

Test conc.

(mg/kg)

% of Seedling emergence

% of Seedling survival

% Shoot height reduction

% Dry shoot weight reduction

0

90.0

100.0

-

-

0

90.0

100.0

-

-

15

90.0

100.0

0.9

3.1

45

100.0

100.0

-19.4

-20.9

135

80.0

100.0

*-58.7

*-56.7

405

55.0

100.0

*-89.1

*-78.0

1215

0.0

NA

NA

NA

Plant species:Corn

0

90.0

100.0

-

-

0

90.0

100.0

-

-

32

90.0

100.0

0.3

-0.2

80

90.0

100.0

-13.3

-9.2

200

95.0

100.0

*-35.6

-28.8

500

75.0

100.0

*-46.8

*-44.7

1250

35.0

71.4

*-88.0

*-88.2

Plant species: Cabbage

0

90.5

100.0

-

-

0

90.5

100.0

-

-

32

100.0

100.0

-0.1

-0.5

80

95.2

100.0

*-12.7

-16.9

200

90.5

100.0

*-34.2

*-37.8

500

42.9

100.0

*-75.9

*-64.4

1250

19.0

100.0

*-85.9

*-82.3

Plant species:Tomato

0

95.2

100.0

-

-

0

95.2

100.0

-

-

5

95.2

100.0

0.3

-0.2

15

81.0

100.0

-17.3

-13.6

45

66.7

100.0

*-37.8

*-37.5

135

57.1

100.0

*-71.8

*-68.5

405

23.8

100.0

*-86.6

*-81.9

Plant species: Soybean

0

95.2

100.0

-

-

0

95.2

100.0

-

-

15

95.2

100.0

-1.7

0.4

45

100.0

100.0

*-10.8

-6.7

135

95.2

100.0

*-27.1

-24.1

405

90.5

100.0

*-56.8

*-55.4

1215

57.1

83.3

*-94.4

*-88.7

Plant species: Sunflower

Test conc.

(mg/kg)

% of Seedling emergence

% of Seedling survival

% Shoot height reduction

% Dry shoot weight reduction

0

100.0

100.0

-

-

0

95.2

100.0

-

-

15

100.0

100.0

2.9

-1.8

45

95.2

100.0

*-33.4

*-30.0

135

95.2

100.0

*-41.6

*-39.3

405

95.2

100.0

*-70.6

*-67.5

1215

23.8

40.0

*-93.8

*-88.0

Test Item Active Ingredient in Test Medium

Active ingredient

Range of average analyzed content of active ingredient (% of nominal)

Plant species

Test conc.

Day 0

test item

Onion

15, 135 and 1215

81.630 - 93.599

Tomato

5, 45 and 405

92.714 - 96.095

Corn and cabbage

32, 200 and 1250

106.323 - 114.655

Soyabean and sunflower

15, 135 and 1215

98.344 - 106.382

TABLE 1.            Definitive[c1] [r2] Test Conditions - Air Temperature and Relative Humidity

Plant species: Onion and Tomato

Day

Air temperature(°C)

Relative humidity (%)

Minimum

Maximum

Minimum

Maximum

0

19.8

22.0

71

86

1

19.9

22.1

73

88

2

19.8

22.3

72

87

3

19.9

22.4

74

89

4

19.8

22.3

73

87

5

19.7

22.2

72

86

6

19.8

22.1

73

88

7

19.9

22.3

74

87

8

19.8

22.1

73

86

9

19.9

22.2

74

88

10

20.0

22.2

73

87

11

19.9

22.1

74

86

12

20.0

22.1

73

88

13

19.9

22.3

72

87

14

19.8

22.2

71

86

15

19.9

22.1

73

87

16

19.9

22.2

72

89

17

19.8

22.0

73

86

18

19.9

22.1

74

85

19

20.0

22.2

73

84

20

19.9

22.3

74

86

21

20.0

22.2

72

85

Air temperature range was 19.7 to 22.4 °C and relative humidity range was 71 to 89 %.

Plant species: Corn and Cabbage

Day

Air temperature(°C)

Relative humidity (%)

Minimum

Maximum

Minimum

Maximum

0

19.9

23.0

73

84

1

19.8

22.1

76

85

2

19.6

22.9

75

83

3

19.7

22.5

74

81

4

19.6

22.3

76

83

5

19.7

22.2

73

82

6

19.8

22.7

74

83

7

19.6

22.0

75

84

8

19.7

22.3

76

86

9

19.8

22.5

73

89

10

19.6

22.9

75

85

11

19.7

22.2

74

84

12

19.6

22.9

76

81

13

19.8

22.6

73

86

14

19.7

22.6

73

84

15

19.9

22.7

72

83

16

19.6

22.8

75

81

17

19.8

22.9

75

83

18

19.6

22.7

73

81

19

19.8

22.2

75

84

Air temperature range was 19.6 to 23.0 °C and relative humidity range was 72 to 89 %.

Plant species: Soybean and Sunflower

Day

Air temperature(°C)

Relative humidity (%)

Minimum

Maximum

Minimum

Maximum

0

19.9

23.3

76

85

1

19.6

22.3

74

84

2

19.8

22.5

76

85

3

19.7

22.6

75

86

4

19.6

22.4

73

85

5

19.7

22.3

74

84

6

19.8

22.5

73

83

7

19.5

22.3

74

85

8

19.7

22.6

75

86

9

19.6

22.5

76

83

10

19.6

22.6

73

84

11

19.5

22.4

75

86

12

19.7

22.1

76

84

13

19.8

22.3

73

85

14

19.6

22.7

72

84

15

19.5

22.7

75

86

16

19.7

22.7

79

84

17

19.9

22.9

74

85

18

19.7

22.8

72

83

19

19.6

22.3

71

82

Air temperature range was 19.5 to 23.3 °C and relative humidity range was 71 to 86 %.

TABLE 1.            Definitive Test Conditions - Carbon Dioxide and Light Intensity[c1] [r2] 

Plant species: Onion and Tomato

Day

Carbon dioxide in air (ppm)

Light intensity (Lux) range

0

340

22700

23400

22900

23600

1

350

22800

23600

23100

23800

2

350

22600

23500

23200

23600

3

350

23100

23600

22900

23400

4

350

23300

22700

23100

22700

5

350

22900

22800

23200

23100

6

350

22700

23100

22800

23200

7

350

22800

23100

23000

22900

8

350

22900

23000

23100

22800

9

350

22900

22800

23100

23200

10

350

22900

23000

23100

23000

11

350

22800

22900

23000

22900

12

350

22900

23100

23200

23000

13

350

22800

22900

23100

23000

14

350

22900

23000

23100

23000

15

350

22900

22800

23100

23000

16

350

23100

23000

22800

23200

17

350

23000

22900

22800

23100

18

350

22900

22800

23000

23100

19

350

22800

22900

23100

23000

20

350

22900

22900

23200

23100

21

350

22800

22900

23100

23000

Carbon dioxide in air range was 340 to 350 ppm and light intensity range was 22600 to 23800 Lux.

