Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 270-478-5 | CAS number: 68442-22-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1983
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Protocol that complies with scientifically accepted methods, and is sufficiently detailed.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Section 1500.3 – Federal Hazardous Substance Act Regulations – 16 CFR.
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- Phosphorodithioic acid, mixed O,O-bis(2-ethylhexyl and iso-Bu) esters, zinc salts
- EC Number:
- 270-478-5
- EC Name:
- Phosphorodithioic acid, mixed O,O-bis(2-ethylhexyl and iso-Bu) esters, zinc salts
- Cas Number:
- 68442-22-8
- Molecular formula:
- Too complex
- IUPAC Name:
- Phosphorodithioic acid, mixed O,O-bis(2-ethylhexyl and iso-Bu) esters, zinc salts
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
Source: Ace Animal, Inc. Boyertown, PA.
Age at study initiation: no data available.
Weight at study initiation: average weight between 200~300 g.
Fasting period before study: Feed was withheld overnight prior to dosing.
Housing: the animals were housed and maintained in accordance with standards set forth in the Guide for Care and Use of Laboratory Animals (DHEW publication No. 80-23). 5 rats/cage by sex.
Diet: Wayne Lab-Blox, provided ad libitum..
Water: tap water available ad libitum.
Acclimation period: acclimated to the laboratory for an appropriate time prior to dosing.
ENVIRONMENTAL CONDITIONS
Temperature (°C): 18.3~22.8 °C
Humidity (%): 45% ~ 55%
Air changes: no data available.
Photoperiod: 12 h light/dark cycle.
IN-LIFE DATES: From: 03/02/1983 To: 04/13/1983.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- 2000 and 5000 mg/kg bw
- No. of animals per sex per dose:
- 5/sex/dose
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed frequently on the day of dosage and twice daily thereafter (morning and afternoon). Individual weights were recorded on the day of dosage, weekly thereafter and prior to sacrifice. Individual weights were recorded on the day of dosage, and average groups weights were recorded at 7 and 14 days after dosing.
- Necropsy of survivors performed: gross necropsies were performed on all animals that either died during the 14 day observation period or on surviving animals that were sacrificed at the conclusion of the 14 day observation period. - Statistics:
- no data available
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 - < 5 000 mg/kg bw
- Mortality:
- 5000 mg/kg
Male: Number of animals: 5; Number of deaths: 5
Female: Number of animals: 5; Number of deaths: 5
2000 mg/kg
Male: Number of animals: 5; Number of deaths: 0
Female: Number of animals: 5; Number of deaths: 2 - Clinical signs:
- other: In the 2000 mg/kg dose group, all survival animals appeared normal.
- Gross pathology:
- No gross abnormalities were noted in all animals (either found dead during the study or in the animals necropsied at the conclusion of the study).
Any other information on results incl. tables
Table 1. Results
Dose Level (mg/kg) |
5000 |
2000 |
|||
Sex |
Male |
Female |
Male |
Female |
|
Average Body Weight (g) |
Initial |
261 |
211 |
222 |
206 |
7 d |
─ |
─ |
259 |
218 |
|
14 d |
─ |
─ |
313 |
231 |
|
Mortality (No. death/No. dosed) |
5/5 |
5/5 |
0/5 |
2/5 |
Table 2: Observations:
Sex |
Dose (mg/kg) |
Time after dosing |
Observation |
Male |
2000 |
Up to 14 d |
All animals appeared normal. |
5000 |
4 h 24 h
48 h
72 h |
5/5 appeared normal 2/5 found dead; 3/5 appeared ruffled and exhibited depressed activity 1/5 found dead, 2/5 appeared ruffled and exhibited diarrhea. 2/5 found dead. |
|
Female |
2000 mg/kg |
4 h 24 h
48 h 72 h – 14 d |
5/5 appeared ruffled 1/5 appeared ruffled; 3/5 appeared ruffled and exhibited depressed activity 1/5 found dead; 3/5 appeared normal |
5000 mg/kg |
4 h 24 h
48 h |
5/5 appeared normal 2/5 found dead; 3/5 appeared ruffled and exhibited depressed activity 3/5 found dead. |
No gross abnormalities were observed.
Applicant's summary and conclusion
- Interpretation of results:
- Category 5 based on GHS criteria
- Remarks:
- Migrated information
- Conclusions:
- The test article, when administered as received to male/female Sprague-Dawley rats, had an acute oral LD50 of greater than 2000 mg/kg bodyweight but less than 5000
- Executive summary:
In an acute oral toxicity study, 2 groups fasted Sprague-Dawley strain Albino rats (five male and five female) were given a single oral dose of undiluted test material at a dose level of 2000 and 5000 mg/kg bw and observed for14 days.
All animals died in the high dose group. In the low dose group, 2/5 female died on day 2; all the survived animals appeared normal. No gross abnormalities were noted in all animals (either found dead during the study or in the animals necropsied at the conclusion of the study). All animals showed expected bodyweight gain during the study period.
The oral LD50 value of test material in rats of both sexes has been determined to be greater than 2000 mg/kg bodyweight but less than 5000 mg/kg bodyweight.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)