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EC number: 238-034-5 | CAS number: 14177-55-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- January to April 1986
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Meets generally accepted scientific standards with acceptable restrictions
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Guinea pig mazimization test
- Principles of method if other than guideline:
- According to the Guinea pig mazimization test
- GLP compliance:
- yes
- Type of study:
- guinea pig maximisation test
Test material
- Reference substance name:
- Nickel sulphate
- EC Number:
- 232-104-9
- EC Name:
- Nickel sulphate
- Cas Number:
- 7786-81-4
- IUPAC Name:
- nickel(2+) sulfate
- Details on test material:
- - Name of test material (as cited in study report): Nickel sulphate (NiSO4) hexahydrate
- Lot No. JM07021EL
- Storage: room temperature
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- Hartley
- Sex:
- female
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc., Wilmington, MA, USA.
- Age at study initiation: young adult
- Weight at study initiation: 345-350 g
- Housing: individually housed in wire mesh cages
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: 5 days
ENVIRONMENTAL CONDITIONS: controlled, details not reported
IN-LIFE DATES: not reported
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- intradermal
- Vehicle:
- propylene glycol
- Concentration / amount:
- 0.1 ml of 1.0% w/v NiSO4 in distilled water
0.1 ml of Freunds Complete adjuvant (FCA)
0.1 ml of 1.0% w/w NiSO4 in 50% w/v (FCA)
0.3 ml of 5.0% NiSO4 in distilled water for 48 hours
Challengeopen allclose all
- Route:
- intradermal
- Vehicle:
- propylene glycol
- Concentration / amount:
- 0.1 ml of 1.0% w/v NiSO4 in distilled water
0.1 ml of Freunds Complete adjuvant (FCA)
0.1 ml of 1.0% w/w NiSO4 in 50% w/v (FCA)
0.3 ml of 5.0% NiSO4 in distilled water for 48 hours
- No. of animals per dose:
- 10 per dose
- Details on study design:
- MAIN STUDY
A. INDUCTION EXPOSURE
0.1 ml of 1.0% w/v NiSO4 in distilled water
0.1 ml of Freunds Complete adjuvant (FCA)
0.1 ml of 1.0% w/w NiSO4 in 50% w/v (FCA)
0.3 ml of 5.0% NiSO4 in distilled water and occluded for 48 hours
B. CHALLENGE EXPOSURE
Challenge performed on both flanks and ventral surface and occluded for 24 hours at concentrations of
0.2 ml of 1.0% w/v NiSO4 in distilled water
Erythema and edema scored according to Draize et al. 1944. - Challenge controls:
- Induction: 0.1 ml of propylene glycol, 0.1 ml of FCA in distilled water, or 0.1 ml of 1.0% w/v proylene glycol in 50% w/v FCA.
Challenge: 0.2 ml of propylene glycol - Positive control substance(s):
- yes
- Remarks:
- 1-chloro-2,4-dinitrobenzene
Study design: in vivo (LLNA)
- Vehicle:
- not specified
- Concentration:
- not applicable
- No. of animals per dose:
- not applicable
- Details on study design:
- not applicable
- Positive control substance(s):
- not specified
- Statistics:
- Mantel-Haenszel procedure
Results and discussion
- Positive control results:
- 1-chloro-2,4-dinitrobenzene did cause dermal contact sensitization in female albino guinea pigs.
In vivo (non-LLNA)
Resultsopen allclose all
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 0.2 ml of 1.0% w/v nickel sulfate in distilled water
- No. with + reactions:
- 10
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 0.2 ml of 1.0% w/v nickel sulfate in distilled water. No with. + reactions: 10.0. Total no. in groups: 10.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 0.2 ml of 1.0% w/v nickel sulfate in distilled water
- No. with + reactions:
- 10
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 0.2 ml of 1.0% w/v nickel sulfate in distilled water. No with. + reactions: 10.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- negative control
- Dose level:
- negative control
- No. with + reactions:
- 1
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: negative control. Dose level: negative control. No with. + reactions: 1.0. Total no. in groups: 10.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- negative control
- Dose level:
- negative control
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: negative control. Dose level: negative control. No with. + reactions: 0.0. Total no. in groups: 10.0.
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- positive control
- Dose level:
- positive control
- No. with + reactions:
- 6
- Total no. in group:
- 6
- Remarks on result:
- other: Reading: 1st reading. . Hours after challenge: 24.0. Group: positive control. Dose level: positive control. No with. + reactions: 6.0. Total no. in groups: 6.0.
- Reading:
- 2nd reading
- Hours after challenge:
- 48
- Group:
- positive control
- Dose level:
- positive control
- No. with + reactions:
- 6
- Total no. in group:
- 6
- Remarks on result:
- other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: positive control. Dose level: positive control. No with. + reactions: 6.0. Total no. in groups: 6.0.
In vivo (LLNA)
Resultsopen allclose all
- Parameter:
- SI
- Remarks on result:
- other: not applicable
- Parameter:
- other: disintegrations per minute (DPM)
- Remarks on result:
- other: not applicable
Any other information on results incl. tables
Mean erythema score at 24 and 48 hours for NiSO4 were 1.1 and 1.0, respectively
Applicant's summary and conclusion
- Conclusions:
- Nickel sulfate did cause dermal contact sensitization in female albino guinea pigs.
Mean erythema score at 24 and 48 hours for NiSO4 were 1.1 and 1.0, respectively. - Executive summary:
STUDY RATED BY AN INDEPENDENT REVIEWER.
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