(E)-1,4-dibromobut-2-ene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

GLP guideline study with concentration control analysis. The analytically determined test concentrations at the end of the experiment were generally below the limit of detection. It is therefore expected that the effect concentrations relate to the hydrolysis product(s) of the test substance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(E)-1,4-dibromobut-2-ene

EC Number:

212-472-7

EC Name:

(E)-1,4-dibromobut-2-ene

Cas Number:

821-06-7

Molecular formula:

C4 H6 Br2

IUPAC Name:

1,4-dibromobut-2-ene

Details on test material:

- Name of test substance (as cited in study report): SPP100 PISA P2a
- Purity (%): 98.9
- Description: beige to light brown crystals

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

SAMPLING FOR ANALYSIS
- Samples for possible analysis were taken from all test concentrations and the control according to the schedule below.
- In addition, the filter used for preparation of test concentrations was retained for possible analysis of the residue
- Frequency: at t=0 h and t=48 h
- Volume: 6 ml from the approximate centre of the test vessels
- Storage: samples were stored in a freezer until analysis
- At the end of exposure period, the replicates were pooled at each concentration before sampling.

Test solutions

Details on test solutions:

PREPARATION OF TEST SOLUTIONS
- The test substance consists of beige to light brown crystals and is not completely soluble in test medium at the loading rates prepared.
- Preparation of test solutions started with a loading rate of 100 mg/L. After a 48-hour stirring period the obtained aqueous mixture was filtered through a 0.45 µm membrane filter (rc 55, Whatman). pH of the filtrate was adjusted from 3.2/3.3 to 6.5/6.6 using 1 N NaOH. The lower test concentrations were prepared by subsequent dilutions of the filtrate in test medium. The final test solutions were all clear and colourless.
- Subsequently, a loading rate of 100 mg/L was prepared for a third experiment. After 48 hours of stirring this loading rate was observed to be completely dissolved. pH was not measured and not adjusted. Instead the solution was directly sampled and analysed.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Species: Daphnia magna STRAUS, 1980; at least third generation, obtained by acyclical parthenogenesis under specified breeding conditions
- Source: In-house laboratory culture
- Validity of batch: Daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress
- Breeding: start of each batch with newborn daphnids, i.e. less than 3 days old, by placing about 250 of them into 5 litres of medium in an all-glass culture vessel
- Maximum age of cultures: 4 weeks
- Renewal of cultures: after 7 days of cultivation half of the medium twice a week
- Temperature of medium: 18 - 22°C
- Feeding: daily with suspension of fresh algae
- Medium: M7, as prescribed by Dr. Elendt-Schneider

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

18.7 - 20.5°C

pH:

6.7 - 7.3

Dissolved oxygen:

8.9 - 9.1 mg/L

Nominal and measured concentrations:

Nominal test concentrations: 2.2, 4.6, 10, 22 and 46% of a filtered solution prepared at a loading rate of 100 mg/L

Details on test conditions:

- Test vessels: 100 ml, all-glass
- Test medium: adjusted ISO medium
- Number of daphnids: 20 per concentration
- Replicates: 4 per concentration
- Loading: 5 per vessel containing 80 ml of test solution
- Light: 16 h photoperiod daily
- Feeding: none
- Aeration: none
- Introduction of daphnids: within 35 min after preparation of test solutions

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 (48 h) 0.36 mg/L