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EC number: 911-811-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 7th September - 8th October 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Test material used was HEBMP (1-3Na). The active ingredient of HEBMP-xNa is the polyphosphonic acid form (HEBMP-H).
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: LUFA Standard soil 2.3
- Other:
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: The test item was weighed out and diluted in demineralized water for each test separately and applied to the surface of the soil. The soil was mixed with a mixer (approximately 3 minutes) to ensure a homogeneous distribution of the test item in the soil. Afterwards, the soil was distributed to the replicates.
VEHICLE: no vehicle used - Test organisms (inoculum):
- soil
- Total exposure duration:
- 28 d
- Test temperature:
- 20.0 - 21.5
- Moisture:
- 43.7 - 45.2 % moisture content
- Organic carbon content (% dry weight):
- 0.616
- Details on test conditions:
- TEST SYSTEM
- Testing facility: Noack Laboratorien GmbH, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany
- Test container (type, material, size): Plastic boxes (volume 1.0 L, food grade) with perforated tops to enable gas exchange. Incubation was performed in bulk and sub-samples were taken as stated below.
- Amount of soil: 400g soil dry weight per vessel
- No. of replicates per concentration: 3
- No. of replicates per control: 3
SOIL INCUBATION
- Method: bulk
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): Offenbach, “rechts der Landauer Str.“ Nr. 826/7, Rheinland-Pfalz, Germany. R-439683; H-5449554
- History of site: Cultures: 2017 - 2021: uncultivated. Fertilisation: 2017 - 2021: none
- Vegetation cover: not reported
- Treatments with pesticides or fertilizers: No crop protection products applied during the year of sampling and 4 preceding years. Fertilisation: 2017 - 2021: none
- Accidental contamination: not reported
- Other:
- Depth of sampling: ca. 0 - 20 cm
- Soil texture
- % sand: 57.6
- % silt: 35.1
- % clay: 7.4
- Soil taxonomic classification: Silty sand
- Soil classification system: German DIN classification
- pH (in water): 6.2 +/- 0.3
- Initial nitrate concentration for nitrogen transformation test (mg nitrate/kg dry weight): 6.55 mg/kg dry weight
- Maximum water holding capacity (in % dry weight): 42%
- Cation exchange capacity (mmol/kg): 6.0 +/- 0.9 meq / 100g
- Pretreatment of soil: The soil was manually cleared of large objects and then sieved to a particle size of up to 2 mm.
- Storage (condition, duration): The soil was stored for 2 days (2021-07-21 to 2021-07-23) in the dark at 4 - 6 °C in a climatic room.
- Initial microbial biomass as % of total organic C: 2.12
DETAILS OF PREINCUBATION OF SOIL (if any): The soil was pre-incubated at room temperature (ca. 20 °C) for 18 days (2021-07-23 to 2021-08-10) to adapt the micro-organisms to test conditions before the start of the study.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Inorganic nitrate concentration measured at 0, 7, 14 and 28 days. pH and water contents were determined on day 0 and 28
RANGE-FINDING STUDY
- Test concentrations: control, 10, 100 and 1000 mg active acid/kg dry weight
- Results used to determine the conditions for the definitive study:yes. No inhibition observed so proceeded with 1000 mg active acid/kg soil dry weight as highest concentration - Nominal and measured concentrations:
- Nominal: Control, 3.16, 9.83, 31.4, 99.4, 314 and 1000 mg active acid/kg soil dry weight
- Reference substance (positive control):
- yes
- Remarks:
- Cyanoguanidine, tested yearly.
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- other: LOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
- Details on results:
- - Effect concentrations exceeding solubility of substance in test medium: none reported
- Other: There was a decrease in pH values with increasing test material concentration beginning at 99.4 mg active acid/kg soil dry weight. See 'Any other information on results including tables' - Results with reference substance (positive control):
- at 50 mg reference item / kg soil dry weight, 75%, 100% and 93% inhibition of nitrate formation rate was observed after 0-7 days, 0-14 days and 0-29 days compared to the control.
- Reported statistics and error estimates:
- One Way Analysis of Variance (ANOVA) and Dunnett’s test was carried out for the determination of statistically significant differences compared to control replicates. A Normality test and an Equal Variance test was conducted first. P-values for both Normality and Equal Variance test are 0.05. As no inhibitions of nitrate-N content were observed, no ECx values could be calculated.
- Validity criteria fulfilled:
- yes
- Remarks:
- Variation between control replicates was < 15 % at days 0, 7, 14 and 28 for the determination of inorganic nitrogen (nitrate-N content).
- Conclusions:
- 28-day NOEC, EC10 and EC50 values of ≥1000, >1000 and >1000 mg active acid/kg soil dry weight have been determined for the effects of the test substance on the inhibition of nitrate formation of soil microbes, based on nominal concentrations.
