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EC number: 240-367-6 | CAS number: 16260-09-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in water and sediment: simulation tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: sediment simulation testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 308 (Aerobic and Anaerobic Transformation in Aquatic Sediment Systems)
- Version / remarks:
- 2002
- GLP compliance:
- yes (incl. QA statement)
- Radiolabelling:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- natural water / sediment: freshwater
- Details on source and properties of surface water:
- - Location : Calwich Abbey Lake UK, Emperor Lake UK
- Storage conditions: 5 ± 3°C
- pH at time of collection: 7.79 in Calwich Abbey Lake UK, not given for Emperor Lake UK
- Oxygen concentration (% saturation) initial/final: not given
- Water filtered: yes
- Type and size of filter used, if any: Prior to use the sediment was passed through a 2 mm sieve and the water was passed through a 0.2 mm sieve. - Details on source and properties of sediment:
- - Location : Calwich Abbey Lake UK, Emperor Lake UK
- Storage conditions: 5 ± 3°C
- Textural classification (i.e. %sand/silt/clay): Silt Loam for Calwich Abbey Lake & Sandy clay loam for Emperor Lake
- Organic carbon (%): 4.4 and 2.8 for CAL and EL respectively
- CEC (meq/100 g): 12.1 and 9.7 for CAL and EL respectively
- Bulk density (g/cm³): not determined
- Sediment samples sieved: yes - Duration of test (contact time):
- 105 d
- Initial conc.:
- 10 µg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- radiochem. meas.
- Details on study design:
- TEST CONDITIONS
- Composition of medium:
Calwich Abbey Lake Emperor Lake
Total organic carbon (mg/L) 5.43 8.46
Total nitrogen (mg/L) 3.33 1.01
Total phosphorus (mg/L) 0.02 0.02
Total suspended solids (mg/L) 8.5 46.0
Hardness as CaCO3 (mg/L) 260 46.4
- Test temperature: 12 °C
- pH: pH in water 7.79 (CAL); not determined (EL)
- pH adjusted: no
- CEC (meq/100 g): 12.1 (meq/100 g) (CAL) and 9.7 (meq/100 g) (EL)
- Aeration of dilution water: yes
- Suspended solids concentration: Total suspended solids (mg/L) 10 for CAL & 4 for EL
- Continuous darkness: yes
- Any indication of the test material adsorbing to the walls of the test apparatus: no
- Test performed in closed vessels due to significant volatility of test substance: yes
- Test performed in open system: no
- Details of trap for CO2 and volatile organics if used: 4. Vessel containing 1 M aqueous potassium hydroxide solution with phenolphthalein indicator (to trap 14CO2)
SAMPLING
- Sampling frequency: Duplicate samples of each aquatic sediment were taken for analysis immediately after test item application and after 1, 7, 14, 29, 60 and 105 days of incubation.
- Sampling method used per analysis type: Trapping media were taken for analysis with the associated samples at sampling. In addition, trapping media associated with all remaining vessels were taken for analysis and replaced with fresh media at 14, 29, 46, 60, 74, 88 and 105 days after application.
- Sample storage before analysis: Aqueous samples and sediment extracts were stored at 5 ± 2°C prior to analysis.
