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Diss Factsheets
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EC number: 208-759-1 | CAS number: 540-84-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Endpoint summary
Administrative data
Description of key information
Skin sensitisation
Based on available read across data (Magnusson and Kligman Guinea-Pig Maximization tests (OECD TG 406)), 2,2,4 -trimethylpentane is not considered to be a skin sensitiser.
Respiratory sensitisation
No data available
Key value for chemical safety assessment
Skin sensitisation
Link to relevant study records
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Basic data given
- Justification for type of information:
- A discussion and report on the read across strategy is given as an attachment in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 406 (Skin Sensitisation)
- GLP compliance:
- no
- Type of study:
- guinea pig maximisation test
- Justification for non-LLNA method:
- Study was conducted prior to implementation of the LLNA method.
- Species:
- guinea pig
- Strain:
- other: p-strain
- Sex:
- male/female
- Route:
- intradermal and epicutaneous
- Vehicle:
- corn oil
- Concentration / amount:
- 1 % w/v in corn oil for intradermal induction
50 % for topical induction
25 % for topical challenge - Route:
- epicutaneous, occlusive
- Vehicle:
- corn oil
- Concentration / amount:
- 1 % w/v in corn oil for intradermal induction
50 % for topical induction
25 % for topical challenge - No. of animals per dose:
- 20 (10 males, 10 females), controls: 10 (5 males, 5 females)
- Positive control substance(s):
- no
- Key result
- Group:
- negative control
- Remarks on result:
- no indication of skin sensitisation
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 0
- Group:
- test chemical
- Dose level:
- 25 % w/v in corn oil
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- no skin sensitization
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 0.0. Group: test group. Dose level: 25 % w/v in corn oil. No with. + reactions: 0.0. Total no. in groups: 20.0. Clinical observations: no skin sensitization.
- Key result
- Reading:
- 2nd reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 25 % w/v in corn oil
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- no skin sensitization
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 24.0. Group: test group. Dose level: 25 % w/v in corn oil. No with. + reactions: 0.0. Total no. in groups: 20.0. Clinical observations: no skin sensitization.
- Key result
- Reading:
- other: 3rd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 25 % w/v in corn oil
- No. with + reactions:
- 0
- Total no. in group:
- 20
- Clinical observations:
- no skin sensitization
- Remarks on result:
- other: Reading: other: 3rd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 25 % w/v in corn oil. No with. + reactions: 0.0. Total no. in groups: 20.0. Clinical observations: no skin sensitization.
- Key result
- Group:
- positive control
- Remarks on result:
- not measured/tested
- Interpretation of results:
- other: not sensitising
- Conclusions:
- The purpose of this study was to determine the skin sensitization potential of the test substance, hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics. Ten male and ten female guinea pigs were used as a test group, and five male and five female guinea pigs were used as a control group. The test group underwent an intradermal induction of 1.0 %w/v test material in corn oil. Then a topical induction of 50% w/v test substance in corn oil. The challenge was done with 25% w/v test material in corn oil. No skin reactions were noted during the challenge. The test substance is not sensitizing.
- Executive summary:
The purpose of this study was to determine the skin sensitization potential of the test substance, hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics. Ten male and ten female guinea pigs were used as a test group, and five male and five female guinea pigs were used as a control group. The test group underwent an intradermal induction of 1.0 %w/v test material in corn oil. Then a topical induction of 50% w/v test substance in corn oil. The challenge was done with 25% w/v test material in corn oil. No skin reactions were noted during the challenge. The test substance is not sensitizing.
- Endpoint:
- skin sensitisation: in vitro
- Data waiving:
- study scientifically not necessary / other information available
- Justification for data waiving:
- an in vitro skin sensitisation study does not need to be conducted because adequate data from an in vivo skin sensitisation study are available
Referenceopen allclose all
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not sensitising)
- Additional information:
There is no skin sensitisation data available for 2,2,4 -trimethylpentane. However, data is available for structural analogue, Hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics and presented in the dossier. This data is read across to 2,2,4-trimethylpentane based on analogue read across and a discussion and report on the read across strategy is provided as an attachment in IUCLID Section 13.
Hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics
The skin sensitization potential of hydrocarbons, C7-C9, n-alkanes, isoalkanes, cyclics, was studied in a guinea pig maximization test (Magnusson/Kligman method) similar to OECD 406 (Shell Chemicals, 1977). Guinea pigs (10/sex in treated group and 5/sex in control group) were induced with 1% (w/v) of the test substance in corn oil as a 0.1 ml injection in the shaved shoulder region. Six to eight days later a topical induction of 50% (w/v) test material was applied under an occlusive dressing for 48 hrs. On Day 20-21 guinea pigs were challenged with 25% (w/v) test material applied to the shaved flank. Skin response was evaluated immediately after challenge, and 24 and 48 hours later. The test substance did not cause indication of skin sensitization in any guinea pig at any evaluation period after challenge.
Respiratory sensitisation
Endpoint conclusion
- Endpoint conclusion:
- no study available
- Additional information:
There are no reports of respiratory sensitisation from 2,2,4-trimethylpentane in laboratory animals or in humans. However, the read across skin sensitisation study found no indication of skin sensitisation in guinea pigs. With these observations, it is presumed that 2,2,4-trimethylpentane will not be a respiratory sensitising agent.
Justification for classification or non-classification
Based on available read across data, 2,2,4-trimethylpentane does not meet the criteria for classification as a skin or respiratory sensitiser under the new Regulation (EC) 1272/2008 on classification, labeling and packaging of substances and mixtures (CLP).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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