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EC number: 233-469-7 | CAS number: 10192-30-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Data waiving:
- other justification
- Justification for data waiving:
- other:
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- The study is comparable to the current OECD TG 207. However, the results are probably distorted due to interfering ammonia effects due to the test design.
- Qualifier:
- according to guideline
- Guideline:
- other: US EPA/600/3-88/029. (Earthworm survival)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- GLP compliance:
- not specified
- Remarks:
- not specified in publication
- Analytical monitoring:
- yes
- Details on sampling:
- Total and free ammonia were measured on days 1 and 7.
- Vehicle:
- no
- Details on preparation and application of test substrate:
- Stock solutions of NH4Cl were prepared on the test day or the previous day. Mixing into soil was not described.
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Source: Carolina Biological Supply (Burlington, NC)
- Age at test initiation: adult clitellate worms
- Weight at test initiation: 300-600 mg - Study type:
- laboratory study
- Substrate type:
- artificial soil
- Limit test:
- no
- Total exposure duration:
- 14 d
- Test temperature:
- 22 +/- 2 °C
- pH:
- 7.6
- Moisture:
- 40mL deionized water per 100 g dry soil
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): screw-top glass jars with one small nail hole punched in the lid for air exchange
- Amount of soil or substrate: 200 g dw
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE
- Composition (if artificial substrate): 70% 70-grade washed Silica Sand, 20% Kaolin Clay, 10% Canadian Peat Moss, CaCO3 for pH adjustment.
OTHER TEST CONDITIONS
- Photoperiod: constant light
- Light intensity: 400 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Mortality measured after 7 and 14 days. - Nominal and measured concentrations:
- Nominal: 0 (control), 100, 200, 300, 400, 500 mg NH4Cl/kg
Measured: Day 1: 0, 95.6, 191, 283, 371, 478 mg/kg total ammonia N
Day 7:Day 14: - Reference substance (positive control):
- yes
- Remarks:
- 2-chloroacetamid & KCl
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- 75 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: NH4 (ammonium)
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 54 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: NH4 (ammonium)
- Basis for effect:
- mortality
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- 223 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- NH4Cl
- Basis for effect:
- mortality
- Remarks on result:
- other: SD: 65 mg/kg
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 163 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- NH4Cl
- Basis for effect:
- mortality
- Remarks on result:
- other: SD: 35 mg/kg
- Details on results:
- - Nominal concentrations, with the average of the measured concentrations (for five concentrations), were at 95% of the nominal concentration.
- No control mortality was observed.- Results with reference substance (positive control):
- KCl:
7 day LC50: 6340 +/- 665 mg/kg soil dw
14 day LC50: 6030 +/- 689 mg/kg soil dw
2-Chloroacetamide:
7 day LC50: 33.3 +/- 12.1 mg/kg soil dw
14 day LC50: 33.1 +/- 11.7 mg/kg soil dw- Reported statistics and error estimates:
- LC50 with 95% Confidence Interval was calculated with trimmed Spearman-Karber Method.
- Validity criteria fulfilled:
- yes
- Remarks:
- control survival > 90%
- Conclusions:
- In a 14-d acute earthworm toxicity test according to US EPA/600/3-88/029, Eisenia fetida worms were exposed to ammonium chloride in artificial soil and effects on survival were assessed after 7 and 14 days. The 14 d LC50 for ammnonium chloride amounts to 163 mg/kg soil dw (nominal, analytically verified), which corresponds to a 14 d LC50 of 54 mg NH4/kg soil dw (nominal) for ammonium.
Referenceopen allclose all
Description of key information
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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