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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
31st August 1995
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions. This study was performed in compliance with the GLP regulations. Only memorandum available; no full study report.

Data source

Reference
Reference Type:
other company data
Title:
Unnamed
Year:
1995
Report date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
not specified
Qualifier:
according to guideline
Guideline:
EU Method C.3 (Algal Inhibition test)
Deviations:
not specified
Principles of method if other than guideline:
No further details were provided.
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
PTH-Decahydroamide
IUPAC Name:
PTH-Decahydroamide
Constituent 2
Reference substance name:
Ro 31-9373/000
IUPAC Name:
Ro 31-9373/000
Details on test material:
No further details were provided.
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No further details were provided.

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
No further details were provided.

Test solutions

Vehicle:
not specified
Details on test solutions:
No further details were provided.

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
Details on test organisms:
No further details were provided.

Study design

Test type:
not specified
Water media type:
not specified
Remarks on exposure duration:
No further details were provided.
Post exposure observation period:
No further details were provided.

Test conditions

Hardness:
No further details were provided.
Test temperature:
No further details were provided.
pH:
No further details were provided.
Dissolved oxygen:
No further details were provided.
Salinity:
No further details were provided.
Nominal and measured concentrations:
No further details were provided.
Details on test conditions:
No further details were provided.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
4.8 mg/L
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
46 mg/L
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
74 mg/L
Basis for effect:
growth rate
Details on results:
Since this concentration was below the detection limit of PTH-decahydroamide (Ro 31-9373/000) in water samples, no actual concentration could be determined.
Results with reference substance (positive control):
No further details were provided.
Reported statistics and error estimates:
No further details were provided.

Any other information on results incl. tables

No further details were provided.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
PTH-Decahydroamide (Ro 31-9373/000) inhibited cell growth and reduced growth rate of Scenedesmus subspicatus significantly under the conditions of the present study at nominally 10 mg/l and higher. The inhibition rates were estimated for both the algal biomass b and the growth rate R after 72 hours test duration; based on nominal concentrations.
EbC50 (0-72 h)                     46 mg/l
ERC50 (0-72 h)                     74 mg/l
The NOEC was 4.8 mg/l for both cell growth inhibition and growth rate reduction when based on nominal concentrations. Since this concentration was below the detection limit of PTH-decahydroamide (Ro 31-9373/000) in water samples, no actual concentration could be determined.
Executive summary:

The study procedure described was performed on the basis of EC directive 92/69/EEC, C. 3 (1992) and OECD guideline No. 201 (1984).

PTH-Decahydroamide (Ro 31-9373/000) inhibited cell growth and reduced growth rate of Scenedesmus subspicatus significantly under the conditions of the present study at nominally 10 mg/l and higher. The inhibition rates were estimated for both the algal biomass b and the growth rate R after 72 hours test duration; based on nominal concentrations.

EbC50 (0-72 h)                     46 mg/l

ERC50 (0-72 h)                     74 mg/l

The NOEC was 4.8 mg/l for both cell growth inhibition and growth rate reduction when based on nominal concentrations. Since this concentration was below the detection limit of PTH-decahydroamide (Ro 31-9373/000) in water samples, no actual concentration could be determined.