Tetrakis(phenylmethyl)thioperoxydi(carbothioamide)

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

May 27, 1987 to July 3, 1987

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

Guideline study performed in compliance with GLP, available as unpublished report, no restrictions, fully adequate for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Tetrakis(phenylmethyl)thioperoxydi(carbothioamide)

EC Number:

404-310-0

EC Name:

Tetrakis(phenylmethyl)thioperoxydi(carbothioamide)

Cas Number:

10591-85-2

Molecular formula:

C30H28N2S4

IUPAC Name:

N,N-dibenzyl[(dibenzylcarbamothioyl)disulfanyl]carbothioamide

Specific details on test material used for the study:

- Name of test material (as cited in study report): Tetrabenzylthiuramdisulphide (TBeTD; same as TBzTD)
- Substance type: Mono-constituent substance
- Physical state: Off-white powder
- Purity test date: > 95%, technically pure
- Lot/batch No.: G 1542

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538

Metabolic activation:

with and without

Metabolic activation system:

Arochlor-induced rat liver

Test concentrations with justification for top dose:

Concentration range in the main test (with metabolic activation): CA 24.69 ... CA 2000 µg/plate
Concentration range in the main test (without metabolic activation): CA 24.69 ... CA 2000 µg/plate

Vehicle / solvent:

Solvent: DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

Remarks:

DMSO

True negative controls:

no

Positive controls:

yes

Positive control substance:

9-aminoacridine

2-nitrofluorene

sodium azide

other: 2-aminoanthracene

Details on test system and experimental conditions:

Concentration of the test substance resulting in precipitation: 2000 µg/plate

Results and discussion

Test results

Key result

Species / strain:

S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not applicable

Positive controls validity:

valid

Additional information on results:

No signs of toxicity at any concentration tested.

No reduction of background bacterial lawn at any concentration.

Positive controls gave the expected strong increase in the number of S9+ and S9- revertants.

Applicant's summary and conclusion

Conclusions:

Tetrabenzylthiuram disulfide (TBzTD) did not show mutagenic activity in any of the strains of Salmonella typhimurium, either in the absence or in the presence of the S-9 mix.

Executive summary:

1. Tetrabenzylthiuram disulfide was examined for mutagenic activity in the Ames test using the histidine requiring Salmonella typhimurium mutants TA 1535, TA 1537, TA 1538, TA 98 and TA 100, and a liver microsome fraction of Aroclor-induced rats for metabolic activation (S-9 mix).

2. It was concluded that tetrabenzylthiuram disulfide did not show mutagenic activity in any of the strains of ~ typhimurium, either in the absence or in the presence of the S-9 mix.