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to Annex 3 of the CSR or IUCLID Section 13 for justification of read-across within the HEBMP category.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 28 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- other: LOEC
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- other: active acid
- Basis for effect:
- nitrate formation rate
Referenceopen allclose all
Table 1. Inhibition of Nitrate-N content in soil containing the test material, relative to the control
Test item concentration (mg active acid/kg soil dry weight) |
Inhibition (%) compared to control |
|||
Day 0 |
Day 7 |
Day 14 |
Day 28 |
|
3.16 |
-12 |
-1 |
0 |
2 |
9.83 |
-6 |
-4 |
-3 |
7 |
31.4 |
-10 |
-14 |
-17 |
-16 |
99.4 |
-11 |
-13 |
-20 |
-14 |
314 |
1 |
-39 |
-48 |
-58 |
1000 |
-5 |
-43 |
-51 |
-59 |
Table 2. Inhibition of Nitrate-N formation rates in soil containing the test material, relative to the control
Test item concentration (mg active acid/kg soil dry weight) |
Inhibition (%) compared to control |
||
Day 0 - 7 |
Day 0 - 14 |
Day 0 -28 |
|
3.16 |
4 |
4 |
5 |
9.83 |
-3 |
-2 |
10 |
31.4 |
-16 |
-18 |
-17 |
99.4 |
-15 |
-22 |
-15 |
314 |
-55 |
-59 |
-70 |
1000 |
-59 |
-62 |
-69 |
Table 3. Moisture and pH values of the soil after 0 and 28 days
Test concentration |
Soil dry weights (%) |
Moisture content as (%) of MWHC* |
pH values |
|||
(mg active acid/kg soil dry weight) |
day 0 |
day 28 |
day 0 |
day 28 |
day 0 |
day 28 |
Control |
86.4 |
86.5 |
44.2 |
43.7 |
6.45 |
6.36 |
3.16 |
86.2 |
86.3 |
45.0 |
44.6 |
6.46 |
6.34 |
9.83 |
86.1 |
86.2 |
45.1 |
44.9 |
6.39 |
6.34 |
31.4 |
86.1 |
86.3 |
45.2 |
44.3 |
6.36 |
6.28 |
99.4 |
86.1 |
86.3 |
45.1 |
44.5 |
6.40 |
6.22 |
314 |
86.1 |
86.5 |
45.1 |
43.9 |
6.32 |
6.02 |
1000 |
86.3 |
86.4 |
44.4 |
44.2 |
6.09 |
5.76 |
* MWHC - maximum water holding capacity
Results of the additional highest concentration (1000 mg active acid/kg dry weight) without the addition of the Lucerne-grass-green meal:
Table 4. Inhibition of Nitrate-N content
Nitrate-N Content |
||||
Test item concentration (mg active acid/kg soil dry weight) |
Inhibition (%) compared to control |
|||
d 0 |
d 7 |
d 14 |
d 28 |
|
Additional control |
-183 |
-23 |
-2 |
3 |
Table 5. Inhibition of Nitrate-N formation rate
Nitrate-N Formation Rate |
|||
Test item concentration (mg active acid/kg soil dry weight) |
Inhibition (%) compared to control |
||
d 0-7 |
d 0-14 |
d 0-28 |
|
Additional control |
42 |
40 |
41 |
Table 6. Moisture and pH values of the soil after 0 and 28 days
Test concentration |
Soil dry weights (%) |
Moisture content as (%) of MWHC |
pH values |
|||
(mg active acid/kg soil dry weight) |
day 0 |
day 28 |
day 0 |
day 28 |
day 0 |
day 28 |
Control |
86.4 |
86.5 |
44.2 |
43.7 |
6.45 |
6.36 |
Additional control |
86.7 |
86.7 |
43.1 |
42.9 |
5.70 |
5.64 |
Description of key information
28-day NOEC, EC10 and EC50 values of ≥1000, >1000 and >1000 mg active acid/kg soil dry weight have been determined for the effects of HEBMP-xNa on the nitrate formation rate of soil microbes.
Key value for chemical safety assessment
Additional information
- HEBMP is present as HEBMP-H or one of its ionised forms. The degree of ionisation depends upon the pH of the media and not whether HEBMP-xNa salt, HEBMP-H (acid form), or another salt was used for dosing.
- Disassociated sodium cations. The amount of sodium present depends on which salt was added.
- It should also be noted that divalent and trivalent cations would preferentially replace the potassium ions. These would include calcium (Ca2+), magnesium (Mg2+) and iron (Fe3+).
28-day NOEC, EC10 and EC50 values of ≥1000, >1000 and >1000 mg active acid/kg soil dry weight respectively have been determined for the effects of HEBMP-xNa on the nitrate formation rate of soil microbes, expressed in terms of nominal concentrations (Noack, 2021). The study was conducted according to OECD TG 216, in compliance with GLP and met the validity criteria, therefore it was assigned a reliability value of 1.
The acid and sodium salts in the HEBMP category are freely soluble in water. The HEBMP anion can be considered fully dissociated from its sodium cation when in dilute solution. Under any given conditions, the degree of ionisation of the HEBMP species is determined by the pH of the solution. At a specific pH, the degree of ionisation is the same regardless of whether the starting material was HEBMP-H, HEBMP-xNa, or another salt of HEBMP.
Therefore, when a salt of HEBMP is introduced into test media or the environment, the following is present (separately):
The terrestrial environment is exposed to HEBMP predominantly via application of WWTP sludge, and as such this phenomenon also directly affects soil in the receiving environment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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