Registration Dossier
Registration Dossier
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EC number: 274-986-8 | CAS number: 70892-34-1
Toxicity to aquatic plants other than algae
Administrative data
Link to relevant study record(s)
Description of key information
The test item C.I. Leuco Sulphur Brown 37 had no toxic effects on Lemna gibba during the 7-day test period up to its solubility limit in the test water under the present test conditions.
Key value for chemical safety assessment
- EC10 or NOEC for freshwater plants:
- 100 mg/L
Additional information
The influence of the test item C.I. Leuco Sulphur Brown 37 on the growth of the freshwater aquatic plant Lemna gibba (duckweed) was investigated in a 7‑day semi‑static test, based on the OECD Guideline No. 221 “Lemnasp. Growth Inhibition Test” (2006) and the Commission Regulation (EC) No. 761/2009, C.26.
Due to the low water solubility of the test item, a dispersion of the test item with the loading rate of 100 mg/L was ultrasonic treated for 15 minutes and continuously stirred at room temperature in the dark over 96 hours. Then, the dispersion was filtered. The undiluted filtrate with the loading rate of 100 mg/L and dilutions 1:2, 1:4, 1:8 and 1:16 of the undiluted filtrate with the loading rate of 100 mg/L were used as test media. This corresponds to theoretical concentrations of 100, 50, 25, 12.5 and 6.3 mg/L. Additionally, a control was tested in parallel.
The preparation of the test media was based on the OECD Guidance Document on Aquatic Toxicity Testing of Difficult Substances and Mixtures (2000).
No analytical work was performed on request of the Sponsor due to the specific properties of the test item (low solubility in water).
In conclusion, the test item C.I. Leuco Sulphur Brown 37 had no toxic effects on Lemna gibba during the 7-day test periodup to its solubility limit in the test water under the present test conditions.
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