Plant species: Corn and Cabbage

Day

Carbon dioxide in air (ppm)

Light intensity (Lux) range

0

370

23700

23900

23800

24000

1

360

23500

23700

23900

24100

2

350

22900

23100

23700

24000

3

350

22900

23200

23100

23000

4

350

23000

23100

23100

23200

5

350

23100

23000

23100

23300

6

350

23000

23100

23200

22900

7

350

23000

23200

23100

23300

8

350

23400

23200

23100

23300

9

350

23100

23300

23400

23200

10

350

23400

23100

23600

23500

11

360

23100

23000

23100

23300

12

350

23600

23500

23200

23600

13

350

23500

23600

23500

23100

14

350

23100

23000

22900

23000

15

350

23600

23500

23100

23400

16

350

23200

23200

23500

23100

17

350

23200

23100

23300

23400

18

350

24000

23800

23900

23700

19

350

23700

23400

23600

23500

Carbon dioxide in air range was 350 to 370 ppm and light intensity range was 22900 to 24100 Lux.

Plant species: Soybean and Sunflower

Day

Carbon dioxide in air (ppm)

Light intensity (Lux) range

0

340

23100

23400

23200

23700

1

360

23400

22900

23100

23600

2

370

23500

22700

23200

24100

3

350

23200

23100

23300

23100

4

350

23300

23100

23200

23200

5

360

23100

23300

23400

23200

6

350

23500

23300

23700

23400

7

350

23100

23600

23500

23000

8

360

23000

23100

23600

23200

9

350

23400

22900

23000

23200

10

350

23100

23500

23600

23400

11

360

23100

23200

23400

23100

12

350

23200

23600

23400

23200

13

350

23600

23000

23200

23900

14

350

23400

23200

23600

23100

15

350

23700

23500

23600

23300

16

360

23500

23600

23500

23700

17

350

23300

23200

23000

23400

18

350

23400

23200

22900

23100

19

350

23500

23100

23300

23200

Carbon dioxide in air range was 340 to 370 ppm and light intensity range was 22700 to 24100 Lux.

TABLE 1.            Onion DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

8-Aug

9-Aug

10-Aug

11-Aug

12-Aug

13-Aug

14-Aug

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

21-Aug

28-Aug

21-Aug

28-Aug

CR1

4

90.0

4

18

100.0

0

0

0

0

0

1

4

4

4

N(4), 0%(4)

N(4), 0%(4)

CR2

5

5

0

0

0

0

0

2

5

5

5

N(5), 0%(5)

N(5), 0%(5)

CR3

5

5

0

0

0

0

0

1

5

5

5

N(5), 0%(5)

N(5), 0%(5)

CR4

4

4

0

0

0

0

0

1

4

4

4

N(4), 0%(4)

N(4), 0%(4)

VR1

5

90.0

5

18

100.0

0

0

0

0

0

1

5

5

5

N(5), 0%(5)

N(5), 0%(5)

VR2

5

5

0

0

0

0

0

1

5

5

5

N(5), 0%(5)

N(5), 0%(5)

VR3

4

4

0

0

0

0

0

0

4

4

4

N(4), 0%(4)

N(4), 0%(4)

VR4

4

4

0

0

0

0

0

0

4

4

4

N(4), 0%(4)

N(4), 0%(4)

T1R1

5

90.0

5

18

100.0

0

0

0

0

0

0

5

5

5

N(5), 0%(5)

N(5), 0%(5)

T1R2

5

5

0

0

0

0

0

1

5

5

5

N(5), 0%(5)

N(5), 0%(5)

T1R3

4

4

0

0

0

0

0

1

4

4

4

N(4), 0%(4)

N(4), 0%(4)

T1R4[YB3] 

4

4

0

0

0

0

0

1

4

4

4

N(4), 0%(4)

N(4), 0%(4)

T2R1

5

100.0

5

20

100.0

0

0

0

0

0

0

5

5

5

S(5), 40%(5)

S(5), 40%(5)

T2R2

5

5

0

0

0

0

0

0

5

5

5

N(5), 0%(5)

N(5), 0%(5)

T2R3

5

5

0

0

0

0

0

0

5

5

5

S(5), 25%(5)

S(5), 25%(5)

T2R4

5

5

0

0

0

0

0

1

5

5

5

N(5), 0%(5)

N(5), 0%(5)

T3R1

3

80.0

3

16

100.0

0

0

0

0

0

0

3

3

3

S(3), 50%(3)

S(3), 50%(3)

T3R2

5

5

0

0

0

0

0

0

5

5

5

S(5), 50%(5)

S(5), 50%(5)

T3R3

4

4

0

0

0

0

0

1

4

4

4

S(4), 60%(3), 90%(1)

S(4), 60%(3), 90%(1)

T3R4

4

4

0

0

0

0

0

0

4

4

4

S(4), 70%(4)

S(4), 70%(4)

T4R1

4

55.0

4

11

100.0

0

0

0

0

0

0

4

4

4

S(4), 90%(4)

S(4), 90%(4)

T4R2

3

3

0

0

0

0

0

0

3

3

3

S(3), 90%(3)

S(3), 90%(3)

T4R3

2

2

0

0

0

0

0

0

2

2

2

S(2), 90%(2)

S(2), 90%(2)

T4R4

2

2

0

0

0

0

0

0

2

2

2

S(2), 90%(2)

S(2), 90%(2)

T5R1

0

0.0

-

-

-

0

0

0

0

0

0

0

0

0

-

-

T5R2

0

-

0

0

0

0

0

0

0

0

0

-

-

T5R3

0

-

0

0

0

0

0

0

0

0

0

-

-

T5R4

0

-

0

0

0

0

0

0

0

0

0

-

-

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.

