1,4-bis(mesitylamino)anthraquinone

Long-term toxicity to aquatic invertebrates

Description of key information

At the saturated test item solution with 1.00 mg/L of test item no biologically or statistically significant effects were determined. The No Observed Effect Concentration (NOEC) and the Lowest Observed Effect Concentration (LOEC) were as follows: NOEC: 1.00 mg/L (saturated solution) LOEC:> 1.00 mg/L (saturated solution) All provided effect values are based on the nominal loading rate of the test item.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 mg/L

Additional information

TheDaphnia magnaReproduction Test (Semi-Static, 21 d) of the test item was conducted as a limit test with the saturated solution* with 1.00 mg/L according to OECD 211.

Test species wasDaphnia magnaSTRAUS (Clone 5). Ten daphnids individually held were used for the saturated solution* and the control. At the start of the exposure, the daphnids were 2 to 24 h old. The test method was semi-static with renewal of the test solutions three times per week. The aim of the Daphnia Reproduction Test over 21 days was to assess effects on the reproduction capacity and other test item-related effects on parameters such as intrinsic rate of natural increase, occurrence of aborted eggs and stillborn juveniles, time of production of the first brood, adult mortality, dry body weight and length of the parental daphnids.

A specific analytic of the test item was not possible because of the low solubility of 0.02 mg/L of the test item in water. The results given are based on the nominal concentration of 1.00 mg/L of the test item in the saturated solution*.

·  The averagenumberofjuveniles per parental daphnidwere 84 for the control and 83 for the saturated test item solution* with 1.00 mg test item/L after 21 days. The reproductive output of daphnids in the saturated test item solution* (limit concentration) was comparable to the output of daphnids in the control(t-test,p = 0.05).

·  The coefficient of variation of thenumber of living offspringproduced per parent was 5 % for the control and 9 % for thesaturated test item solution*.

·  Theintrinsic rates of natural increase (IR)of the surviving parent daphnids accounting for generation time and number of offspring were calculated as a measure for population growth and maintenance. The IR of thesaturated solution*was comparabletothe control group (t-test,p = 0.05).

·  Thefirst broodwas released from all parent daphnids in all treatments until day 9. Four broods were observed during the test period for all parental daphnids.

·  Nodead juveniles or aborted eggswere observed in the control and the saturated solution.

·  Noadult mortalityor immobilization of parental daphnids was observedin the control andthesaturated solution*.

·  The meandrybody weightandtotal body lengthsof all parental daphnids of the saturated solution and the control were determined at the end of the study.
The mean dry body weight of the parental daphnids was 1.05 mg per daphnid for the control and 1.03 mg per daphnid for the saturated test item solution.
Themean bodylength of the parental daphnids was 5.50 mm per daphnid for the control and 5.33 mm for the saturated test item solution.

·  Nomalesandephippia(winter eggs) were observed in the control or test groups

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At the saturated test item solution with 1.00 mg/L of test item no biologically or statistically significant effects were determined. The No Observed Effect Concentration (NOEC) and the Lowest Observed Effect Concentration (LOEC) were as follows: NOEC: 1.00 mg/L (saturated solution) LOEC:> 1.00 mg/L (saturated solution) All provided effect values are based on the nominal loading rate of the test item.