3-(methylamino)propane-1,2-diol

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Not stated

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted to internationally accepted guidelines (OECD 302 B). Report also contains QA statement and has been conducted according to GLP. Some details on the test substance were missing, eg purity, lot number.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-(methylamino)propane-1,2-diol

EC Number:

254-809-0

EC Name:

3-(methylamino)propane-1,2-diol

Cas Number:

40137-22-2

Molecular formula:

C4H11NO2

IUPAC Name:

3-(methylamino)propane-1,2-diol

Constituent 2

Reference substance name:

methylaminopropandiol

IUPAC Name:

methylaminopropandiol

Details on test material:

- Name of test material (as cited in study report): methylaminopropandiol (MAPD)
- Physical state: Liquid
- Analytical purity: Not stated
- Lot/batch No.: Not stated
- Storage condition of test material: 5°C in the dark

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): secondary settlement tank from Rungsted sewage plant. This plant does not receive industrial sewage. The sludge was collected immediately prior to test set up.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

80 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: Not stated
- Test temperature: Not stated
- pH adjusted: yes (pH was daily adjusted to about 7.0 with 1N sodium hydroxide)
- Aeration of dilution water: Not stated
- Suspended solids concentration: 1.1 g solid per 1 mL (1g) test solution
- Continuous darkness: Not stated

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Not stated
- Test performed in open system: Not stated

SAMPLING
- Sampling frequency: 0 and 3 hours from start then on days 7, 14, 20 and 28.
- Sampling method: Not stated
- Sterility check if applicable: Not stated
- Sample storage before analysis: samples filtered through 0.45 um filter (cellulose nitrate) and frozen prior to analysis

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes (microbial activity was stopped by applying 100 mg Mercury chloride per litre).

Reference substance

Reference substance:

diethylene glycol

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

94

Sampling time:

28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

inherently biodegradable

Conclusions:

The test compound MAPD was in the test ultimately degradable within 28 days .
MAPD was degraded by 94 percent of the initial concentration.