DESCRIPTION OF CONTROL AND/OR BLANK TREATMENT PREPARATION
CONTROL AND BLANK SYSTEM
- same as test item
- Compartment:
- natural water / sediment: freshwater
- % Total extractable:
- 37.7
- % Non extractable:
- 8.2
- % CO2:
- 40.6
- % Other volatiles:
- 0
- % Recovery:
- 87.3
- Remarks on result:
- other: 105 d - Emperor Lake
- Compartment:
- natural water / sediment: freshwater
- % Total extractable:
- 11.7
- % Non extractable:
- 1.4
- % CO2:
- 0
- % Other volatiles:
- 0
- % Recovery:
- 93.3
- Remarks on result:
- other: 0 d - Emperor Lake
- Compartment:
- natural water / sediment: freshwater
- % Total extractable:
- 33.6
- % Non extractable:
- 7.3
- % CO2:
- 42
- % Other volatiles:
- 0
- % Recovery:
- 84.8
- Remarks on result:
- other: 105 d - Calwich Abbey Lake
- Compartment:
- natural water / sediment: freshwater
- % Total extractable:
- 14
- % Non extractable:
- 1.7
- % CO2:
- 0
- % Other volatiles:
- 0
- % Recovery:
- 96.4
- Remarks on result:
- other: 0 d - Calwich Abbey Lake
- Compartment:
- natural water / sediment: freshwater
- DT50:
- 25.2 d
- Type:
- not specified
- Temp.:
- 12 °C
- Remarks on result:
- other: Emperor Lake (whole system)
- Compartment:
- natural water / sediment: freshwater
- DT50:
- 19.6 d
- Type:
- not specified
- Temp.:
- 12 °C
- Remarks on result:
- other: Calwich Abbey Lake (whole system)
- Transformation products:
- no
- Remarks:
- Test item was degraded to five low level unidentified degradates (≤3.5% applied radioactivity), incorporated into bound (non-extractable) radioactivity and mineralized to carbon dioxide.
- Details on transformation products:
- Test item was degraded to five low level unidentified degradates (≤3.5% applied radioactivity), incorporated into bound (non-extractable) radioactivity and mineralized to carbon dioxide.
- Evaporation of parent compound:
- no
- Volatile metabolites:
- no
- Details on results:
- TEST CONDITIONS
- Aerobicity, moisture, temperature and other experimental conditions maintained throughout the study: Yes
- Anomalies or problems encountered (if yes): The temperature of the incubation room during the acclimatization phase of the preliminary experiment deviated from the acceptable range of 12 ± 2ºC on several occasions. This deviation occurred between the 10 May 2019 to 14 May 2019 (5 days). The maximum temperature reached was 14.9°C and the maximum duration of temperature excursion from the acceptable range was approximately 10 hours 16 minutes.
This deviation was not considered to have affected the outcome of the study.
MAJOR TRANSFORMATION PRODUCTS
- none
MINOR TRANSFORMATION PRODUCTS
Test item was degraded to two low level unidentified degradates (≤0.5% applied radioactivity), incorporated into bound (non-extractable) radioactivity and mineralized to carbon dioxide.
- Validity criteria:
- - Recoveries should range from 90% to 110% for labelled chemicals and from 70% to 110% for non-labelled chemicals.
- Observed value:
