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EC number: 911-811-2 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 May - 16 June 2021
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: all test concentrations
- Sampling method: At the start of the exposure-renewal intervals (0 hours), samples of the fresh media were taken and analyzed after preparation of all concentrations and the control. At the end of the exposure-renewal intervals (48 or 72 hours), samples of the old media were taken from the test vessels.
- Sample storage conditions before analysis: All samples were stored at room temperature until start of analysis, if necessary. Prepared samples were stored in the autosampler at room temperature until analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method:
A stock solution of 118 mg active acid/L was prepared with the culture medium without EDTA for each exposure interval (test item was weighed and added to dilution water, then agitated until the solution was clear). Test solutions were prepared by dilution of the stock solution with dilution water without EDTA.
- Controls:
dilution water without EDTA
- Evidence of undissolved material (e.g. precipitate, surface film, etc.):
no evidence of undissolved material reported - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name:
water flea
- Strain/clone: Not reported
- Age at study initiation (mean and range, SD):
less than 24 hours old
- Weight at study initiation (mean and range, SD): not reported
- Length at study initiation (length definition, mean, range and SD):
not reported
- Method of breeding:
daphnids were held in glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 +/- 2 °C, in an incubator, 16 hours illumination; light intensity of max. 1500 lx.
- Source:
Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
- Age of parental stock (mean and range, SD):
no first brood progeny was used for the test, no further details reported.
- Feeding during test
- Food type:
Pseudokirchneriella subcapitata (0.353 – 0.551 mL) and Desmodesmus subspicatus (0.346 – 0.627 mL) suspension
- Amount:
max. 0.2 mg C per Daphnia and day.
- Frequency: daily
ACCLIMATION
- Acclimation period:
at least 2 hours
- Acclimation conditions (same as test or not):
same as the test (dilution media without EDTA)
- Type and amount of food:
not reported
- Feeding frequency: not reported
- Health during acclimation (any mortality observed):
not reported - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Hardness:
- 237 - 281 mg/L as CaCO3
- Test temperature:
- 19.1 - 20.9 degrees C
- pH:
- 6.41 - 8.08
- Dissolved oxygen:
- 5.27 - 9.57 mg/l
- Salinity:
- not reported
- Conductivity:
- not reported
- Nominal and measured concentrations:
- Nominal: control, 3.93, 9.04, 20.8, 47.9 and 110 mg active acid/L
TWA mean measured: control, 3.91, 8.21, 19.1, 45.6, 106 mg active acid/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: glass beakers (5 (ID) x 8 (H) cm)
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass, 100mL capacity, 50mL test volume
- Aeration: test vessels were not aerated during the test
- Renewal rate of test solution (frequency/flow rate): medium renewal of the test solutions was 3 times per week
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Biomass loading rate: not reported
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 culture medium
- Total organic carbon: not reported
- Particulate matter: not reported
- Metals: not reported
- Pesticides: not reported
- Chlorine: not reported
- Alkalinity: not reported
- Ca/mg ratio: not reported
- Conductivity: not reported
- Culture medium different from test medium: No
- Intervals of water quality measurement: The water quality parameters (i.e. pH-values, dissolved oxygen concentration, total hardness and water temperature) were measured once within 7 days, in the fresh media at the start of an exposure-renewal interval (0 hours) and in the old media at the end of an exposure-renewal interval (48 or 72 hours), in one replicate of the control and the highest concentration of the test item with a living parent animal.
The water quality parameters in fresh media were measured in an additional replicate without daphnids of the highest concentration of the test item and the control. At the end of the exposure-renewal interval, the water quality parameters of the old media were measured in a test vessel of the highest concentration of the test item and the control, which contains daphnids and food algae.
OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: 16/8 hours light/dark cycle
- Light intensity: max. 1500 lx
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality and immobilisation of daphnids were observed once a day at least. The first appearance of juveniles was checked daily, the number of neonates were counted once a day from the first appearance of neonates. The number of aborted eggs or dead offspring and the dates of observation were recorded.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.3 - Reference substance (positive control):
- no
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- mortality
- Details on results:
- - Behavioural abnormalities:
behaviour of daphnids was observed each day of observation, but no abnormalities were reported
- Observations on body length and weight:
see Table 4
- Other biological observations:
no male daphnids or ephippia (winter eggs) were observed in the control or test groups during the test.
- Mortality of control:
0% mortality in the control at 7, 14 and 21 days
- Other adverse effects control: no adverse effects were observed in the control group
- Immobilisation of control: 0% immobilisation in the control group
- Abnormal responses:
some stillborn juveniles and aborted eggs were observed, see Table 3
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none reported
- Effect concentrations exceeding solubility of substance in test medium: not reported - Reported statistics and error estimates:
- NOEC and LOEC values were determined using Dunnett's multiple t-test for reproduction and Fisher's exact Binomial test with Bonferroni correction for adult mortality. Data were analysed for normality and variance and homogeneity using Shapiro-Wilk's and Levene's tests, respectively.
ECx values were derived empirically from the observed data. - Validity criteria fulfilled:
- yes
- Remarks:
- Mortality in the control group was 0% and the mean number of living offspring produced per introduced parent animal at the end of the test was 98 in the control group.
- Conclusions:
- 21-day NOEC, EC10 and EC50 values of ≥106, >106 and >106 mg active acid/L have been determined for the effects of the test substance on the reproduction and mortality of Daphnia magna, based on time weighted average measured concentrations.