Onion DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Dry shoot weight (g) on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Group dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

3

4

5

Average

28-Aug

29-Aug

CR1

8.5

11.0

9.7

9.8

-

9.8

0.0251

25.1

-

0.1164

0.0065

-

CR2

8.8

11.2

9.6

10.4

9.6

9.9

0.0318

31.8

CR3

9.3

10.6

11.2

8.6

11.3

10.2

0.0332

33.2

CR4

9.8

9.6

8.8

11.4

-

9.9

0.0263

26.3

VR1

10.3

9.8

9.5

8.6

9.6

9.6

0.0312

31.2

-

0.1176

0.0065

-

VR2

10.6

10.3

11.3

9.3

10.7

10.4

0.0329

32.9

VR3

9.9

11.0

10.4

10.8

-

10.5

0.0274

27.4

VR4

8.8

10.1

8.7

10.3

-

9.5

0.0261

26.1

T1R1

10.4

9.6

10.1

10.3

10.6

10.2

0.0327

32.7

0.9[YB3] 

0.1212

0.0067

3.1

T1R2

10.7

11.0

8.6

11.3

11.2

10.6

0.0338

33.8

T1R3

9.6

9.5

9.8

10.4

-

9.8

0.0276

27.6

T1R4

10.2

10.4

8.9

9.6

-

9.8

0.0271

27.1

T2R1

5.8

5.5

6.0

5.9

5.4

5.7

0.0183

18.3

-19.4

0.1034

0.0052

-20.9

T2R2

10.3

11.0

9.8

8.4

9.2

9.7

0.0314

31.4

T2R3

7.2

7.3

7.7

7.1

6.8

7.2

0.0238

23.8

T2R4

11.0

9.1

8.6

10.2

9.0

9.6

0.0299

29.9

T3R1

5.5

4.8

5.0

-

-

5.1

0.0096

9.6

-58.7*

0.0453

0.0028

-56.7*

T3R2

4.6

4.9

5.1

5.0

5.4

5.0

0.0164

16.4

T3R3

1.5

4.0

4.3

3.8

-

3.4

0.0104

10.4

T3R4

3.0

2.8

3.3

3.0

-

3.0

0.0089

8.9

T4R1

1.2

0.9

1.1

1.4

-

1.2

0.0064

6.4

-89.1*

0.0158

0.0014

-78.0*

T4R2

1.0

1.2

0.8

-

-

1.0

0.0043

4.3

T4R3

1.4

0.9

-

-

-

1.2

0.0029

2.9

T4R4

0.8

1.3

-

-

-

1.1

0.0022

2.2

T5R1

-

-

-

-

-

-

-

-

-

-

-

-

T5R2

-

-

-

-

-

-

-

-

T5R3

-

-

-

-

-

-

-

-

T5R4

-

-

-

-

-

-

-

-

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1.            Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

 Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

Seedling emergence

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

18-Sep

19-Sep

20-Sep

21-Sep

22-Sep

29-Sep

06-Oct

29-Sep

06-Oct

CR1

2

90.0

2

18

100.0

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR2

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR3

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR4

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR5

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR6

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR7

1

1

0

0

0

0

1

1

1

N(1), 0%(1)

N(1), 0%(1)

CR8

1

1

0

0

0

0

1

1

1

N(1), 0%(1)

N(1), 0%(1)

CR9

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR10

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR1

1

90.0

1

18

100.0

0

0

0

0

1

1

1

N(1), 0%(1)

N(1), 0%(1)

VR2

1

1

0

0

0

0

1

1

1

N(1), 0%(1)

N(1), 0%(1)

VR3

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR4

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR5

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR6

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR7

2

2

0

0

0

1

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR8

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR9

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR10

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal.

Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Shoot weight on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

Average

06-Oct

07-Oct

CR1

15.9

16.4

16.2

0.7811

781.1

-

6.6223

0.3679

-

CR2

15.0

15.4

15.2

0.7323

732.3

CR3

16.6

15.2

15.9

0.7531

753.1

CR4

14.6

14.4

14.5

0.7118

711.8

CR5

13.9

15.7

14.8

0.7362

736.2

CR6

13.0

12.9

13.0

0.7034

703.4

CR7

16.1

-

16.1

0.3711

371.1

CR8

15.2

-

15.2

0.3562

356.2

CR9

14.3

14.2

14.3

0.7339

733.9

CR10

13.8

16.1

15.0

0.7432

743.2

VR1

14.6

-

14.6

0.3666

366.6

-

6.6615

0.3701

-

VR2

15.9

-

15.9

0.3775

377.5

VR3

14.3

13.9

14.1

0.7372

737.2

VR4

15.9

15.4

15.7

0.7443

744.3

VR5

16.0

15.2

15.6

0.7556

755.6

VR6

14.8

14.6

14.7

0.7118

711.8

VR7

15.2

14.6

14.9

0.7231

723.1

VR8

13.7

14.8

14.3

0.7311

731.1

VR9

16.2

15.3

15.8

0.7465

746.5

VR10

14.5

15.1

14.8

0.7678

767.8

Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

 Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

18-Sep

19-Sep

20-Sep

21-Sep

22-Sep

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

29-Sep

06-Oct

29-Sep

06-Oct

T1R1

1

90.0

1

18

100.0

0

0

0

0

1

1

1

N(1), 0%(1)

N(1), 0%(1)

T1R2

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R3

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R4

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R5

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R6

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R7

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R8

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R9

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R10

1

1

0

0

0

0

1

1

1

N(1), 0%(1)

N(1), 0%(1)

T2R1

2

90.0

2

18

100.0

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T2R2

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T2R3

1

1

0

0

0

0

1

1

1

N(1), 0%(1)

N(1), 0%(1)

T2R4

2

2

0

0

0

0

2

2

2

S(2), 20%(2)

S(2), 20%(2)

T2R5

2

2

0

0

0

0

2

2

2

S(2), 20%(2)

S(2), 20%(2)

T2R6

1

1

0

0

0

0

1

1

1

S(1), 30%(1)

S(1), 30%(1)

T2R7

2

2

0

0

0

0

2

2

2

S(2), 60%(1), 30%(1)

S(2), 60%(1), 30%(1)

T2R8

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T2R9

2

2

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T2R10

2

2

0

0

0

0

2

2

2

N(1), S(1), 0%(1), 20%(1)

N(1), S(1), 0%(1), 20%(1)

T3R1

2

95.0

2

19

100.0

0

0

0

0

1

2

2

S(2), 20%(1), 50%(1)

S(2), 20%(1), 50%(1)

T3R2

2

2

0

0

0

0

2

2

2

S(2), 20%(1), 30%(1)

