Butanamide, N-(3-ethoxypropyl)-2,4-dihydroxy-3,3-dimethyl-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 1994-09-14 to 1994-11-16

Reliability:

1 (reliable without restriction)

Cross-reference

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Report date:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

Directive 92/69/EEC

Deviations:

no

Principles of method if other than guideline:

NA

GLP compliance:

yes

Test material

Test material information

Constituent 1

Test material form:

liquid: viscous

Details on test material:

NA

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
NA

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Frequency: at t=0h and t=48h
Volume: 10 mL from the approximate centre of the test vessel

Test solutions

Vehicle:

yes

Details on test solutions:

The test solution, concentration 100 mg/L, was prepared by adding quantitatively 24.8 mg test substance to 250 mL ISO medium. The final test solution was clear without precipitation.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

For the test selection of young daphnia with an age of < 24 hours.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

NA

Test conditions

Hardness:

250 mg CaCO3/L

Test temperature:

The temperature measured in blank control was 21 °C.

pH:

8.0 +/- 0.2

Dissolved oxygen:

between 8.5 and 9.1 mg O2/L

Salinity:

NA

Nominal and measured concentrations:

Analysis of the sample taken at the start (t=0) of the final test revealed an actual concentration of 113 mg/L (113%) at the nominal concentration of 100 mg/L. During the exposure period the actual concentration remained above 80 % of the initial.

Details on test conditions:

NA

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

NA

Results with reference substance (positive control):

Concentrations: 0.00, 0.10, 0.18, 0.32, 0.56 and 1.0 mg/L in ISO-medium (without test substance)
The EC50 after 24 and 48 hours was between 1.0 and 1.8 mg/L.

Reported statistics and error estimates:

NA

Any other information on results incl. tables

no remarks

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC50 > 100 mg/L

Executive summary:

The 48h-EC50 of the test item for immobilization of daphnia was greater than 100 mg/L, the maximum concentration to be tested.