Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 627-725-0 | CAS number: 398489-26-4
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- April 2013 - July 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: This study has been performed according to OECD and/or EC guidelines and according to GLP principles.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 013
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- yes
- Remarks:
- The abiotic sterile control was prepared with deionised water and not with medium. This procedure, according to standard operating procedures, is chosen to reduce the possibility of any microbial activity. It was wrongly indicated in the study plan.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tert-butyl 3-oxoazetidine-1-carboxylate
- EC Number:
- 627-725-0
- Cas Number:
- 398489-26-4
- Molecular formula:
- C8H13NO3
- IUPAC Name:
- Tert-butyl 3-oxoazetidine-1-carboxylate
- Test material form:
- solid: particulate/powder
- Remarks:
- migrated information: powder
- Details on test material:
- Identification PAM-Azetidinone
Chemical name 3-Oxoazetidine-1-carboxylic acid tert-butyl ester
Batch no RC10050008
CAS no. 398489-26-4
Molecular formula C8H13NO3
BMG sample no. M1302-00844-01
Physical form Solid
Purity 99.4% (w/w)
Theoretical oxygen demand 2.056 mg O2/mg (calculated according to chapter 5.2 assuming nitrification)
Test substance storage At room temperature, protected from light
Stability Stable under storage conditions
Expiry date 22 June 2013
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Duration of test (contact time):
- ca. 28
Initial test substance concentration
- Initial conc.:
- ca. 100 mg/L
- Based on:
- ThOD/L
Results and discussion
% Degradation
- Parameter:
- % degradation (O2 consumption)
- Value:
- ca. 15
- Sampling time:
- 28 d
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- PAM-Azetidinone is not biodegradable in the Manometric Respirometry Test.
- Executive summary:
The biodegradability of PAM-Azetidinone exposed to microorganisms derived from activated sludge of a municipal sewage treatment plant was investigated under aerobic static exposure conditions.
No significant biodegradability (15%) of PAM-Azetidinone based on O2 consumption was observed during 28 days as compared to the theoretical oxygen demand (ThOD).
The procedure control sodium benzoate reached 80% biodegradation after 14 days, thus confirming suitability of inoculum and test conditions.
The calculated biodegradation based on DOC measurements reached 19% for PAM-Azetidinone and 99% for sodium benzoate, respectively. The data confirm that no significant biodegradability occurred.
PAM-Azetidinone is not biodegradable in the Manometric Respirometry Test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
