2-(propyloxy)ethanol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1987

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was conducted according to guideline and/or standard method but was there were no data on GLPs.

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Methods for Acute Toxicity Tests with Fish Macroinvertebrates and Amphibians, Ecological Research Series, EPA-600/3-75-009 , April, 1975.

Deviations:

yes

Remarks:

Replicate concentrations are not routinely used.

Principles of method if other than guideline:

Not applicable

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-(propyloxy)ethanol

EC Number:

220-548-6

EC Name:

2-(propyloxy)ethanol

Cas Number:

2807-30-9

Molecular formula:

C5H12O2

IUPAC Name:

2-(propyloxy)ethanol

Details on test material:

Test material: Propyl CELLOSOLVE
Source: Union Carbide Corporation

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

Dissolved oxygen and pH were determined initially and at 48 hours for all test concentrations and controls.
Mortalities were recorded at 24 and 48 hours.

Test solutions

Vehicle:

not specified

Details on test solutions:

Kanawha River water obtained from the South Side Boat Ramp (Charleston, at C&P Telephone Company Building) was used in preparation of test solutions. This water is soft and its quality is sufficiently high that it can be used for maintaining long-term Daphnia cultures. The following analyses were obtained on the water:
Total Hardness 60 mg/L as CaCO3
Total Alkalinity 38 mg/L as CaCO3
pH 7.1
Conductivity 250 umhos/cm

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna used in the bioassay came from a culture maintained in the laboratory for several years. This culture was initially stocked from the
EPA laboratory at Duluth. MN. The culture was maintained at 19-23 Deg. C in glass beakers filled with Kanawha River water. Culture transfers and feeding were performed three times a week. The transfer consists of placing 20-30 young Daphnia into each of three 600 ml beakers of river water. The
laboratory-prepared food consisted of pulverized trout food, yeast and alfalfa powder.

The Daphnia neonates ( first instars) used in testing were less than 24 hours old, and were obtained by isolating gravid females for approximately 20 hours.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Test conditions

Hardness:

60 mg/L as CaCO3

Test temperature:

No data

pH:

7.1

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

5-10 geometrically equidistant concentrations plus control

Details on test conditions:

The definitive test was designed to provide a series of from 5-10 geometrically equidistant concentrations plus a control .
The test was conducted in 250 mL beakers containing 200 mL of the test solution and 10 Daphnia. The Daphnia neonates ( first instars) used in testing were less than 24 hours old, and were obtained by isolating gravid females for approximately 20 hours.
Dissolved oxygen and pH were determined initially and at 48 hours for all test concentrations and controls.
Mortalities were recorded at 24 and 48 hours.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 5 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Details on results:

No additional data

Results with reference substance (positive control):

No data

Reported statistics and error estimates:

The Trimmed Spearman-Karber computer program was used to calculate the 48-hour LC50 and 95 percent confidence intervals

Any other information on results incl. tables

None

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The 48h LC50 to Daphnia magna is >5000 mg/L.

Executive summary:

48 hour Static definitive tests were conducted by EPA-ASTM procedures.

The definitive test was designed to provide a series of from 5-10 geometrically equidistant concentrations plus a control. The test was conducted in 250 mL beakers containing 200 mL of the test solution and 10 Daphnia. The Daphnia neonates (first instars) used in testing were less than 24 hours old, and were obtained by isolating gravid females for approximately 20 hours. Daphnia magna used in the bioassay came from a culture maintained in the laboratory for several years. This culture was initially stocked from the EPA laboratory at. MN.

Dissolved oxygen and pH were determined initially and at 48 hours for all test concentrations and controls.

Mortalities were recorded at 24 and 48 hours. The Trimmed Spearman-Karber computer program was used to calculate the 48-hour LC50 and 95 percent confidence intervals.

The 48h LC50 value to Daphnia magna was found to be >5000 mg/L.