S(2), 20%(1), 30%(1)

T3R3

2

2

0

0

0

0

0

2

2

S(2), 30%(2)

S(2), 30%(2)

T3R4

2

2

0

0

0

0

0

2

2

S(2), 10%(1), 40%(1)

S(2), 10%(1), 40%(1)

T3R5

2

2

0

0

0

0

1

2

2

S(2), 20%(1), 50%(1)

S(2), 20%(1), 50%(1)

T3R6

2

2

0

0

0

0

1

2

2

S(2), 30%(1), 60%(1)

S(2), 30%(1), 60%(1)

T3R7

2

2

0

0

0

0

1

2

2

S(2), 30%(1), 50%(1)

S(2), 30%(1), 50%(1)

T3R8

2

2

0

0

0

0

0

2

2

S(2), 20%(1), 40%(1)

S(2), 20%(1), 40%(1)

T3R9

1

1

0

0

0

0

0

1

1

S(1), 60%(1)

S(1), 60%(1)

T3R10

2

2

0

0

0

0

0

2

2

S(2), 30%(1), 40%(1)

S(2), 30%(1), 40%(1)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.

contd. Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Shoot weight on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

Average

06-Oct

07-Oct

T1R1

13.9

-

13.9

0.3608

360.8

0.3[YB3] 

6.6507

0.3695

-0.2

T1R2

14.2

14.8

14.5

0.7426

742.6

T1R3

15.3

13.7

14.5

0.7228

722.8

T1R4

16.0

13.9

15.0

0.7263

726.3

T1R5

15.8

14.8

15.3

0.7334

733.4

T1R6

14.7

15.5

15.1

0.7372

737.2

T1R7

15.3

15.8

15.6

0.7469

746.9

T1R8

16.2

15.3

15.8

0.7581

758.1

T1R9

15.3

14.9

15.1

0.7456

745.6

T1R10

16.0

-

16.0

0.3770

377.0

T2R1

13.2

14.0

13.6

0.6989

698.9

-13.3

6.0488

0.3360

-9.2

T2R2

15.2

14.5

14.9

0.7315

731.5

T2R3

15.0

-

15.0

0.3531

353.1

T2R4

11.6

12.2

11.9

0.6583

658.3

T2R5

10.7

12.0

11.4

0.6376

637.6

T2R6

11.0

-

11.0

0.2894

289.4

T2R7

11.1

6.4

8.8

0.4565

456.5

T2R8

16.2

16.4

16.3

0.7956

795.6

T2R9

14.5

15.4

15.0

0.7544

754.4

T2R10

13.9

11.1

12.5

0.6735

673.5

T3R1

12.2

7.3

9.8

0.5321

532.1

-35.6*

5.0094

0.2637

-28.8

T3R2

11.9

11.2

11.6

0.6401

640.1

T3R3

11.0

11.4

11.2

0.6119

611.9

T3R4

9.6

12.4

11.0

0.5993

599.3

T3R5

12.0

7.3

9.7

0.5282

528.2

T3R6

10.6

5.0

7.8

0.3892

389.2

T3R7

11.1

8.0

9.6

0.5126

512.6

T3R8

11.4

10.0

10.7

0.5578

557.8

T3R9

5.5

-

5.5

0.1165

116.5

T3R10

9.6

10.6

10.1

0.5217

521.7

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

contd. Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

 Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

18-Sep

19-Sep

20-Sep

21-Sep

22-Sep

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

29-Sep

06-Oct

29-Sep

06-Oct

T4R1

2

75.0

2

15

100.0

0

0

0

1

1

2

2

S(2), 40%(1), 60%(1)

S(2), 40%(1), 60%(1)

T4R2

1

1

0

0

0

0

0

1

1

S(1), 80%(1)

S(1), 80%(1)

T4R3

1

1

0

0

0

0

0

1

1

S(1), 80%(1)

S(1), 80%(1)

T4R4

1

1

0

0

0

0

1

1

1

S(1), 40%(1)

S(1), 40%(1)

T4R5

2

2

0

0

0

0

2

2

2

S(2), 40%(1), 60%(1)

S(2), 40%(1), 60%(1)

T4R6

1

1

0

0

0

0

0

1

1

S(1), 40%(1)

S(1), 40%(1)

T4R7

2

2

0

0

0

0

1

2

2

S(2), 50%(2)

S(2), 50%(2)

T4R8

2

2

0

0

0

0

2

2

2

S(2), 40%(2)

S(2), 40%(2)

T4R9

2

2

0

0

0

0

1

2

2

S(2), 10%(1), 80%(1)

S(2), 10%(1), 80%(1)

T4R10

1

1

0

0

0

0

1

1

1

S(1), 40%(1)

S(1), 40%(1)

T5R1

2

35.0

0

5

71.4

0

0

0

0

0

2

2

S(2), 80%(2)

D(2), 100%(2)

T5R2

2

2

0

0

0

0

0

2

2

S(2), 90%(1), 80%(1)

S(2), 90%(1), 80%(1)

T5R3

1

1

0

0

0

0

0

1

1

S(1), 90%(1)

S(1), 90%(1)

T5R4

-

-

0

0

0

-

-

-

-

-

-

T5R5

-

-

0

0

0

-

-

-

-

-

-

T5R6

1

1

0

0

0

0

0

1

1

S(1), 80%(1)

S(1), 80%(1)

T5R7

1

1

0

0

0

0

0

1

1

S(1), 95%(1)

S(1), 95%(1)

T5R8

-

-

0

0

0

-

-

-

-

-

-

T5R9

-

-

0

0

0

-

-

-

-

-

-

T5R10

-

-

0

0

0

-

-

-

-

-

-

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth; D: Dead.

