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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 221-761-7 | CAS number: 3228-02-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Long-term toxicity to fish
Administrative data
- Endpoint:
- fish short-term toxicity test on embryo and sac-fry stages
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- No data
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- Study conducted according to OECD Guideline 212 with deviations: details of feeding, water quality characteristics, light and temperature, test concentrations, pH, dissolved oxygen concentration, individual/summary results not reported however, it is stated that the studes were performed in conformtiy with the Guideline thus the quality criteria should have been met in this study. GLP status not reported and study performed after 2008 so not officially eligible as a valid test under REACH Regulation
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- publication
- Title:
- Ecotoxicity and screening level ecotoxicological risk assessment of five antimicrobial agents: triclosan, triclocarban, resorcinol, phenoxyethanol and p-thymol.
- Author:
- Tamura I, Kagota K, Yasuda Y, Yoneda S, Morita J, Nakada N, Kameda Y, Kimura K, Tatarazako N and Yamamoto H.
- Year:
- 2 013
- Bibliographic source:
- J Appl Toxicol. 33 (11):1222-1229.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 212 (Fish, Short-term Toxicity Test on Embryo and Sac-Fry Stages)
- Deviations:
- yes
- Remarks:
- details of feeding, water quality characteristics, light and temperature, test concentrations, pH, dissolved oxygen concentration, individual/summary results not reported
- Principles of method if other than guideline:
- Not applicable
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 4-isopropyl-m-cresol
- EC Number:
- 221-761-7
- EC Name:
- 4-isopropyl-m-cresol
- Cas Number:
- 3228-02-2
- Molecular formula:
- C10H14O
- IUPAC Name:
- 4-isopropyl-m-cresol
- Test material form:
- not specified
- Details on test material:
- - Name of test material (as cited in study report): p-thymol
- Source: Wako Pure Chemical Co. (Osaka, Japan)
- Analytical purity: 99%
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility: 860 mg/L
- log Kow:: 3.52
- pKa: 10.36 ± 0.18
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebra fish
Study design
- Test type:
- semi-static
- Water media type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 9 d
- Post exposure observation period:
- None
Test conditions
- Hardness:
- No data
- Test temperature:
- No data
- pH:
- pH was monitored at the replacement of the solutions.
- Dissolved oxygen:
- Dissolved oxygen was monitored at the replacement of the solutions.
- Salinity:
- No data
- Nominal and measured concentrations:
- No data
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL beaker
- Renewal rate of test solution: The solutions were replaced every 48 h
- No. of fertilized eggs/embryos per vessel: 15 embryos per vessel
- No. of vessels per concentration (replicates): 3
EFFECT PARAMETERS MEASURED
The percentage hatching and the survival rate of the larvae were evaluated until the egg yolk had disappeared. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 9 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.5 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: hatching and the survival of larvae
- Reported statistics and error estimates:
- The NOEC for hatching and the survival of larvae was determined using the probit or logit conversion with Ecotox-Statics version 2.6d (Yoshioka, 2001) distributed by the Japan Society for Environmental Toxicology.
Again, the geometrical mean of the measured concentration of the test item was used to determine NOEC.
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the test conditions, NOEC for hatching and the survival of larvae was 1.5 mg/L based on the geometric means of the exposure concentrations.
- Executive summary:
In a short-term toxicity test on embryo and sac-fry stages performed according to OECD guideline 212, the juvenile larvae of Zebra fish (Danio rerio) were exposed to test item for 9 days.
At least three replicates of 15 embryos were placed in 50 ml beaker for five concentrations. The solutions were replaced every 48 h (i.e. a semi-static system) and the concentration of the test item was monitored using the HPLC. The percentage hatching and the survival rate of the larvae were evaluated until the egg yolk had disappeared. The NOEC for hatching and the survival of larvae was determined using the probit or logit conversion with Ecotox-Statics version 2.6d (Yoshioka, 2001) distributed by the Japan Society for Environmental Toxicology.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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