1,4-bis(mesitylamino)anthraquinone

Endpoint summary

Additional information

In the acute immobilization test with Daphnia magna (STRAUS) the effects of the saturated solution of the test item ( nominal 100 mg/L) was determined according to OECD 202 (2004) and Directive 92/69/EEC Method C (1992). The limit test was conducted under static conditions over a period of 48 h. 20 test organisms were exposed to the saturated solution and the control. The solubility of the test item is significantly lower than 1 mg/L and probably in the range of 1 to 20 µg/L and therefore an analytical determination was not done. The validity criteria of the test guideline were fulfilled. At the saturated solution of the test item no biologically significant effect was determined.

The toxicity of the test item to fish (rainbow trout) was tested in replicate (10 fish per test) with nominal 1.0 mg/L. After 96 hours no mortality was observed. Test concentrations in excess of 1.0 mg/l could not be prepared due to the limited solubility of the test material in water.Therefore, at the saturated solution of the test item no biologically significant effect was determined.

The Daphnia magna Reproduction Test (Semi-Static, 21 d) of the test item was conducted as a limit test with the saturated solution* with 1.00 mg/L according to OECD 211.

Test species wasDaphnia magnaSTRAUS (Clone 5). Ten daphnids individually held were used for the saturated solution* and the control. At the start of the exposure, the daphnids were 2 to 24 h old. The test method was semi-static with renewal of the test solutions three times per week. The aim of the Daphnia Reproduction Test over 21 days was to assess effects on the reproduction capacity and other test item-related effects on parameters such as intrinsic rate of natural increase, occurrence of aborted eggs and stillborn juveniles, time of production of the first brood, adult mortality, dry body weight and length of the parental daphnids.

A specific analytic of the test item was not possible because of the low solubility of 0.02 mg/L of the test item in water. The results given are based on the nominal concentration of 1.00 mg/L of the test item in the saturated solution*.

·  The averagenumberofjuveniles per parental daphnidwere 84 for the control and 83 for the saturated test item solution* with 1.00 mg test item/L after 21 days. The reproductive output of daphnids in the saturated test item solution* (limit concentration) was comparable to the output of daphnids in the control(t-test,p = 0.05).

·  The coefficient of variation of thenumber of living offspringproduced per parent was 5 % for the control and 9 % for thesaturated test item solution*.

·  Theintrinsic rates of natural increase (IR)of the surviving parent daphnids accounting for generation time and number of offspring were calculated as a measure for population growth and maintenance. The IR of thesaturated solution*was comparabletothe control group (t-test,p = 0.05).

·  Thefirst broodwas released from all parent daphnids in all treatments until day 9. Four broods were observed during the test period for all parental daphnids.

·  Nodead juveniles or aborted eggswere observed in the control and the saturated solution.

·  Noadult mortalityor immobilization of parental daphnids was observedin the control andthesaturated solution*.

·  The meandrybody weightandtotal body lengthsof all parental daphnids of the saturated solution and the control were determined at the end of the study.
The mean dry body weight of the parental daphnids was 1.05 mg per daphnid for the control and 1.03 mg per daphnid for the saturated test item solution.
Themean bodylength of the parental daphnids was 5.50 mm per daphnid for the control and 5.33 mm for the saturated test item solution.

·  Nomalesandephippia(winter eggs) were observed in the control or test groups

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At the saturated test item solution with 1.00 mg/L of test item no biologically or statistically significant effects were determined. The No Observed Effect Concentration (NOEC) and the Lowest Observed Effect Concentration (LOEC) were as follows: NOEC: 1.00 mg/L (saturated solution) LOEC:> 1.00 mg/L (saturated solution) All provided effect values are based on the nominal loading rate of the test item.

An activated sludge respiration inhibition test was performed with the test item using a mixed population of activated sewage sludge microorganisms from the aeration Stage of Severn Trent Water Authority Sewage Treatment Plant, Belper, Derbyshire. The sample was maintained at 21°C with continuous aeration and used on the day of collection. The pH was buffered to pH 7.0 with sodium hydroxide solution and the suspended solids level adjusted to 3.8 g/l prior to use. As reference substance 3,5 dichlorophenol was used.

The oxygene-consumption rates were measured after 3 hours contact time and the percentage of inhibition was determined. For the test substance three tests were performed with a concentration of 1000 mg/L each resulting in 1, -7 and -7 % inhibition. Testing the reference substance 3,5 -dichlorophenol resulted in 87% inhibition for a concentration of 32 mg/L.

Based on the test the EC50 value for the toxicity of the test item to bacteria was determined as > 1000 mg/L and the NOEC is 1000 mg/L.