[No public or meaningful name is available]

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

benzene, 1,4-bis[2-[3,5-dimethoxy-4-(1- methylpropoxy)phenyl]ethenyl]-

Cas Number:

586410-55-1

Molecular formula:

C34 H42 O6

IUPAC Name:

benzene, 1,4-bis[2-[3,5-dimethoxy-4-(1- methylpropoxy)phenyl]ethenyl]-

Details on test material:

Read-across from CASRN586410-55-1 to the substance defined in section 1 allowed by belgian authority.
The study consisted of six bottles:
- 2 blank controls (no test material)
- 2 test bottles (test substance, 16mg/l)
- 1 positive control (sodium acetate, 40mg/l)
- 1 toxicity control (test substance, 16mg/l; plus sodium acetate, 40mg/l)

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

16 mg/L

Based on:

other: organic carbon content

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

other: Sodium acetate

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

ca. 0

Sampling time:

28 d

Details on results:

Points of degradation plot (test substance):
0 % degradation after 2 d
0 % degradation after 5 d
0 % degradation after 7 d
0 % degradation after 9 d
0 % degradation after 14 d
0 % degradation after 19 d
0 % degradation after 23 d
0 % degradation after 27 d
0 % degradation after 29 d

BOD5 / COD results

Results with reference substance:

Points of degradation plot (reference substance):
16 % degradation after 2 d
42 % degradation after 5 d
60 % degradation after 7 d
70 % degradation after 9 d
76 % degradation after 14 d
81 % degradation after 19 d
82 % degradation after 23 d
84 % degradation after 27 d
84 % degradation after 29 d

Any other information on results incl. tables

1. theoretical CO2 production:

- The theoretical CO2 production of the test substance was calculated to be 2.74mg CO2/mg.

2. biodegradation:

- temperature: 21.1°C - 22.7°C

- pH of the different test media: 7.5 -7.6 (just before the start of the test) up to 7.6 -8.1 (on day 28)

- In the toxicity control more than 25% degradation occurred within 14 days (36%, based on ThCO2). Therefore, the test substance was assumed to be not inhibitory to microbial activity.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

- not readily biodegradable
- not to inhibit microbial activity
Due to the high log Pow (>4), the low water solubility (<1mg/l), the 48EC50 and 72LC50 which might both be >100mg/l and the non-readily biodegradability, the substance should be classified for 'chronic aquatic toxicity' (DSD: R53, CLP: cat.4).