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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
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EC number: 233-469-7 | CAS number: 10192-30-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Neurotoxicity
Administrative data
- Endpoint:
- neurotoxicity
- Remarks:
- other: in-vitro
- Type of information:
- read-across based on grouping of substances (category approach)
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Short communication on sodium bisulphite toxicity to human neuroblastoma cells.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Toxicity of chloroprocaine and sodium bisulfite on human neuroblastoma cells.
- Author:
- Seravalli, E.; Lear, E.
- Year:
- 1 987
- Bibliographic source:
- Anesth. Analg. 66, 954-958
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Plates of human neuroblastoma cells were incubated with sodium bisulphite concentrations in the range from 0.08 to 0.8 mM at pH 7.4 for 3 or 20 hours. After the exposure, the medium was discarded and drug-free medium was added to the experimental and control cultures. The cultures were left undisturbed at 37˚C for 3, 6, and 9 days. Thereafter, the cultures were washed with saline and stained with Wright-Giemsa for cell counting. Cell multiplication was measured by the number of colonies that developed in each experimental and untreated culture.
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- Sodium hydrogensulfite
- EC Number:
- 231-548-0
- EC Name:
- Sodium hydrogensulfite
- Cas Number:
- 7631-90-5
- Molecular formula:
- NaHSO3
- IUPAC Name:
- sodium hydrogensulfite
- Details on test material:
- - Name of test material (as cited in study report): sodium bisulfite
- Molecular formula (if other than submission substance):NaHSO3
- Molecular weight (if other than submission substance): 104 g/mol
- Substance type: technical product
- Physical state: solid, white powder
- Other: sodium bisulfite was obtained from two commercial sources, hencefore identified as SB-1 and SB-2
No further details are given.
Constituent 1
Test animals
- Species:
- other: not applicable, in-vitro test
- Strain:
- other: not applicable, in-vitro test
- Details on test animals or test system and environmental conditions:
- not applicable, in-vitro test
Administration / exposure
- Route of administration:
- other: not applicable, in-vitro test
- Vehicle:
- other: not applicable, in-vitro test
- Details on exposure:
- - sodium bisulfite was diluted in serum-free medium (DMEM-vide supra)
- dilution were made as follows: SB concentrations ranging from 0.08 mM to 0.8 mM
- dilutions of all drugs were prepared immediately before addition to the cells - Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 3 or 20 hours
- Frequency of treatment:
- not applicable
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0.08 - 0.8 mM sodium bisulfite
Basis:
nominal conc.
- No. of animals per sex per dose:
- not applicable, in-vitro test
- Details on study design:
- - Dose selection rationale: the concentrations were chosen within limits that approximate the concentrations achieved in clinical practice
Examinations
- Statistics:
- Reduction of colony forming ability was determined by expressing the number of colonies in drug-treated cultures as a percentage of the number of colonies in its control culture. Inhibition was considered definite when it was ≥25 %. Student’s t-test (two-tailed) was used for statistical analysis. P values <0.05 were considered statistically significant.
Results and discussion
Results of examinations
- Clinical signs:
- not examined
- Mortality:
- not examined
- Body weight and weight changes:
- not examined
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Behaviour (functional findings):
- not examined
- Gross pathological findings:
- not examined
- Neuropathological findings:
- not examined
Effect levels
- Dose descriptor:
- NOAEL
- Remarks on result:
- not determinable
- Remarks:
- no NOAEL identified
Any other information on results incl. tables
- Sodium bisulphite reduced the cell multiplication in neuroblastoma cells.
- The colony-forming ability (CFA) was inhibited by 72% to 92% at a 3-h exposure to one commercial sample of sodium bisulphite and by 57% to 72% with another sample (both tested at concentrations of 0.8 mM).
- no difference in the inhibition of CFA was detected between both commercial samples at concentrations below 0.8 mM
- Prolongation of exposure time to 20 hours resulted in a CFA inhibition of 98% by both samples.
- cells exposed to either sample of sodium bisulfite for 20 hr did not resume ability to form colonies during the study period of 9 days
- The authors concluded that sodium bisulphite may have neurotoxicity of potential clinical significance because it is used as antioxidant in commercial solutions of chloroprocaine.
Applicant's summary and conclusion
- Conclusions:
- In this study sodium bisulfite was found to have an inhibitory effect on CFA of neuroblastoma that varied with concnetration and exposure time. Cell multiplication in human neuroblastoma cells was reduced by sodium bisulfite treatment (0.08-0.8 mM) for 3 or 20 hours.
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