Corn DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Shoot weight on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

Average

06-Oct

07-Oct

T4R1

10.0

7.0

8.5

0.4217

421.7

-46.8*

3.0692

0.2046

-44.7*

T4R2

2.0

-

2.0

0.0641

64.1

T4R3

2.4

-

2.4

0.0863

86.3

T4R4

11

-

11.0

0.2453

245.3

T4R5

7.0

11.0

9.0

0.4701

470.1

T4R6

9.9

-

9.9

0.2193

219.3

T4R7

8.3

8.5

8.4

0.3995

399.5

T4R8

11.0

9.4

10.2

0.4867

486.7

T4R9

13.0

2.0

7.5

0.3981

398.1

T4R10

11.0

-

11.0

0.2781

278.1

T5R1

-

-

-

-

-

-88.0*

0.2178

0.0436

-88.2*

T5R2

1.5

2.3

1.9

0.0932

93.2

T5R3

1.7

-

1.7

0.0381

38.1

T5R4

-

-

-

-

-

T5R5

-

-

-

-

-

T5R6

2.8

-

2.8

0.0632

63.2

T5R7

0.8

-

0.8

0.0233

23.3

T5R8

-

-

-

-

-

T5R9

-

-

-

-

-

T5R10

-

-

-

-

-

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1.            Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

 Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

18-Sep

19-Sep

20-Sep

21-Sep

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

28-Sep

05-Oct

28-Sep

05-Oct

CR1

2

90.5

2

19

100.0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR2

3

3

0

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

CR3

3

3

0

0

0

2

3

3

N(3), 0%(3)

N(3), 0%(3)

CR4

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR5

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR6

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR7

2

2

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR1

1

90.5

1

19

100.0

0

0

0

1

1

1

N(1), 0%(1)

N(1), 0%(1)

VR2

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR3

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR4

3

3

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR5

3

3

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR6

3

3

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR7

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R1

3

100.0

3

21

100.0

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R2

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R3

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R4

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R5

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R6

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R7

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T2R1

3

95.2

3

20

100.0

0

0

0

1

3

3

S(3), 20%(3)

S(3), 20%(3)

T2R2

3

3

0

0

0

2

3

3

S(3), 30%(3)

S(3), 30%(3)

T2R3

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T2R4

2

2

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T2R5

3

3

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T2R6

3

3

0

0

0

3

3

3

S(3), 10%(3)

S(3), 10%(3)

T2R7

3

3

0

0

0

3

3

3

S(3), 10%(3)

S(3), 10%(3)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.

contd. Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Dry shoot weight (g) on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

3

Average

05-Oct

06-Oct

CR1

4.2

4.0

-

4.1

0.0888

88.8

-

0.8300

0.0437

-

CR2

4.3

4.7

4.2

4.4

0.1304

130.4

CR3

4.2

4.1

4.4

4.2

0.1222

122.2

CR4

4.1

4.2

4.6

4.3

0.1342

134.2

CR5

4.2

4.4

3.9

4.2

0.1265

126.5

CR6

3.8

4.9

4.5

4.4

0.1378

137.8

CR7

4.0

3.9

-

4.0

0.0901

90.1

VR1

4.7

-

-

4.7

0.0467

46.7

-

0.8363

0.0440

-

VR2

3.8

4.1

4.4

4.1

0.1236

123.6

VR3

4.2

3.9

4.8

4.3

0.1379

137.9

VR4

3.9

3.7

4.5

4.0

0.1194

119.4

VR5

4.8

4.8

4.9

4.8

0.1462

146.2

VR6

4.7

4.9

4.2

4.6

0.1335

133.5

VR7

4.2

4.0

3.9

4.0

0.1290

129.0

T1R1

3.8

4.8

4.3

4.3

0.1316

131.6

-0.1

0.9199

0.0438

-0.5

T1R2

4.2

4.6

3.9

4.2

0.1329

132.9

T1R3

4.4

3.9

4.8

4.4

0.1388

138.8

T1R4

4.7

4.0

4.9

4.5

0.1372

137.2

T1R5

3.9

4.7

3.8

4.1

0.1194

119.4

T1R6

4.2

4.6

4.9

4.6

0.1364

136.4

T1R7

4.2

4.9

4.2

4.4

0.1236

123.6

T2R1

3.0

3.8

3.9

3.6

0.1023

102.3

-12.7*

0.7317

0.0366

-16.9

T2R2

3.4

3.5

2.9

3.3

0.0973

97.3

T2R3

4.1

3.9

4.3

4.1

0.1227

122.7

T2R4

4.0

4.0

-

4.0

0.0822

82.2

T2R5

3.8

4.4

4.1

4.1

0.1193

119.3

T2R6

3.8

3.9

4.0

3.9

0.0983

98.3

T2R7

3.9

3.6

3.8

3.8

0.1096

109.6

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

. Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

 Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

18-Sep

19-Sep

20-Sep

21-Sep

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

28-Sep

05-Oct

28-Sep

05-Oct

T3R1

3

90.5

3

19

100.0

0

0

0

2

3

3

S(3), 40%(3)

S(3), 40%(3)

T3R2

2

2

0

0

0

2

2

2

S(2), 40%(2)

S(2), 40%(2)

T3R3

2

2

0

0

0

2

2

2

S(2), 50%(2)

S(2), 50%(2)

T3R4

3

3

0

0

0

3

3

3

S(3), 20%(2), 50%(1)

S(3), 20%(2), 50%(1)

T3R5

3

3

0

0

0

2

3

3

S(3), 30%(3)

S(3), 30%(3)

T3R6

3

3

0

0

0

3

3

3

S(3), 30%(2), 60%(1)

S(3), 30%(2), 60%(1)

T3R7

3

3

0

0

0

3

3

3

S(3), 30%(3)

S(3), 30%(3)

T4R1

0

42.9

0

9

100.0

0

0

0

0

0

0

-

-

T4R2

0

0

0

0

0

0

0

0

-

-

T4R3

3

3

0

0

0

2

3

3

S(3), 70%(3)

S(3), 70%(3)

T4R4

3

3

0

0

0

0

3

3

S(3), 90%(3)

S(3), 90%(3)

T4R5

3

3

0

0

0

1

3

3

S(3), 80%(3)

S(3), 80%(3)

T4R6

0

0

0

0

0

0

0

0

-

-

T4R7

0

0

0

0

0

0

0

0

-

-

T5R1

0

19.0

0

4

100.0

0

0

0

0

0

0

-

-

T5R2

2

2

0

0

0

0

2

2

S(2), 95%(2)

S(2), 95%(2)

T5R3

0

0

0

0

0

0

0

0

-

-

T5R4

0

0

0

0

0

0

0

0

-

-

T5R5

1

1

0

0

0

0

1

1

S(1), 90%(1)

S(1), 90%(1)

T5R6

0

0

0

0

0

0

0

0

-

-

T5R7

1

1

0

0

0

0

1

1

S(1), 90%(1)

S(1), 90%(1)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth.