- the sum of radioactivity in the water layer, extractable and non-extractable radioactivity in sediment and volatile radioactivity) were between 81.0% to 95.5%
- Validity criteria fulfilled:
- yes
Reference
Microbiological Characterization of the Aquatic Sediments
Start of incubation
|
Calwich Abbey Lake |
Emperor Lake |
||
|
Sediment |
Water |
Sediment |
Water |
Aerobic bacteria |
6.95×105 |
1.15×102 |
3.60×106 |
4.85×102 |
Aerobic bacterial spores |
5.95×104 |
10 |
4.50×105 |
15 |
Anaerobic bacteria |
1.80×105 |
nm |
1.17×105 |
nm |
Anaerobic bacterial spores |
6.25×104 |
nm |
1.07×105 |
nm |
Actinomycetes |
3.85×104 |
<10 |
1.85×104 |
<10 |
Fungi |
3.65×104 |
<10 |
2.70×102 |
<10 |
End of incubation (untreated)
|
Calwich Abbey Lake |
Emperor Lake |
||
|
Sediment |
Water |
Sediment |
Water |
Aerobic bacteria |
2.90x105 |
4.40x102 |
8.90x105 |
80 |
Aerobic bacterial spores |
2.75x105 |
25 |
3.60x105 |
40 |
Anaerobic bacteria |
6.65x104 |
nm |
5.20x104 |
nm |
Anaerobic bacterial spores |
9.55x103 |
nm |
3.15x104 |
nm |
Actinomycetes |
5.85x103 |
<10 |
2.40x104 |
<10 |
Fungi |
2.00x103 |
<10 |
3.50x104 |
<10 |
End of incubation (solvent treated)
|
Calwich Abbey Lake |
Emperor Lake |
||
|
Sediment |
Water |
Sediment |
Water |
Aerobic bacteria |
3.30x105 |
50 |
1.05x106 |
1.05x102 |
Aerobic bacterial spores |
3.35x104 |
15 |
6.40x104 |
25 |
Anaerobic bacteria |
7.10x104 |
nm |
5.95x104 |
nm |
Anaerobic bacterial spores |
6.30x104 |
nm |
5.75x104 |
nm |
Actinomycetes |
6.05x103 |
<10 |
3.75x103 |
<10 |
Fungi |
1.75x103 |
<10 |
2.95x104 |
<10 |
Results are expressed as colony forming units/g
nm not measured
Mean Distribution and Recovery of Radioactivity in Calwich Abbey Lake Aquatic Sediment
|
Time after application (days) |
||||||
0 |
1 |
7 |
14 |
29 |
60 |
105 |
|
Water |
80.8 |
85.1 |
49.4 |
12.4 |
11.6 |
5.3 |
1.2 |
Sediment: |
|
|
|
|
|
|
|
Extract 1 |
9.7 |
5.2 |
21.3 |
32.7 |
31.9 |
25.2 |
20.0 |
Extract 2 |
4.3 |
2.1 |
9.2 |
13.5 |
11.8 |
11.2 |
9.3 |
Extract 3 |
- |
- |
- |
4.1 |
4.7 |
3.2 |
3.2 |
Extract 4 |
- |
- |
- |
- |
- |
- |
1.2 |
Vessel Rinse |
- |
- |
- |
- |
- |
- |
0.8 |
Total extractable |
14.0 |
7.2 |
30.4 |
50.2 |
48.4 |
39.5 |
33.6 |
Non-extractable |
1.7 |
0.6 |
6.0 |
9.7 |
7.7 |
9.2 |
7.3 |
Total in sediment |
14.7 |
8.2 |
36.4 |
59.9 |
56.0 |
48.7 |
40.8 |
Organic volatiles |
- |
nd |
nd |
nd |
nd |
0.1 |
nd |
CO2 |
- |
0.6 |
4.2 |
12.0 |
13.4 |
31.6 |
42.0 |
Total recovery |
96.4 |
93.4 |
90.0 |
84.3 |
81.0 |
85.6 |
84.8 |
Results are expressed as % applied radioactivity
- not applicable
nd not detected
Extracts 1 – 3 Tetrahydrofuran
Extract 4 Dichloromethane
Vessel rinse Tetrahydrofuran
Mean Distribution and Recovery of Radioactivity in Emperor Lake Aquatic Sediment
|
Time after application (days) |
||||||
0 |
1 |
7 |
14 |
29 |
60 |
105 |
|
Water |
80.2 |
85.9 |
42.5 |
23.0 |
13.4 |
4.2 |
0.3 |
Sediment: |
|
|
|
|
|
|
|
Extract 1 |
8.3 |
6.9 |
24.3 |
24.5 |
24.9 |
24.5 |
22.1 |
Extract 2 |
3.5 |
2.7 |
9.4 |
16.3 |
10.0 |
12.1 |
9.6 |
Extract 3 |
- |
- |
3.9 |
7.2 |
5.4 |
4.4 |
4.0 |
Extract 4 |
