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Diss Factsheets
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EC number: 206-104-4 | CAS number: 301-04-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 1 996
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The genotoxicity of lead acetate (0.5 to 2 mM) was examined in a HPRT mutation assay using Chinese hamster ovary K1 cells. Polymerase chain reaction (PCR) of HPRT cDNA and genomic DNA, as well as DNA sequencing, were used to characterize lead acetate-induced mutations.
- GLP compliance:
- not specified
- Type of assay:
- in vitro mammalian cell gene mutation test using the Hprt and xprt genes
Test material
- Reference substance name:
- Lead di(acetate)
- EC Number:
- 206-104-4
- EC Name:
- Lead di(acetate)
- Cas Number:
- 301-04-2
- Molecular formula:
- Pb(CH3COO)2
- IUPAC Name:
- lead di(acetate)
- Test material form:
- not specified
Constituent 1
Results and discussion
Test results
- Species / strain:
- Chinese hamster Ovary (CHO)
- Metabolic activation:
- without
- Genotoxicity:
- positive
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- not examined
- Remarks on result:
- other: Test system: all strains/cell types tested
Any other information on results incl. tables
Lead acetate linearly increased the mutation frequency in the HPRT assay at concentrations from 0.5 to 1.5 mM. Lead acetate exposure resulted in large deletions and single-base substitutions, with G-C base substitutions occurring 3.3-fold more frequently than A-T base substitutions.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results: positive
- Executive summary:
The genotoxicity of lead acetate (0.5 to 2 mM) was examined in a HPRT mutation assay using Chinese hamster ovary K1 cells. Polymerase chain reaction (PCR) of HPRT cDNA and genomic DNA, as well as DNA sequencing, were used to characterize lead acetate-induced mutations. Lead acetate linearly increased the mutation frequency in the HPRT assay at concentrations from 0.5 to 1.5 mM. Lead acetate exposure resulted in large deletions and single-base substitutions, with G-C base substitutions occurring 3.3-fold more frequently than A-T base substitutions.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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