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to Annex 3 of the CSR or IUCLID Section 13 for justification of read-across within the HEBMP category.
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 106 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- other: active acid
- Basis for effect:
- mortality
Referenceopen allclose all
Table 1. LC-MS/MS analysis of the test item and equivalent active acid concentrations
Nominal concentration (mg test item/L) |
Day 0 fresh medium 0 hours (mg test item/L) |
Day 2 old medium 48 hours (mg test item/L) |
Day 9 fresh medium 0 hours (mg test item/L) |
Day 12 old medium 72 hours (mg test item/L) |
Day 14 fresh medium 0 hours (mg test item/L) |
Day 16 old medium 48 hours (mg test item/L) |
TWA mean measured concentration (mg test item/L) |
TWA mean measured concentration (mg active acid/L) |
Control |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
<LOQ |
Control |
Control |
10.0 |
9.92 |
11.0 |
8.20 |
11.0 |
10.2 |
9.65 |
9.94 |
3.91 |
23.0 |
21.4 |
22.6 |
17.7 |
21.2 |
20.2 |
24.2 |
20.9 |
8.21 |
52.9 |
51.4 |
43.1 |
44.6 |
49.6 |
52.1 |
54.2 |
48.7 |
19.1 |
122 |
117 |
95.2 |
114 |
121 |
135 |
113 |
116 |
45.6 |
280 |
288 |
260 |
263 |
258 |
311 |
238 |
269 |
106 |
Table 2. Mean reproduction and mortality ofDaphnia magnaadults after 21 days
TWA mean measured concentration (mg active acid/L) |
Mean number of offspring per survived parental daphnid |
Mortality |
||||
Mean |
SD |
CV |
Day 7 |
Day 14 |
Day 21 |
|
Control |
98.2 |
6.05 |
6.2 |
0 |
0 |
0 |
3.91 |
96.0 |
10.6 |
11.1 |
0 |
0 |
0 |
8.21 |
92.0 |
9.64 |
10.5 |
0 |
0 |
10 |
19.1 |
94.3 |
15.1 |
16.1 |
0 |
0 |
0 |
45.6 |
103 |
8.60 |
8.3 |
0 |
0 |
0 |
106 |
98.3 |
15.0 |
15.3 |
0 |
0 |
0 |
Table 3. Biological observations of reproduction inDaphnia magnaafter 21 days
TWA mean measured concentration (mg active acid/L) |
Mean intrinsic rate of natural increase |
Mean day of first appearance of living juveniles |
Number of stillborn juveniles |
Number of aborted eggs |
||
Mean |
SD |
CV |
||||
Control |
0.344 |
0.0252 |
7.3 |
8.3 |
44 |
0 |
3.91 |
0.343 |
0.0195 |
5.7 |
8.2 |
3 |
1 |
8.21 |
0.331 |
0.0190 |
5.7 |
8.7 |
3 |
0 |
19.1 |
0.331 |
0.0244 |
7.4 |
9.0 |
1 |
1 |
45.6 |
0.352 |
0.0193 |
5.5 |
8.0 |
1 |
0 |
106 |
0.333 |
0.0243 |
7.3 |
8.6 |
0 |
0 |
Table 4. Mean body length and weight of parent daphnids after 21 days
TWA mean measured concentration (mg active acid/L) |
Mean length of survived parent daphnids (mm) |
Mean dry weight of parent daphnids (mg) |
Control |
4.25 |
0.70 |
3.91 |
4.20 |
0.59 |
8.21 |
4.17 |
0.62 |
19.1 |
4.15 |
0.82 |
45.6 |
4.35 |
0.81 |
106 |
4.25 |
0.79 |
Description of key information
21-day NOEC and EC10 values of ≥106 mg active acid/L and >106 mg active acid/L have been determined for the effects of HEBMP-xNa on the reproduction and mortality of Daphnia magna.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC10
- Effect concentration:
- > 106 mg/L
Fresh water invertebrates
- Dose descriptor:
- NOEC
- Effect concentration:
- >= 106 mg/L
Additional information
- HEBMP is present as HEBMP-H or one of its ionised forms. The degree of ionisation depends upon the pH of the media and not whether HEBMP-xNa salt, HEBMP-H (acid form), or another salt was used for dosing.
- Disassociated sodium cations. The amount of sodium present depends on which salt was added.
- It should also be noted that divalent and trivalent cations would preferentially replace the potassium ions. These would include calcium (Ca2+), magnesium (Mg2+) and iron (Fe3+).
21-day NOEC, EC10 and EC50 values of ≥106, >106 and >106 mg active acid/L respectively have been determined for the effects of HEBMP-xNa on adult reproduction and mortality of Daphnia magna (Noack, 2021). The test concentrations were analysed by LC-MS/MS analysis and the results are expressed in terms of time weighted average (TWA) measured concentrations. The study was conducted according to OECD TG 211, in compliance with GLP and met the validity criteria, therefore it was assigned a reliability value of 1.
The acid and sodium salts in the HEBMP category are freely soluble in water. The HEBMP anion can be considered fully dissociated from its sodium cation when in dilute solution. Under any given conditions, the degree of ionisation of the HEBMP species is determined by the pH of the solution. At a specific pH, the degree of ionisation is the same regardless of whether the starting material was HEBMP-H, HEBMP-xNa, or another salt of HEBMP.
Therefore, when a salt of HEBMP is introduced into test media or the environment, the following is present (separately):
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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