Cabbage DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Dry shoot weight (g) on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

3

Average

05-Oct

06-Oct

T3R1

2.9

2.6

3.3

2.9

0.0822

82.2

-34.2*

0.5203

0.0274

-37.8*

T3R2

2.9

2.6

-

2.8

0.0514

51.4

T3R3

2.9

2.2

-

2.6

0.0496

49.6

T3R4

3.3

3.4

2.6

3.1

0.0864

86.4

T3R5

2.9

3.0

3.2

3.0

0.0884

88.4

T3R6

3.1

2.9

2.2

2.7

0.0744

74.4

T3R7

3.3

3.0

2.8

3.0

0.0879

87.9

T4R1

-

-

-

-

-

-

-75.9*

0.1410

0.0157

-64.4*

T4R2

-

-

-

-

-

-

T4R3

1.6

1.8

1.3

1.6

0.0686

68.6

T4R4

0.5

0.4

0.6

0.5

0.0251

25.1

T4R5

0.9

1.1

1.3

1.1

0.0473

47.3

T4R6

-

-

-

-

-

-

T4R7

-

-

-

-

-

-

T5R1

-

-

-

-

-

-

-85.9*

0.0312

0.0078

-82.3*

T5R2

0.4

0.7

-

0.6

0.0163

16.3

T5R3

-

-

-

-

-

-

T5R4

-

-

-

-

-

-

T5R5

0.8

-

-

0.8

0.0088

8.8

T5R6

-

-

-

-

-

-

T5R7

0.5

-

-

0.5

0.0061

6.1

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1.            Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

 Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

8-Aug

9-Aug

10-Aug

11-Aug

12-Aug

13-Aug

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

20-Aug

27-Aug

20-Aug

27-Aug

CR1

3

95.2

3

20

100.0

0

0

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR2

3

3

0

0

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR3

3

3

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR4

3

3

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR5

2

2

0

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR6

3

3

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR7

3

3

0

0

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR1

3

95.2

3

20

100.0

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR2

2

2

0

0

0

0

1

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR3

3

3

0

0

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR4

3

3

0

0

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR5

3

3

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR6

3

3

0

0

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR7

3

3

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R1

3

95.2

3

20

100.0

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R2

3

3

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R3

2

2

0

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R4

3

3

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R5

3

3

0

0

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R6

3

3

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R7

3

3

0

0

0

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T2R1

3

81.0

3

17

100.0

0

0

0

0

0

3

3

3

S(3), 10%(2), 30%(1)

S(3), 10%(2), 30%(1)

T2R2

3

3

0

0

0

0

1

3

3

3

N(2), 0%(2), S(1), 25%(1)

N(2), 0%(2), S(1), 25%(1)

T2R3

3

3

0

0

0

0

1

3

3

3

N(3), 0%(3)

N(3), 0%(3)

T2R4

2

2

0

0

0

0

1

2

2

2

N(1), 0%(1), S(1), 35%(1)

N(1), 0%(1), S(1), 35%(1)

T2R5

2

2

0

0

0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

T2R6

2

2

0

0

0

0

1

2

2

2

N(1), 0%(1), S(1), 45%(1)

N(1), 0%(1), S(1), 45%(1)

T2R7

2

2

0

0

0

0

1

2

2

2

S(2), 30%(2)

S(2), 30%(2)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; N: Normal, S:Stunted growth.

contd. Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Shoot weight on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

3

Average

27-Aug

28-Aug

CR1

11.0

10.5

10.0

10.5

0.2212

221.2

-

1.4785

0.0739

-

CR2

10.0

10.5

10.0

10.2

0.2311

231.1

CR3

10.5

8.0

9.5

9.3

0.2202

220.2

CR4

9.5

11.0

10.5

10.3

0.2116

211.6

CR5

9.0

8.2

-

8.6

0.1347

134.7

CR6

10.5

9.5

11.0

10.3

0.2289

228.9

CR7

10.5

11.0

12.5

11.3

0.2308

230.8

VR1

10.2

9.7

8.9

9.6

0.2191

219.1

-

1.4834

0.0742

-

VR2

9.9

10.7

-

10.3

0.1404

140.4

VR3

11.0

9.9

9.6

10.2

0.2316

231.6

VR4

9.9

9.6

10.6

10.0

0.2129

212.9

VR5

10.4

10.6

9.4

10.1

0.2197

219.7

VR6

10.9

9.7

10.7

10.4

0.2306

230.6

VR7

9.8

10.3

9.8

10.0

0.2291

229.1

T1R1

10.3

9.9

10.2

10.1

0.2214

221.4

0.3[YB3] 

1.4809

0.0740

-0.2

T1R2

10.1

10.2

9.5

9.9

0.2146

214.6

T1R3

10.6

10.1

-

10.4

0.1469

146.9

T1R4

9.9

11.0

10.3

10.4

0.2389

238.9

T1R5

8.9

10.6

10.5

10.0

0.2169

216.9

T1R6

10.8

9.7

9.9

10.1

0.2214

221.4

T1R7

10.2

9.2

10.4

9.9

0.2208

220.8

T2R1

8.1

8.0

6.8

7.6

0.1823

182.3

-17.3

1.0889

0.0641

-13.6

T2R2

9.2

9.4

7.1

8.6

0.2006

200.6

T2R3

9.5

9.8

10.1

9.8

0.2246

224.6

T2R4

9.2

6.6

-

7.9

0.1179

117.9

T2R5

9.7

9.3

-

9.5

0.1486

148.6

T2R6

9.8

6.3

-

8.1

0.1144

114.4

T2R7

7.1

6.8

-

7.0

0.1005

100.5

contd. Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

 Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

8-Aug

9-Aug

10-Aug

11-Aug

12-Aug

13-Aug

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

20-Aug

27-Aug

20-Aug

27-Aug

T3R1

3

66.7

3

14

100.0

0

0

0

0

0

0

3

3

S(3), 60%(3)

S(3), 60%(3)

T3R2

2

2

0

0

0

0

0

0

2

2

S(2), 50%(2)

S(2), 50%(2)

T3R3

1

1

0

0

0

0

0

0

1

1

S(1), 55%(1)

S(1), 55%(1)

T3R4

1

1

0

0

0

0

0

1

1

1

S(1), 50%(1)

S(1), 50%(1)

T3R5

3

3

0

0

0

0

0

1

3

3

S(3), 60%(3)

S(3), 60%(3)

T3R6

2

2

0

0

0

0

0

0

2

2

S(2), 50%(2)

S(2), 50%(2)

T3R7

2

2

0

0

0

0

0

0

2

2

S(2), 60%(2)

S(2), 60%(2)