- |
- |
- |
- |
0.6 |
1.4 |
2.1 |
Vessel Rinse |
- |
- |
- |
- |
- |
- |
0.5 |
Total extractable |
11.7 |
9.5 |
37.6 |
49.0 |
40.8 |
42.3 |
37.7 |
Non-extractable |
1.4 |
nd |
6.8 |
9.3 |
9.7 |
9.8 |
8.2 |
Total in sediment |
13.1 |
9.5 |
44.4 |
58.2 |
50.4 |
52.1 |
46.0 |
Organic volatiles |
- |
nd |
nd |
nd |
nd |
nd |
nd |
CO2 |
- |
0.2 |
9.6 |
9.2 |
24.4 |
30.0 |
40.6 |
Total recovery |
93.3 |
95.6 |
96.5 |
90.4 |
88.2 |
86.2 |
87.3 |
Results are expressed as % applied radioactivity
- not applicable
nd not detected
Extracts 1 – 3 Tetrahydrofuran
Extract 4 Dichloromethane
Vessel rinse Tetrahydrofuran
Mean Proportions of Radioactive Components in Calwich Abbey Lake Aquatic Sediment
Water Phase
|
Time after application (days) |
||||||
0 |
1 |
7 |
14 |
29a |
60 |
105 |
|
Test item (Rt ~20.3 mins) |
77.2 |
79.7 |
44.6 |
2.3 |
- |
NA |
NA |
Polar (Rt ~2 - 4 mins) |
- |
- |
1.9 |
10.2 |
- |
||
Met 1 (Rt ~19.0 mins) |
- |
- |
1.3 |
- |
- |
|
|
Others |
3.6 |
5.4 |
1.7 |
- |
6.9 |
|
|
Sediment Phase
|
Time after application (days) |
||||||
0 |
1 |
7 |
14 |
29 |
60 |
105 |
|
Test item (Rt ~20.3 mins) |
13.2 |
7.2 |
26.9 |
42.8 |
42.8 |
38.7 |
31.7 |
Met 2 (Rt ~4.0 mins) |
- |
- |
0.3 |
1.2 |
1.0 |
0.3 |
- |
Met 4 (Rt ~12.0 mins) |
- |
- |
- |
0.6 |
- |
- |
- |
Met 4 (Rt ~19.0 mins) |
- |
- |
0.8 |
0.9 |
0.8 |
- |
- |
Others |
0.8 |
- |
2.0 |
4.8 |
3.9 |
0.5 |
0.7 |
Total System
|
Time after application (days) |
||||||
0 |
1 |
7 |
14 |
29 |
60 |
105 |
|
Test item (Rt ~20.3 mins) |
90.3 |
86.9 |
71.5 |
45.0 |
42.8 |
38.7 |
31.7 |
Polar (Rt ~2 - 4 mins) |
- |
- |
- |
10.2 |
- |
- |
- |
Met 2 (Rt ~ 4.0 mins) |
- |
- |
2.2 |
1.2 |
1.0 |
0.3 |
- |
Met 4 (Rt ~ 12.0 mins) |
- |
- |
- |
0.6 |
- |
- |
- |
Met 1 (Rt ~ 19.0 mins) |
- |
- |
2.0 |
0.9 |
0.8 |
- |
- |
Others |
4.4 |
5.4 |
4.2 |
4.8 |
10.8 |
0.5 |
0.7 |
Results are expressed as % applied radioactivity
(a) From one replicate analysis
Others Radioactivity distributed throughout regions of the chromatogram other than those specified and which did not contain any discrete radioactive components
- Not apparent or below the limit of detection
NA Not analyzed (insufficient radioactivity, <10% applied radioactivity)
Mean Proportions of Radioactive Components in Emperor Lake Aquatic Sediment
Water Phase
|
Time after application (days) |
||||||
0 |
1 |
7 |
14 |
29a |
60 |
105 |
|
Test item (Rt ~20.3 mins) |
77.4 |
81.1 |
39.7 |
19.4 |
8.0 |
NA |
NA |
Polar (Rt ~2 - 4 mins) |
- |
- |
- |
0.9 |
0.5 |
||
Met 5 (Rt ~6.0 mins) |
- |
- |
- |
- |
0.3 |
||
Met 1 (Rt ~19.0 mins) |
- |
- |
1.8 |
0.8 |
- |
||
Others | 2.9 | 4.8 | 1.0 | 2.1 | - |
Sediment Phase
|
Time after application (days) |
||||||
0 |
1 |
7 |
14 |
29 |
60 |
105 |
|
Test item (Rt ~20.3 mins) |
11.3 |
8.9 |
28.2 |
43.0 |
35.4 |
38.2 |
35.0 |
Polar (Rt ~2 - 4 mins) |
- |
- |
- |
- |
- |
- |
- |
Met 2 (Rt ~ 4.0 mins) |
- |
- |
0.5 |
0.7 |
0.9 |
0.6 |
- |
Met 3 (Rt ~ 15.0 mins) |
- |
- |
0.4 |
- |
- |
- |
- |
Met 1 (Rt ~ 19.0 mins) |
- |
- |
1.7 |
1.7 |
1.1 |
0.4 |
