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EC number: 232-260-8 | CAS number: 7803-51-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Protocols for the care, treatment and killing of the mice were approved by the Animal Care Committees of the Health Effects Research Laboratory of the US EPA and the NIEHS and meet all guidelines set by the National Institutes of Health.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- publication
- Title:
- Cytogenetic and germ cell effects of phosphine inhalation by rodents: II. Subacute exposures to rats and mice.
- Author:
- Kligerman A.D., Bishop J.B., Erexson G.L., Price H.C., O'Connor R.W. and Morgan D.L.
- Year:
- 1 994
- Bibliographic source:
- Environ Mol Mutagen 24(4):301-306
Materials and methods
- Principles of method if other than guideline:
- Rodents are exposed to target concentrations of phosphine by inhalation for an appropriate period and are sacrificed at appropriate times after treatment. Specific cells are collected (blood lymphocytes) and treated to study specific endpoints chromosome aberrations
- GLP compliance:
- not specified
- Type of assay:
- chromosome aberration assay
Test material
- Reference substance name:
- Phosphine
- EC Number:
- 232-260-8
- EC Name:
- Phosphine
- Cas Number:
- 7803-51-2
- Molecular formula:
- H3P
- IUPAC Name:
- phosphane
- Details on test material:
- gas was procured in cylinders containing 2,500 ppm PH3 in nitrogen (AGA specialty Gas, Inc, Maumee, OH).
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Fischer 344
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- After exposition, male rat (approximately 8 weeks of age) were returned to the holding cages with food and tap water ad libitum. Animals wera maintened on a 12 hours light/dark cycle with controlled temperature and humidity.
Administration / exposure
- Route of administration:
- inhalation: gas
- Vehicle:
- The desired exposure concentration were obtained by introducing metered quantites of PH3 directly into the process air stream just prior to a set of static mixing element.
- Details on exposure:
- Male rats were placed in the inhalation chambers without food or water and exposed to target concentration.
- Duration of treatment / exposure:
- 9 days over 11 day period (5 days exposed, 2 days off, 4 days exposed)
- Frequency of treatment:
- 6 hours per day.
- Post exposure period:
- 18 to 20 hours.
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
1.25 ppm
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
2.5 ppm
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
5 ppm
Basis:
nominal conc.
- No. of animals per sex per dose:
- 5 animals per dose
- Control animals:
- yes
- Positive control(s):
- No data
Examinations
- Tissues and cell types examined:
- blood lymphocytes
- Details of tissue and slide preparation:
- Blood was removed from the rats by cardiac puncture.
2 ml cultures were established containing 5*10E5 mononuclear leukocytes, RPMI-1640, 10% heat-inactivated fetal bovine serum, an additionnal 1% L-Glutamine, 1% penicillin-streptomycin and 10 µg/ml concanavalin A.BrdUrd (5µM) was added at 21 hours for analyses of sisterchromatid exchange and cell cycle kinetics and the culture was harvested by cytocentrifugation at 54 hours of culture following a 3 hours demecocline treatment.
Cells were treated with a hypotonic 0.075M KCl solution and fixed in 3:1 methanol:acetic acid. Slides were made, mounted ans stained according to Erexson and Kligerman (1987). - Evaluation criteria:
- For each animal, 100 first division metaphases were scored for chromosome aberrations. The replicative index was calculated from 100 consecutive metaphases.
- Statistics:
- For chromosome aberration and percentage polychromatic erythrocytes date, the trend test was performed.
The level of significance chosen was 0.05.
Results and discussion
Test results
- Sex:
- male
- Genotoxicity:
- negative
- Remarks:
- Peripheral blood lymphocyte
- Toxicity:
- no effects
- Vehicle controls validity:
- valid
- Negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Additional information on results:
- PH3 inhalation caused no statistically significant increases in Chromsome aberrations in peripheral blood lymphocytes
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information): negative Peripheral blood lymphocyte
No increase in cytogenetic end points (chromosome aberration) was observed over controls in cultured lymphocytes.
The concentrations of PH3 up to 5 ppm (7.1 mg/m3) are not genotoxic to rodents when administrated by inhalation for 9 days during an 11 day period. - Executive summary:
Male F344/N rats were exposed to 0 ; 1.25 ; 2.5 or 5 ppm of phosphine to 6 hours per day for 9 days over an 11 days period. Approximately 20 hours after the termination of exposure, blood was removed from the rats and the lymphocytes cultured for analyses chromosome aberration. No significant increase in chromosome aberration was observed over controls in cultured lymphocytes.
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