T4R1

3

57.1

3

12

100.0

0

0

0

0

0

1

3

3

S(3), 80%(3)

S(3), 80%(3)

T4R2

2

2

0

0

0

0

0

0

0

2

-

S(2), 80%(2)

T4R3

1

1

0

0

0

0

0

0

1

1

S(1), 70%(1)

S(1), 70%(1)

T4R4

0

0

0

0

0

0

0

0

0

0

-

-

T4R5

2

2

0

0

0

0

0

0

1

2

S(1), 65%(1)

S(2), 65%(1), 70%(1)

T4R6

3

3

0

0

0

0

0

0

2

3

S(2), 65%(2)

S(3), 65%(2), 75%(1)

T4R7

1

1

0

0

0

0

0

0

1

1

S(1), 50%(1)

S(1), 50%(1)

T5R1

0

23.8

0

5

100.0

0

0

0

0

0

0

0

0

-

-

T5R2

0

0

0

0

0

0

0

0

0

0

-

-

T5R3

1

1

0

0

0

0

0

0

1

1

S(1), 85%(1)

S(1), 85%(1)

T5R4

1

1

0

0

0

0

0

0

0

1

-

S(1), 90%(1)

T5R5

1

1

0

0

0

0

0

0

0

1

-

S(1), 90%(1)

T5R6

0

0

0

0

0

0

0

0

0

0

-

-

T5R7

2

2

0

0

0

0

0

0

0

2

-

S(2), 85%(2)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth.

Tomato DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Shoot weight on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

3

Average

27-Aug

28-Aug

T3R1

5.8

5.6

4.3

5.2

0.1146

114.6

-37.8*

0.6490

0.0464

-37.5*

T3R2

6.6

7.1

-

6.9

0.1006

100.6

T3R3

6.2

-

-

6.2

0.0454

45.4

T3R4

6.8

-

-

6.8

0.0576

57.6

T3R5

5.9

6.3

6.7

6.3

0.1396

139.6

T3R6

7.6

6.6

-

7.1

0.1067

106.7

T3R7

6.0

4.9

-

5.5

0.0845

84.5

T4R1

2.0

2.1

2.2

2.1

0.0661

66.1

-71.8*

0.2802

0.0234

-68.5*

T4R2

1.8

1.9

-

1.9

0.0243

24.3

T4R3

2.8

-

-

2.8

0.0189

18.9

T4R4

-

-

-

-

-

-

T4R5

3.3

2.8

-

3.1

0.0501

50.1

T4R6

3.2

3.4

2.3

3.0

0.0897

89.7

T4R7

4.3

-

-

4.3

0.0311

31.1

T5R1

-

-

-

-

-

-

-86.6*

0.0673

0.0135

-81.9*

T5R2

-

-

-

-

-

-

T5R3

1.6

-

-

1.6

0.0150

15.0

T5R4

1.3

-

-

1.3

0.0131

13.1

T5R5

1.1

-

-

1.1

0.0110

11.0

T5R6

-

-

-

-

-

-

T5R7

1.5

1.3

-

1.4

0.0282

28.2

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1.            Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

18-Sep

19-Sep

20-Sep

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

27-Sep

04-Oct

27-Sep

04-Oct

CR1

2

95.2

2

20

100.0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

CR2

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR3

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR4

3

3

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

CR5

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR6

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR7

3

3

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

VR1

2

95.2

2

20

100.0

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR2

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR3

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR4

3

3

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

VR5

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR6

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR7

3

3

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R1

3

95.2

3

20

100.0

0

0

0

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R2

3

3

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R3

2

2

0

0

1

2

2

N(2), 0%(2)

N(2), 0%(2)

T1R4

3

3

0

0

2

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R5

3

3

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R6

3

3

0

0

2

3

3

N(3), 0%(3)

N(3), 0%(3)

T1R7

3

3

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

T2R1

3

100.0

3

21

100.0

0

0

2

3

3

S(3), 10%(3)

S(3), 10%(3)

T2R2

3

3

0

0

0

3

3

S(3), 10%(3)

S(3), 10%(3)

T2R3

3

3

0

0

1

3

3

S(3), 10%(3)

S(3), 10%(3)

T2R4

3

3

0

0

0

3

3

S(3), 15%(3)

S(3), 15%(3)

T2R5

3

3

0

0

0

3

3

S(3), 10%(3)

S(3), 10%(3)

T2R6

3

3

0

0

1

3

3

S(3), 10%(3)

S(3), 10%(3)

T2R7

3

3

0

0

0

3

3

S(3), 10%(3)

S(3), 10%(3)

. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Shoot weight on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

3

Average

04-Oct

05-Oct

CR1

30.5

30.7

-

30.6

0.4672

467.2

-

4.6632

0.2332

-

CR2

27.1

30.1

31.9

29.7

0.7096

709.6

CR3

30.5

29.2

28.1

29.3

0.6998

699.8

CR4

30.6

27.8

32.5

30.3

0.7089

708.9

CR5

29.8

29.4

30.6

29.9

0.7008

700.8

CR6

31.4

30.0

28.6

30.0

0.7000

700.0

CR7

27.9

30.3

28.6

28.9

0.6769

676.9

VR1

30.5

30.7

-

30.6

0.4673

467.3

-

4.6945

0.2347

-

VR2

27.1

30.1

31.9

29.7

0.7096

709.6

VR3

30.5

29.2

28.1

29.3

0.6999

699.9

VR4

30.6

27.8

32.5

30.3

0.7089

708.9

VR5

29.8

29.4

30.6

29.9

0.7008

700.8

VR6

31.5

30.0

28.6

30.0

0.7210

721.0

VR7

27.9

30.3

28.6

28.9

0.6870

687.0

T1R1

30.1

29.7

28.1

29.3

0.7104

710.4

-1.7

4.7124

0.2356

0.4[YB3] 

T1R2

28.7

30.8

30.4

30.0

0.7223

722.3

T1R3

30.1

28.9

-

29.5

0.4776

477.6

T1R4

30.2

28.8

30.1

29.7

0.7021

702.1

T1R5

28.9

29.0

28.6

28.8

0.7016

701.6

T1R6

28.6

30.2

27.9

28.9

0.6913

691.3

T1R7

28.9

29.1

29.0

29.0

0.7071

707.1

T2R1

25.9

26.1

26.4

26.1

0.6400

640.0

-10.8*

4.5977

0.2189

-6.7

T2R2

25.8

25.3

25.9

25.7

0.6220

622.0

T2R3

26.2

27.3

26.9

26.8

0.6757

675.7

T2R4

25.1

25.2

25.9

25.4

0.6367

636.7

T2R5

26.1

28.6

28.3

27.7

0.6806

680.6

T2R6

27.3

28.2

25.6

27.0

0.6646

664.6

T2R7

28.3

26.8

27.6

27.6

0.6781

678.1

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

contd. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

18-Sep

19-Sep

20-Sep

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

27-Sep

04-Oct

27-Sep

04-Oct

T3R1

3

95.2

3

20

100.0

0

0

1

3

3

S(3), 30%(3)

