Registration Dossier
Registration Dossier
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EC number: 436-000-6 | CAS number: 159010-67-0
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From April 23rd to 26th, 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Version / remarks:
- 1984
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Blue REg 6080
- IUPAC Name:
- Blue REg 6080
Constituent 1
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 19-20 °C
- pH:
- 8.2-8.7 (test item)
8-8.7 (control) - Dissolved oxygen:
- 7.2-8.1 mg/l
7.4-8 mg/l - Nominal and measured concentrations:
- Nominal concentrations: 10, 32, 100, 320, 1000 mg/l
- Details on test conditions:
- Continuous aeration during 3-h test period.
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol at 5, 16 and 50 mg/l
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 000 mg/L
- Details on results:
- Up to 1000 mg/l, test item had no significant inhibitory effect (< 15 %) on the respiration rate of activated sludge after the incubation period of 3 hours. Based ont the physical appearance of test suspensions. the saturation concentration (equilibrium) under test conditions was < 10 mg/l and resulted in less than 15 % inhibition after 3 hours contact time. Thus, EC50 is higher than the water solubility limit of test itme, or higher then nominal 1000 mg/l; NOECof test item was at least at the limit of water solubility or at least at nominal 1000 mg/l.
- Results with reference substance (positive control):
- Test results confirmed the suitability of the activated sludge and method used.
Applicant's summary and conclusion
- Conclusions:
- NOEC (3h) = 1000 mg/l (nominal concentration)
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