2-chloro-4-nitrotoluene

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1988

Report date:

1988

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

- no strains with an AT base pair at the primary reversion site (E. coli WP2 strains or S. typhimurium TA 102) were used to detect cross-linking mutagens

GLP compliance:

yes

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

2-chloro-4-nitrotoluene

EC Number:

204-501-7

EC Name:

2-chloro-4-nitrotoluene

Cas Number:

121-86-8

Molecular formula:

C7H6ClNO2

IUPAC Name:

2-chloro-1-methyl-4-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): EXT 8808 2-Chloro-4-Nitrotoluene
- Purity: no data

Method

Species / strain

Species / strain / cell type:

S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538

Additional strain / cell type characteristics:

not applicable

Metabolic activation:

with and without

Metabolic activation system:

S9 mix from Aroclor 1254 induced rat livers

Test concentrations with justification for top dose:

10, 100, 250, 500, 750 µg/plate

Vehicle / solvent:

Solvent used: DMSO

Controls

Untreated negative controls:

no

Negative solvent / vehicle controls:

yes

True negative controls:

no

Positive controls:

yes

Positive control substance:

other: sodium azide, 9-amino-acridine, 2-nitrofluorene and 2-aminofluorene

Details on test system and experimental conditions:

The plate incorporation method was used.

Evaluation criteria:

A mutagenic effect is significant when, at least in one strain, the number of revertants histidine+ is twice higher than that found in the control.

Results and discussion

Test results

Species / strain:

S. typhimurium, other: TA 98, TA 100, TA 1535, TA 1537, TA 1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

no cytotoxicity

Vehicle controls validity:

valid

Untreated negative controls validity:

not examined

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

A bacteriostatic effect was observed in TA 98 and TA 100 with and without metabolic activation at the concentrations of 1000 and 5000 µg/plate. At the other concentrations no bacteriostatic effect was observed.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Executive summary:

In the Ames test with the S. typhimurium strains TA 98, TA 100, TA 1535, TA 1537 and TA 1538 EXT 8808 2-chloro-4-nitrotoluene revealed no mutagenic activity in the absence and in the presence of a metabolic activation system (OECD TG 471).