S(3), 30%(3)

T3R2

3

3

0

0

2

3

3

S(3), 25%(3)

S(3), 25%(3)

T3R3

3

3

0

0

2

3

3

S(3), 25%(3)

S(3), 25%(3)

T3R4

3

3

0

0

2

3

3

S(3), 30%(3)

S(3), 30%(3)

T3R5

3

3

0

0

0

3

3

S(3), 25%(3)

S(3), 25%(3)

T3R6

2

2

0

0

2

2

2

S(2), 25%(2)

S(2), 25%(2)

T3R7

3

3

0

0

0

3

3

S(3), 25%(3)

S(3), 25%(3)

T4R1

3

90.5

3

19

100.0

0

0

0

3

3

S(3), 60%(3)

S(3), 60%(3)

T4R2

2

2

0

0

0

2

2

S(2), 60%(2)

S(2), 60%(2)

T4R3

3

3

0

0

1

3

3

S(3), 60%(3)

S(3), 60%(3)

T4R4

3

3

0

0

1

3

3

S(3), 60%(3)

S(3), 60%(3)

T4R5

3

3

0

0

0

3

3

S(3), 60%(3)

S(3), 60%(3)

T4R6

3

3

0

0

1

3

3

S(3), 60%(3)

S(3), 60%(3)

T4R7

2

2

0

0

1

2

2

S(2), 60%(2)

S(2), 60%(2)

T5R1

1

57.1

1

10

83.3

0

0

0

1

1

S(1), 95%(1)

S(1), 95%(1)

T5R2

1

0

0

0

0

1

1

S(1), 95%(1)

D(1), 100%(1)

T5R3

3

3

0

0

0

3

3

S(3), 95%(3)

S(3), 95%(3)

T5R4

3

3

0

0

0

3

3

S(3), 95%(3)

S(3), 95%(3)

T5R5

1

0

0

0

0

1

1

S(1), 95%(1)

D(1), 100%(1)

T5R6

2

2

0

0

1

2

2

S(2), 95%(2)

S(2), 95%(2)

T5R7

1

1

0

0

0

1

1

S(1), 90%(1)

S(1), 90%(1)

Note: *: 7 and 14 days after 50% emergence of the seedlings in the control(s) group; S:Stunted growth; D: Dead.

contd. Soya DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

 

Group & Replicate

Shoot height on day 14

Shoot weight on day 14

Dry shoot weight (mg) on day 14

% Shoot height reduction on day 14

Dry shoot weight (g) on day 14

Average dry shoot weight (g) on day 14

% Dry shoot weight reduction on day 14

1

2

3

Average

04-Oct

05-Oct

T3R1

20.6

21.0

21.2

20.9

0.5250

525.0

-27.1*

3.5653

0.1783

-24.1

T3R2

20.8

22.8

22.5

22.0

0.5428

542.8

T3R3

21.1

22.0

23.3

22.1

0.5446

544.6

T3R4

19.4

20.3

20.9

20.2

0.4974

497.4

T3R5

21.0

23.1

22.8

22.3

0.5490

549.0

T3R6

22.0

22.7

-

22.4

0.3632

363.2

T3R7

22.8

21.6

22.2

22.2

0.5433

543.3

T4R1

12.3

12.5

12.6

12.5

0.3076

307.6

-56.8*

1.9911

0.1048

-55.4*

T4R2

12.4

13.6

-

13.0

0.2000

200.0

T4R3

12.6

13.1

13.9

13.2

0.3248

324.8

T4R4

11.6

12.1

12.4

12.0

0.2964

296.4

T4R5

12.5

13.7

13.6

13.3

0.3271

327.1

T4R6

13.1

13.5

12.3

13.0

0.3195

319.5

T4R7

13.6

12.9

-

13.3

0.2157

215.7

T5R1

1.3

-

-

1.3

0.0209

20.9

-94.4*

0.2661

0.0266

-88.7*

T5R2

-

-

-

-

-

-

T5R3

1.5

1.2

1.9

1.5

0.0777

77.7

T5R4

1.6

1.9

1.9

1.8

0.0879

87.9

T5R5

-

-

-

-

-

-

T5R6

1.1

2.0

-

1.6

0.0506

50.6

T5R7

2.2

-

-

2.2

0.0290

29.0

*: Statistically significant difference in shoot height and dry shoot weight as compared with the vehicle control.

TABLE 1.            Sunflower DT - Seedling Emergence, Seedling Survival, Shoot Height, and Dry Shoot Weight[c1] [r2] 

Group & Replicate

Total no. of seeds emerged

% of Seedling emergence

Seedlings survival on day 14

Total no. of seedlings survival on day 14

% of Seedling survival

18-Sep

19-Sep

20-Sep

Seedling emergence

Plant observations

*Day 7

*Day 14

*Day 7

*Day 14

27-Sep

04-Oct

27-Sep

04-Oct

CR1

3

100.0

3

21

100.0

0

0

2

3

3

N(3), 0%(3)

N(3), 0%(3)

CR2

3

3

0

0

2

3

3

N(3), 0%(3)

N(3), 0%(3)

CR3

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR4

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR5

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

CR6

3

3

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

CR7

3

3

0

0

2

3

3

N(3), 0%(3)

N(3), 0%(3)

VR1

3

95.2

3

20

100.0

0

0

1

3

3

N(3), 0%(3)

N(3), 0%(3)

VR2

2

2

0

0

2

2

2

N(2), 0%(2)

N(2), 0%(2)

VR3

3

3

0

0

2

3

3

N(3), 0%(3)

N(3), 0%(3)

VR4

3

3

0

0

2

3

3

N(3), 0%(3)

N(3), 0%(3)

VR5

3

3

0

0

3

3

3

N(3), 0%(3)

N(3), 0%(3)

VR6

3

3

0

0

2

3

3

N(3), 0%(3)

N(3), 0%(3)

VR7

3

3

0

0