Methanesulphonic acid

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation / corrosion

Remarks:

in vitro

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Meets generally accepted scientific standards.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Reference 2

Reference Type:

publication

Title:

HPVIS, IUCLID Data Set for Methanesulphonic acid, CAS no. 75-75-2

Author:

US-EPA

Year:

2003

Bibliographic source:

HPV Chemicals Challenge Program OPPT NCIC 201-14249B

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 435 (In Vitro Membrane Barrier Test Method for Skin Corrosion)

Principles of method if other than guideline:

Corrositex continuous time monitor assay was used to detect the corrosive potential of the test substance. This assay is used as a standardized and quantitative in-vitro corrosivity test. The test is based on the time that is required for the test sample to pass through a biobarrier membrane and produce a change in the Chemical Detection System. Both anhydrous methane sulfonic acid (MSA) and 70 % MSA were tested.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methanesulphonic acid

EC Number:

200-898-6

EC Name:

Methanesulphonic acid

Cas Number:

75-75-2

Molecular formula:

CH4O3S

IUPAC Name:

methanesulfonic acid

Details on test material:

- Name of test material (as cited in study report): methane sulfonic acid
- Physical state: anhydrous fluid and aqueous solution
- Composition of test material, percentage of components: undiluted (anhydrous MSA) and 70 % MSA in water
- Lot/batch No.: L06F
- Other: Source: Elf Atochem

Test animals

Species:

other: in vitro Corrositex Assay

Test system

Type of coverage:

other: not applicable

Vehicle:

other: unchanged (no vehicle) for anhydrous MSA and water for 70% MSA

Amount / concentration applied:

TEST MATERIAL
- Concentration (if solution): anhydrous and 70 % MSA

Duration of treatment / exposure:

4 hours, single exposure

Observation period:

4 hours

Number of animals:

not applicable

Details on study design:

Corrositex Assay:
- The Corrositex Biobarrier Membranes consist of a reconstituted collagen matrix was placed onto the CDS solution contained in test vials. The biobarrier membrane batch was qualified by testing the positive control (NaOH), a blank vial serves as negative control. Prior to testing a qualification screen and a categorization screen are performed to validate if the assay is appropriate for testing of the substance and to categorize the test substance as weak or strong acid/base, respectively.
- For testing, 500 µl of the test substance are placed on the membrane, 4 replicates are prepared and tested at the same time.
- Once the test substance is placed on the membrane disc, the vials are continuously observed for the first 10 minutes and then at approximately 5-minute intervals for up to 4 hours. The elapsed time from test substance application until CDS colour change is recorded.


SCORING SYSTEM:
Time required for CDS (chemical detection system) colour change (minutes):
0 to 3 min: corrosive (Packing Group I, GHS Cat 1A)
> 3 to 60 min: corrosive (Packing Group II, GHS Cat 1B)
> 60 to 240 min: corrosive (Packing Group III, GHS Cat 1C)
> 240 min: non-corrosive

Results and discussion

In vivo

Irritant / corrosive response data:

Average break-through time (mean of 4 determinations): 7 min 02 s (70 % methane sulfonic acid) and 3 min 43 s (anhydrous).

Any other information on results incl. tables

Results corrosivity test:

Test Article		Break Through Time (min:sec)
Sponsor's designation	MA Number	Vial 1	Vial 2	Vial 3	Vial 4	Mean	Packing group	pH*
70 % Methane Sulfonic Acid	95BW22	7:20	7:00	6:50	6:57	7:02	II	0
Methane Sulfonic Acid	95BW23	3:50	3:38	3:50	3:35	3:43	II	0
NaOH	-	12:23	NA	NA	NA	12:23	NA	NA

*100 µl/ml solution in deionized water NA - Not Applicable