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Diss Factsheets
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EC number: 201-321-0 | CAS number: 81-07-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- Preliminary assessment of the skin sensitizing activity of selected rodent carcinogens using the local lymph node assay
- Author:
- E.Vicky Warbrick a, Rebecca J. Dearman a, John Ashby a, Peter Schmezer b, Ian Kimber a
- Year:
- 2 001
- Bibliographic source:
- Toxicology 163 (2001) 63–69
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other:
- Principles of method if other than guideline:
- Groups of mice (n=4) were exposed topically on the dorsum of both ears to 25 l of various concentrations of the test chemicals, or to the same volume of the relevant vehicle alone, daily for 3 consecutive days. Five days following initiation of exposure all mice were injected via the tail vein with 250 l of phosphate buffered saline (PBS) containing 20 Ci of [3H] methyl thymidine. Five hours later the mice were sacrificed and the draining auricular lymph nodes excised and pooled for each experimental group. Single cell suspensions of LNC were prepared by mechanical disaggregation through 200-mesh stainless steel gauze. Pooled LNC were washed twice with PBS and precipitated in 5% trichloroacetic acid (TCA) at 4°C overnight. Pellets were then resuspended in 1 ml of TCA and transferred to 10 ml of scintillation fluid (Optiphase ‘Hisafe 3’, Wallac, Turku, Finland). The incorporation of 3H-TdR was measured by -scintillation counting as disintegrations per minute (dpm) per node for each experimental group. In each case a stimulation index (SI) relative to the concurrent vehicle-treated control was
derived; an SI of 3 or greater being indicative of a chemical possessing the potential to cause contact sensitization - GLP compliance:
- not specified
- Type of study:
- mouse local lymph node assay (LLNA)
Test material
- Reference substance name:
- 1,2-benzisothiazol-3(2H)-one 1,1-dioxide
- EC Number:
- 201-321-0
- EC Name:
- 1,2-benzisothiazol-3(2H)-one 1,1-dioxide
- Cas Number:
- 81-07-2
- Molecular formula:
- C7H5NO3S
- IUPAC Name:
- 1,2-benzisothiazol-3(2H)-one 1,1-dioxide
- Details on test material:
- - Name of test material (as cited in study report): 1,2-benzisothiazol-3(2H)-one 1,1-dioxide
- Substance type: Organic
- Physical state: solid
Constituent 1
In vivo test system
Test animals
- Species:
- mouse
- Strain:
- CBA
- Sex:
- female
- Details on test animals and environmental conditions:
- Young adult (8–12 weeks old) CBA/Ca strain female mice (Harlan Seralab, Oxon, UK) were used throughout these studies. Animals were housed four per cage on flushing metal racks. Food (SDS PCD pelleted diet; Special Diets Services, Witham, Essex, UK) and water were available ad libitum.
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal and epicutaneous
- Vehicle:
- DMSO
- Concentration / amount:
- 0, 25, 50 and 75 %
Challenge
- Concentration / amount:
- 0, 25, 50 and 75 %
Study design: in vivo (LLNA)
- Vehicle:
- dimethyl sulphoxide
- Concentration:
- 25 µl
- No. of animals per dose:
- total: 16
0 %: 4 female
25 %: 4 female
50 %: 4 female
70 %: 4 female
Results and discussion
In vivo (LLNA)
Results
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: 536 dpm/node to 803 dpm/node
Any other information on results incl. tables
The maximal stimulation indices for saccharin was 1.50, at a test concentration of 50%. Saccharin, which failed to stimulate a positive response at any concentration tested, EC3 values of 50 and > 75 %, respectively, were assigned, according to the maximum concentrations tested in each case.
Applicant's summary and conclusion
- Interpretation of results:
- not sensitising
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- The maximal stimulation indices for saccharin was 1.50, at a test concentration of 50%. Saccharin, which failed to stimulate a positive response at any concentration tested, EC3 values of 50 and > 75 %, respectively, were assigned, according to the maximum concentrations tested in each case.
- Executive summary:
The maximal stimulation indices for saccharin was 1.50, at a test concentration of 50%. Saccharin, which failed to stimulate a positive response at any concentration tested, EC3 values of 50 and > 75 %, respectively, were assigned, according to the maximum concentrations tested in each case.
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