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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1996

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The genotoxicity of lead acetate (0.5 to 2 mM) was examined in a HPRT mutation assay using Chinese hamster ovary K1 cells. Polymerase chain reaction (PCR) of HPRT cDNA and genomic DNA, as well as DNA sequencing, were used to characterize lead acetate-induced mutations.
GLP compliance:
not specified
Type of assay:
in vitro mammalian cell gene mutation test using the Hprt and xprt genes

Test material

Constituent 1
Chemical structure
Reference substance name:
Lead di(acetate)
EC Number:
206-104-4
EC Name:
Lead di(acetate)
Cas Number:
301-04-2
Molecular formula:
Pb(CH3COO)2
IUPAC Name:
lead di(acetate)
Test material form:
not specified

Results and discussion

Test results
Species / strain:
Chinese hamster Ovary (CHO)
Metabolic activation:
without
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
not examined
Positive controls validity:
not examined
Remarks on result:
other: Test system: all strains/cell types tested

Any other information on results incl. tables

Lead acetate linearly increased the mutation frequency in the HPRT assay at concentrations from 0.5 to 1.5 mM. Lead acetate exposure resulted in large deletions and single-base substitutions, with G-C base substitutions occurring 3.3-fold more frequently than A-T base substitutions.

Applicant's summary and conclusion

Conclusions:
Interpretation of results: positive
Executive summary:

The genotoxicity of lead acetate (0.5 to 2 mM) was examined in a HPRT mutation assay using Chinese hamster ovary K1 cells. Polymerase chain reaction (PCR) of HPRT cDNA and genomic DNA, as well as DNA sequencing, were used to characterize lead acetate-induced mutations. Lead acetate linearly increased the mutation frequency in the HPRT assay at concentrations from 0.5 to 1.5 mM. Lead acetate exposure resulted in large deletions and single-base substitutions, with G-C base substitutions occurring 3.3-fold more frequently than A-T base substitutions.