1-methylpiperidine

Specific investigations: other studies

Administrative data

Endpoint:

specific investigations: other studies

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards, well documented and acceptable for assessment.

Data source

Reference

Reference Type:

publication

Title:

Effect of tobacco smoke compounds on the plasma membrane of cultured human lung fibroblasts.

Author:

Thelestam, M., Curvall, M., Enzell, C.R.

Year:

1980

Bibliographic source:

Toxicology, 15, 203-217

Materials and methods

Principles of method if other than guideline:

Cultured human fibroblasts were incubated with N-methylpiperidine, a solvent alone and a positive control reference. Liberation of uridine nucleotides through the cell membrane was the basis for assessment of the membrane damaging capability of the test substance, in comparison with the negative and positive reference.

GLP compliance:

not specified

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

N-Methyl Piperidine

IUPAC Name:

N-Methyl Piperidine

Administration / exposure

Details on exposure:

Cultured human fibroblasts (line MRC-5, 10xE5 cells/cm², labelled with ³H uridine) were incubated with N-methylpiperidine (2480 mg/L, 30 min at 37°C in TRIS-buffered 0.15 mM saline) and solvent alone. Additionally a positive control reference (Scraping with a rubber policeman) was applied. Liberation of uridine nucleotides through the cell membrane was the basis for assessment of the membrane damaging capability of the test substance, in comparison with the negative and positive reference.

After incubation the solution containing leaked radioactive marker was removed and centrifuged (1000 g, 10 min, 4°C) and radioactivity in 0.1 mL aliquots of the supernatant was measured by liquid scintillation. A maximal release of the radioactivity was obtained by treating control cells for 30 min with 0.06 M sodium borate buffer (pH 7.8) and scraping with a rubber policeman. This treatment ruptured the cell membranes leaving the nuclei intact. The following expression was used to calculate the relative leakage of the radioactive marker:
(experimental release - spontaneous release)/ (maximal release - spontaneous release) x 100.
The spontaneous release of the nucleotide marker during incubation for 30 min at 37°C was 3-7 per cent of the maximal release.

Duration of treatment / exposure:

30 min

Doses / concentrations

Remarks:

Doses / Concentrations:
2480 mg/L
Basis:
no data

Examinations

Examinations:

Determination of the leakage of the radioactive marker.

Results and discussion

Details on results:

N-Methyl Piperidine was classified as a substance with strong membrane damaging potential, based on the strong liberation of uridine nucleotides of 80%.