Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-826-4 | CAS number: 127-19-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study is comparable to OECD Guideline 402 with acceptable restrictions (partly limited documentation, e.g. no details on test substance; no data about systemic clinical signs; 4 animals per dose used, 5 per dose recommended in the Guideline).
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 955
- Report date:
- 1955
- Reference Type:
- publication
- Title:
- Range-finding toxicity data: List VI
- Author:
- Smyth HF, Carpenter CP, Weil CS, Pozzani UC, Striegel JA
- Year:
- 1 962
- Bibliographic source:
- Am Ind Hyg Assoc J 23: 95-107
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- N,N-dimethylacetamide
- EC Number:
- 204-826-4
- EC Name:
- N,N-dimethylacetamide
- Cas Number:
- 127-19-5
- Molecular formula:
- C4H9NO
- IUPAC Name:
- N,N-dimethylacetamide
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material: dimethylacetamide (DMAC)
No details available.
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Weight at study initiation: ca. 2.5 kg
- Age at study initiation: 3-5 months old
No further details available.
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Details on dermal exposure:
- The fur was closely clipped over the entire trunk; the dose was retained under an impervious plastic film; animals were immobilized during the exposure period.
- Duration of exposure:
- 24 h
- Doses:
- 1260, 2520, 5000 µL/kg bw (corresponding to 1180, 2370, 4700 mg/kg bw)
- No. of animals per sex per dose:
- 4 rabbits
- Control animals:
- no
- Details on study design:
- Dosages greater than 20 mL/kg bw could not be retained in contact with the skin; after 24 h contact the impervious plastic film was removed.
Post exposure observation period: 14 days - Statistics:
- The LD50 value and its fiducial range were estimated by the method of Thompson (Bact. Rev. 11 :115, 1947) using the tables of Weil (Biometrics 8 :249, 1952).
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 2 240 other: µL/kg bw
- 95% CL:
- >= 1 230 - <= 4 110
- Remarks on result:
- other: corresponding to 2100 mg/kg bw
- Mortality:
- 5 mL/kg bw: 4/4 rabbits died. One rabbit died within 60 minutes, the other 3 animals died after 1-3 days.
2.5 mL/kg bw: 2/4 rabbits died 2 and 5 days after application.
1.26 mL/kg bw: 1/4 rabbits died after 3 days. - Clinical signs:
- other: Local effects were found noted including erythema, necrosis and desquamation. There are no data about systemic effects.
- Gross pathology:
- Several rabbits that died had lung haemorrhage and speckled or pitted kidney surface; in one animal of the highest dose level, there was blood in the peritoneum.
- Other findings:
- No
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The dermal LD50 of DMAC was determined to be 2100 mg/kg bw in male rabbits.
- Executive summary:
Groups of 4 male New Zealand rabbits were dermally exposed to doses of 1.26, 2.5, or 5 mL/kg bw. The post exposure observation period was 14 days. One out of 4, 2/4, or 4/4 rabbits died after exposure, respectively. Local effects like erythema, necrosis, desquamation were recorded (no data about systemic effects). Necropsy of dead animals revealed lung hemorrhage, speckled or pitted kidney surface and blood in peritoneum. The dermal LD50 in male rabbits was 2240 µL/kg bw corresponding to 2100 mg/kg bw.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)