2,4-dichloro-1-(trichloromethyl)benzene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1989-02-28 to 1989-04-07

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2,4-Dichlorobenzotrichloride

IUPAC Name:

2,4-Dichlorobenzotrichloride

Constituent 2

Reference substance name:

2,4-dichloro-1-(trichloromethyl)benzene

EC Number:

235-868-1

EC Name:

2,4-dichloro-1-(trichloromethyl)benzene

Cas Number:

13014-18-1

Molecular formula:

C7H3Cl5

IUPAC Name:

2,4-dichloro-1-(trichloromethyl)benzene

Test material form:

solid: crystalline

Details on test material:

Please refer to confidential details on test material

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Details on test solutions:

Due to the pure solubility the test item was dissolved in a water-Tween 80 solution (0.095 ml/L) . The test item hydrolysed completly to 2,4-dichlorobenzoic acid within 2h. All results are related to this degradation product.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

Strain: Hamilton-Buchanan
- Source: West Aquarium Bad Lauterberg, Germany
- Age at study initiation: 3 months
- Length at study initiation (mean, range and SD): 3.0 cm, 2.5 - 3.5 cm, 0.30 cm
- Method of breeding: 22 +- 1 °C; O2-content >= 80% ; 12 h day/night cycle, 700 Lux; <= 1g fish/l water;
- Feeding during test: no feeding during test

ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions: same as test
- Food type: Tetra Min, Tetra Werke, Melle, Germany;
- Amount: ad libitum;
- Frequency: 2 times a day;

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Remarks on exposure duration:

Observation times: 2-4, 24, 48, 72 and 96 hours

Test conditions

Test temperature:

Test groups: 21.6 - 23.0 °C
Control groups: 21.0 - 21.5 °C

pH:

Test groups: 7.2 - 7.9
Control groups: 7.6 - 8.2

Dissolved oxygen:

Test groups: 4.0 - 9.0 mg/L
Control groups: 6.5 - 9.5 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Effect concentrations 3

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

50 - 100 mg/L

Nominal / measured:

nominal

Effect concentrations 4

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

50 - 100 mg/L

Nominal / measured:

nominal

Effect concentrations 5

Duration:

48 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Effect concentrations 6

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Applicant's summary and conclusion

Conclusions:

Under the conditions of the present study conducted with 2,4-Dichlorobenzotrichloride the LC0 (48 h and 96 h), LC50 (48 h and 96 h) and LC100 (48h and 96h) was determined to be 22 mg/L, 50 - 100 mg/L and 100 mg/L, respectively.

According to Regulation (EC) No 1272/2008 (CLP) 2,4-Dichlorobenzotrichloride should be classified as aquatic chronic 3, H412 (harmful to aquatic life with long lasting effects).