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EC number: 224-709-1 | CAS number: 4457-71-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997-02-21 to 1997-03-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: The study was well conducted by a GLP-compliant laboratory and according to an OECD guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 202 "Acute immobilization test and reproduction test of water flea"
- Version / remarks:
- 1984
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-methyl-1,5-pentanediol
- IUPAC Name:
- 3-methyl-1,5-pentanediol
- Reference substance name:
- 3-methylpentane-1,5-diol
- EC Number:
- 224-709-1
- EC Name:
- 3-methylpentane-1,5-diol
- Cas Number:
- 4457-71-0
- Molecular formula:
- C6H14O2
- IUPAC Name:
- 3-methylpentane-1,5-diol
Constituent 1
Constituent 2
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 3-methyl-1,5-pentanediol
- Molecular formula: C6H14O2
- Molecular weight: 118.17
- Physical state: Colorless clear liquid
- Analytical purity: 99.8%
- Impurities (identity and concentrations): Water content 0.04%; Acid (as CH3COOH) less than 0.02%
- Lot/batch No.: WDK5104
- Storage condition of test material: stored in a refrigerator in Kurume Laboratory; stable under storage conditions, tested with IR
- Other: Supplier: Wako Pure Chemicals Industries Ltd.
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- For each test medium, aliquot of samples was collected from the middle part of each of the test vessels, mixed, and analysed, directly or after dilution, by GC, 6 times during the whole exposure period (3 times just before and after water change), (for the samples before water changes, feed was removed by centrifuge, 10 min at 3000 rpm). For this analysis a standard solution (conc. 50 mg/L) was also measured and used to quantify the amount by peak area ratio.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was dissolved in de-chlorinated tap water to produce a stock solution of 1,000 mg/L.
The stock solution was further diluted with tap water to gain 3 concentration levels as used in the test (10, 50, 25 mg/L).
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: The original was purchased from U.S. EPA Environmental Research Laboratory, Duluth and subcultured in the Kurume laboratory
- Age of parental stock (mean and range, SD): 2 ~ 4 weeks (parent of immatures from subculture)
- Age at study initiation (mean and range, SD): immature aged 24 hours or less
- Feeding during test: Yes
- Food type: Unicellar algae (Chlorella Vulgaris); centrifuged by replacing with dilution water
- Amount:0.1 ~ 0.2 mgC(organic carbon content)/water flea (The amount was changed within this range depending on the growth stage of water feleas)
- Frequency: daily
ACCLIMATION
- Acclimation conditions (same as test or not): Same
- Type and amount of food: see above
- Feeding frequency: see above
-Culture density: 10 water fleas/800 mL culture water
- Health during acclimation (any mortality observed): batch with high death rate or sleeping eggs or males were not used for the test.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 19.9 - 20.5 °C
- pH:
- 7.2 - 7.7
- Dissolved oxygen:
- 6.9 - 8.8 mg/L
- Nominal and measured concentrations:
- 100, 50, 25 mg/L nominal
measured: 97.2 ~ 103% of nominal value at the time of preperation of the test solution
measured: 91.8 - 106% of nominal value after 2 ~ 3 days (before water change) - Details on test conditions:
- TEST SYSTEM
-Test vessel: 1000 mL glass vessel (diameter 12.0 cm, height 12.5 cm)
- Renewal rate of test solution (frequency/flow rate): 3 times a week; test organisms were placed into fresh test solutions three times a week
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Amount of test solution: 800 mL/vessel
- Biomass loading rate: 40 water fleas/L replicate
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated water; tap water was dechlorinated and treated by activated carbon. Before using the dilution water, aeration was fully carried out.
Dilution water chemistry:
- pH: 7.7
- Total hardness (converted to CaCO3): 55.6 mg/L
- COD: 0.2 mg/L
- Metals: not detected: total mercury, copper, cadmium, zinc, lead, aluminium, nickel, total chromium, manganese, tin. 0.05 mg/L Iron
- Pesticides: not detected
- Free chlorine: not detected
- Alkalinity: 37.5 mg/L
- Conductivity: 171 µs/cm
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Photoperiod: 16 - 8 hour light-dark photocycle
- Light intensity: room light
- Test temperature: 20 +- 1°C (adjusted by Thermostat)
- Intervals of water quality measurement: Water temperature, dissolved oxygen concentration, and pH for all test medium (1 test vessel each) were measured in total 10 times (5 times before and after water change) during exposure period.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Parent water fleas: Number of live parents, number of immobilized, size, and appearance were measured everyday, by comparing against the control. Dead parents were removed after counting.
- Produced juveniles: Number of dead and live juveniles, and generation of sleeping eggs were measured before water changes. The juveniles were removed after counting. First brood production day was recorded.
RANGE-FINDING STUDY
From the results of acute immobilization test [OECD 202 Part I entered in the endpoint robust study summary 6.1.3 Kurume (1997); 91742; 48h EiC50 >1000 mg/L] and preliminary test results, the test concentration were set at 100, 50, 25 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate, reagent ultra pure grade; Wako Pure Chemicals Industries Ltd.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- reproduction
- Remarks:
- on cumulative number of juveniles produced after 21 day, per parent
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- reproduction
- Remarks:
- on cumulative number of juveniles produced after 21 day, per parent
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- reproduction
- Remarks:
- inhibition
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality
- Remarks:
- for parent water fleas
- Details on results:
- - Mortality of parent animals: no
- Body length and weight of parent animals: no difference of size and conditions of Daphnia was observed between the control and the test medium
- Time to first brood release or time to hatch: day 7 for control and all test concentrations
- Effect concentrations exceeding solubility of substance in test medium: no - Results with reference substance (positive control):
- confirmation of sensitivity: EiC50(48 hr) by reference substance potassium dichromate, reagent ultra pure grade was 0.283 mg/L. It has not been described whether the data are historical reference data of the Kurume laboratory or the U.S. EPA Environmental Research Laboratory.
Any other information on results incl. tables
Nominal concentrations: 25, 50 and 100 mg/L
Measured concentrations:
Nominal concentration Concentration of
(mg/L) 3-metyl-1,5-pentanediol (mg/L)
Range Mean
Control n. d. n. d.
25.0 23.2 - 25.7 24.4
50.0 45.9 - 51.7 49.6
100 96.3 - 106 102
n. d. :<5.00 mg/L
Biological observations
Cumulative numbers of dead parental Daphnia:
Nominal concentration Cumulative number of
(mg/L) dead parental Daphnia Exposure time (day)
7 14 21
0 0(0) 0(0) 0(0)
25.0 0(0) 0(0) 2(5.0%)
50.0 0(0) 0(0) 0(0)
100 0(0) 0(0) 1(2.5%)
There is no statisitcally significant difference from control for
mortality
Time of the first production of young: 7 days for control and all
testconcentrations
Mean cumulative numbers of young produced per adult:
Nominal concentration Mean cumulative numbers of young S.D
(mg/L) produced per adult at 21 days
0 86.1
13.9
25.0 102
11.9
50.0 109
4.4
100 98.7
9.8
There is no statisitcally significant difference from control for number of juveniles produced
Was control response satisfactory: Yes
Applicant's summary and conclusion
- Conclusions:
- NOEC reproduction inhibition > = 100 mg/L
- Executive summary:
This study was performed in compliance with OECD Guideline No. 202 (1984) Part II for reproduction with water fleas to test the substance MPD with the test concentration 100, 50 and 25 mg/L and control. Under the reported test conditions a LC50 of parent fleas in 21 days was > 100 mg/L. The ErC50 in 21 days was >100 mg/L and the LOECr in 21 days was >100 mg/L.
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