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EC number: 224-314-4 | CAS number: 4303-67-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 - 8 Apr 2002
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, non-GLP
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- no
Test material
- Reference substance name:
- laurylimidazole
- IUPAC Name:
- laurylimidazole
- Details on test material:
- - Name of test material (as cited in study report): Laurylimidazol
- Physical state: liquid
- Analytical purity: 99%
- Storage condition of test material: at room temperature (ca. 20 °C) in darkness away from direct sunlight
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Laurylimidazole (100 mg/L) was weighed into test water and sonicated 15 minutes, followed by 48 hours of intensive stirring. The solution was then filtered under reduced pressure through a 0.45 µm membrane filter (Schleicher & Schuell NC45).
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): No vehicle
- Evidence of undissolved material (e.g. precipitate, surface film, etc): none, test medium was a clear solution throughout experiment
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Strain: D. magna Straus
- Source: Clone propagated from original, provided 1992 by University of Sheffield.
- Age at study initiation (mean and range, SD): 6-24 hours, not first brood progeny
- Method of breeding: Organisms bred under same light, temperature, and water quality conditions as in the test
- Feeding during test: No
ACCLIMATION
- Acclimation period: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 250 mg/L as CaCO3
- Test temperature:
- 21 °C
- pH:
- 7.7 - 7.8
- Dissolved oxygen:
- 8.0 - 8.5 mg/L
- Nominal and measured concentrations:
- 100 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Glass beakers (100 mL) with glass covers, filled with 50 mL test medium.
- Aeration: none
- No. of organisms per vessel: Ten
- No. of vessels per concentration (replicates): Two
- No. of vessels per control (replicates): Ten
- No. of vessels per vehicle control (replicates): Two
- Biomass loading rate: < 1 Daphnia/ 2mL
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water
294.0 mg/L CaCl2 x 2∙H2O
123.0 mg/L MgSO4 x 7∙H2O
65.0 mg/L NaHCO3
5.8 mg/L KCl
- Alkalinity: 0.8 mmol/l
- Ca/mg ratio: 4:1
- Aeration: Medium was aerated until oxygen saturation was reached prior to study, no further aeration.
- Culture medium different from test medium: Same medium
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark, with 30 minute transition periods.
- Light intensity: 200-1200 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobiliization at 24 and 48 hours
TEST CONCENTRATIONS
- Spacing factor for test concentrations: None
- Justification for using less concentrations than requested by guideline: Limit test
- Range finding study: no
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: water soluble fraction
- Basis for effect:
- mobility
- Remarks on result:
- other: no immobilization
- Details on results:
- - Behavioural abnormalities: No immobilized test organisms or other signs of intoxication were observed during the test.
- Mobility of control: No immobilized controls.
- Effect concentrations exceeding solubility of substance in test medium: yes
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-h EC50 (immobilization) of laurylimidazole to D. magna is > 100 mg/L as water-soluble fraction (OECD 202)
- Executive summary:
Toxicity of laurylimidazole to D. magna was assessed in a limit test conducted according to OECD 202. The test substance was not fully soluble at the limit of 100 mg/L, so a water-soluble fraction was made. Test substance (100 mg/L) was sonicated in reconstituted test water for 15 minutes, stirred for 48 hours, and filtered to 0.45 µm under reduced pressure. No immobilization or sublethal effects were observed in controls or test chambers. Test substance concentrations were not analytically determined. The 48-hour EC50 of laurylimidazole is >100 mg/L.
The test was conducted according to an internationally accepted guideline but was not GLP compliant. Test substance concentrations were not analytically confirmed. This test is considered reliable with restrictions. It is suitable for Risk Assessment, Classification & Labeling, and PBT Analysis.
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