0.3 |
Others |
0.5 |
0.6 |
3.1 |
3.7 |
2.9 |
1.8 |
0.4 |
Total System
|
Time after application (days) |
||||||
0 |
1 |
7 |
14 |
29 |
60 |
105 |
|
Test item (Rt ~20.3 mins) |
88.6 |
90.0 |
67.9 |
62.4 |
43.4 |
38.2 |
35.0 |
Polar (Rt ~ 2 - 4 mins) |
- |
- |
- |
0.9 |
0.5 |
- |
- |
Met 2 (Rt ~ 4.0 mins) |
- |
- |
0.5 |
0.7 |
0.9 |
0.6 |
- |
Met 5 (Rt ~ 6.0 mins) |
- |
- |
- |
- |
0.3 |
- |
- |
Met 3 (Rt ~ 15.0 mins) |
- |
- |
0.4 |
- |
- |
0.4 |
0.3 |
Met 1 (Rt ~ 19.0 mins) |
- |
- |
3.5 |
2.6 |
1.1 |
1.8 |
0.4 |
Others |
2.0 |
5.4 |
4.1 |
5.7 |
2.9 |
|
|
Results are expressed as % applied radioactivity
Others
Radioactivity
distributed throughout regions of the chromatogram other than those
specified and
which
did not contain any discrete radioactive components
- Not apparent or below the limit of detection
a From one replicate analysis
NA Not Analyzed (insufficient radioactivity <10% applied radioactivity)
Kinetic Data for the Decline of Test item in Aquatic Sediment Systems
Test item
Aquatic sediment |
Compartment |
Kinetic model |
DT50 (days) |
DT90 (days) |
Calwich Abbey Lake |
water |
SFO |
5.1 |
16.8 |
Emperor Lake |
water |
DFOP |
5.2 |
>10,000 |
Calwich Abbey Lake |
sediment |
SFO |
176 |
583 |
Emperor Lake |
sediment |
SFO |
426 |
1410 |
Calwich Abbey Lake |
total system |
DFOP |
19.6 |
637 |
Emperor Lake |
total system |
DFOP |
25.2 |
911 |
(note knietic analysis data was not to GLP)
Kinetic models: SFO = single first order
FOMC = first order multi compartment
Description of key information
DT50 = 19.6 – 25.2 days (OECD 308)
Mineralisation 40.6 – 42% at 105 days (OECD 308)
Key value for chemical safety assessment
Additional information
One simulation test is available assessing the biodegradability of
(Z)-N-octadec-9-enylhexadecan-1-amide (CAS No. 16260-09-6) in water and
sediment (OECD 307). The fate of the substance was investigated in two
different water sediment systems; asandy
clay loam (Calwich Abbey Lake) and a silt loam(Emperor Lake). The
systems were incubated for 105 days in darkness. (Z)-N-octadec-9-enylhexadecan-1-amide
dissipated rapidly from the water of aquatic sediment systems with
estimated DT50 values of 5.1 days (Calwich Abbey Lake) and 5.2 days
(Emperor Lake). Decline in the total water/sediment system corresponded
to estimated DT50 values of 19.6 days (Calwich Abbey Lake) and 25.2 days
(Emperor Lake).
The substance was degraded to polar material (≤10.2% AR), five low level
unidentified transformation products (≤3.5% AR), incorporated into bound
(non-extractable) radioactivity and mineralized to carbon dioxide.A
mineralisation of the test substance of 40.6% after 105 days were
determined in theEmperor lake system. In the Calwich Abbey Lake
system the substance 42% of the substance were mineralized to CO2 by day
105. The polar material was shown to be comprised of multiple components
with each component representing <10% AR.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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