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EC number: 204-909-5 | CAS number: 128-80-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Data is from experimental lab report
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Principles of method if other than guideline:
- To evaluate the test substance for its ability to generate acute toxic effects in Cyprinus carpio during an exposure period of 96 hours
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Details on sampling:
- No data available
- Vehicle:
- not specified
- Details on test solutions:
- test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes.Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/i solution appeared to be a black turbid dispersion with a floating layer. The test solution was used as such.
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp
- Source: Zodiac, proefacc, "De Haar Vissen", L.U. Wageningen, the Netherlands.
- Length at study initiation : 2.25 ± 0.19 cm
- Weight at study initiation :0.23 ± 0.06 g
- Method of breeding: F1 from a single parent-pair bred in UV-treated water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- ISO-medium, hardness: 218 mg CaC03/l
- Test temperature:
- Between 19.0 and 21.6°C
- Dissolved oxygen:
- detailed in table no. 1
- Nominal and measured concentrations:
- 100 mg/I.
- Details on test conditions:
- -Test vessels: 6 litres, all-glass
-Loading: 0.23 g fish per litre, i.e. 5 fish per 5 litres of test medium. - Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: No mortality observed
- Sublethal observations / clinical signs:
MACROLEX
GREEN5B (mg/I)
Initial
number
of fish
Cumulative mortality
Total
Mortality
(%)
31/4h
24 h
48 h
72 h
96 h
Blank- control
5
0
0
01)
01)
01)
0
100
5
0#)
0*)
0*)
0*)
0*)
0
‘) :precipitate and floating layer observed
1): precipitate (probably fish faeces) observed
*) floating layer observed.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Under the conditions of the present test material induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/I during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/I, the regulatory limit concentration.
- Executive summary:
Test material was evaluated for its toxicity on Cyprinus carpio as per OECD 203 and European Economic Community (EEC). Test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/i solution appeared to be a black turbid dispersion with a floating layer. The test solution was used as such.
Under the conditions of the present test material induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/I during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/I, the regulatory limit concentration.Based on the LC50 value it can be concluded that test material is not hazardous to fish and can not be considered to be not classified as per CLP criteria.
Reference
Description of key information
Short-term toxicity to fish:
Test material was evaluated for its toxicity on Cyprinus carpio as per OECD 203 and European Economic Community (EEC). Test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/i solution appeared to be a black turbid dispersion with a floating layer. The test solution was used as such.
Under the conditions of the present test material induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/I during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/I, the regulatory limit concentration.Based on the LC50 value it can be concluded that test material is not hazardous to fish and can not be considered to be not classified as per CLP criteria.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Short-term toxicity to fish:
In an experimental study test material was evaluated for its toxicity on Cyprinus carpio as per OECD 203 and European Economic Community (EEC). Test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/i solution appeared to be a black turbid dispersion with a floating layer. The test solution was used as such.
Under the conditions of the present test material induced no mortality or other visible effects in carp at a nominal concentration of 100 mg/I during the 96-hour test period. Hence, the 96h-LC50 was above 100 mg/I, the regulatory limit concentration.
In another experimental report , study was conducted to assess the effect of test chemical on the mortality of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The stock solution was prepared by dissolving 0.1gm of the test substance in 100mL of potable water (passed through reverse osmosis system) and kept it for 24 hrs stirring. By giving half an hour of settling period filtered it . The nominal concentration were 0.45 mg/L, 0.9 mg/L, 1.8mg/L, 3.6mg/L, 7.2 mg/L. Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test item to various nominal test concentrations, LC0 was determine to be 7.2 mg/l . No mortalities were observed in any of the tested concentration.
Based on the LC50 value it can be concluded that test material is not hazardous to fish and can not be considered to be not classified as per CLP criteria.
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