Triphenylphosphine

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: screening test: discrepancy between documented test parameters and standard methods, but scientifically acceptable

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1971

Report date:

1971

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

- detection of skin irritation according to BASF-internal standards
- Principle of test: The test substance was applied to the back (A) or ear skin (B) of rabbits. Signs of local irritation and intoxication were recorded 24 hours and 8 days after application.
A) Back skin: The test substance was administered as a 2.5 x 2.5 cm patch loaded with a 50% alcoholic suspension (quanitity not specified) to the shaved skin of 2 rabbits for defined time intervals of either 1 min, 5 min, 15 min or 20 h. After the short exposure times of 1 - 15 min the skin was washed, no washing was conducted after the 20 hour exposure.
B) Ear: Cotton-wool soaked with approx. 2 g of the 50% alcoholic suspension (equivalent to 1g test substance) were applied to the pinna and held in place gauze bandages for 20 h.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Triphenylphosphine

EC Number:

210-036-0

EC Name:

Triphenylphosphine

Cas Number:

603-35-0

Molecular formula:

C18H15P

IUPAC Name:

triphenylphosphine

Test material form:

solid: crystalline

Specific details on test material used for the study:

- Purity: 99%.

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: preparation of a 50% alcoholic suspension

FORM AS APPLIED IN THE TEST (if different from that of starting material): suspension

Test animals

Species:

rabbit

Strain:

Vienna White

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: purchased freely.
- Weight at study initiation: 3.1 kg.
- Diet: ad libitum.
- Water: ad libitum.

Test system

Type of coverage:

occlusive

Preparation of test site:

shaved

Vehicle:

other: alcoholic suspension

Controls:

no

Amount / concentration applied:

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): not specified for apllication on back skin; 2g 50% alcoholic preparation on rabbit ear.

Duration of treatment / exposure:

A) Back skin: 1 min, 5 min, 15 min or 20 hours
B) Ear: 20 hours

Observation period:

8 days

Number of animals:

2

Details on study design:

A) Back Skin:

TEST SITE
- Area of exposure: 2.5 x 2.5 cm.
- Type of wrap if used: patch loaded with the suspension.

REMOVAL OF TEST SUBSTANCE
- Washing (if done): Skin was washed with undiluted Lutrol and 50% Lutrol/Water solution.
- Time after start of exposure: Washing conducted after 1, 5, 15 min exposure but not after 20 h exposure.

OBSERVATION TIME POINTS
(indicate if minutes, hours or days): Text indicates readings 0, 1, 2, and 7 days after removal, however, results are given for 1 and 8 days.

SCORING SYSTEM:
- Method of calculation: grading system for reddening and edema (no effect, slight, marked).
---------------------------------------------------------
A) Ear:

TEST SITE
- Area of exposure: soaked Cotton-wool (no further details).
- Type of wrap if used: gauze bandages to hold cotton wool in place.

OBSERVATION TIME POINTS
(indicate if minutes, hours or days): Text indicates readings 0, 1, 2, and 7 days after removal, however, results are given for 1 and 8 days.

SCORING SYSTEM:
- Method of calculation: grading system for reddening and edema (no effect, slight, marked).

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Remarks:

Back skin

Basis:

other: 1 of 2 animals

Time point:

24 h

Score:

2

Reversibility:

fully reversible within: 8 days

Remarks on result:

other:

Remarks:

slight reddening (R+)

Results 2

Irritation parameter:

erythema score

Remarks:

Ear

Basis:

other: 1 of 2 animals

Time point:

24 h

Score:

2

Reversibility:

fully reversible within: 8 days

Remarks on result:

other:

Remarks:

slight reddening (R+)

Irritant / corrosive response data:

A) Back skin: After exposures of up to 15 min, no skin reactions were observed. 20-h exposure caused slight reddening at the application site in 1/2 animals 24 h after removal of the patch, which was fully reversible within 8 days.
B) Ear: 20-h exposure caused slight reddening at the application site in 1/2 animals 24 h after removal of the bandage, which was fully reversible within 8 days.

Other effects:

No systemic clinical effects were observed.

Any other information on results incl. tables

Table 1: Local irritation

Application site	Application time	Number and Sex  of animals	findings
			after 24 hours	after 8 days
Back Skin	1 min	1 female 1 male	- -	- -
	5 min	1 female 1 male	- -	- -
	15 min	1 female 1 male	- -	- -
	20 h	1 female 1 male	- R+	- -
Ear	20 h	1 female 1 male	- R+	- -

R+ = slight reddening

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

A 20-hour application of an 50% alcoholic TS prepration induced a slight reddening of the back skin and ears of 1/2 rabbits, which was fully reversible within 8 days. Therefore, the TS is considered to be not irritating.

Executive summary:

The irritation/corrosion potential of the test substance was tested according to BASF-internal standards.

A 50% alcoholic preparation of the test substance was applied occluded to the back (1 min, 5 min, 15 min and 20 hours) and ear skin (20 hours) of two rabbits. Signs of local irritation and intoxication were recorded 24 hours and 8 days after application.

A exposure of rabbit back skin for up to 15 min produced no evidence of skin irritation. A 20-hour application induced a slight reddening of the back and ear skin of 1/2 rabbits (male), which was fully reversible within 8 days.

Therefore, the test substance is considered to be not irritating.