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Diss Factsheets
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EC number: 226-789-3 | CAS number: 5468-75-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Year of publication: 1981
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- no
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The sludge used by the different laboratories (5 laboratories) was domestic for 4 laboratories. One laboratory however used industrial sludge.
Preparation and amount of sludge used was as specified by OECD 209. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 +- 2°C
- pH:
- pH: between 7 and 8
- Nominal and measured concentrations:
- Limit test: 100 mg/L nominal concentration
- Details on test conditions:
- Identical to OECD-guideline 209, as this publication was the basis for the development of the guideline.
For those chemicals whose respiration rate was at least 80% of the control, the IC50 (concentration for 50% inhibition of respiration rate) is recorded as >100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The respiration rate for the submission substance at a concentration of 100 mg/L was >= 80% of the control samples without test item.
- Results with reference substance (positive control):
- Concentration of 3,5-dichlorophenol [mg/L] :: mean inhibition [%]:
100 :: 90%
50 :: 91%
25 :: 78%
10 :: 36%
1 :: 6% - Validity criteria fulfilled:
- not specified
- Remarks:
- However, as validity criteria for guideline OECD 209 were specified, these criteria were probably fulfilled for the reported test results.
- Conclusions:
- In a publication regarding the development of a screening test for inhibitory effects of dye-stuffs on aerobic waste-water bacteria, later used as the methodical basis of the OECD 209 activated sludge respriation inhibition test, the toxicity of the submission substance to aerobic waste water bacteria had been tested. The EC50 (3 hours) had been determined as > 100 mg/L (defined as at least 80% respiration rate of the control) . This is probably a nominal concentration, however no specific indication is given. The sludge used by the different laboratories was predominantly domestic (4 laboratories used domestic, one of five laboratories however used industrial sludge). It is not clear, which type of sludge had been used with the submission substance. No information on substance purity is given.
However, as this interlaboratory study formed the basis for OECD guideline 209, the data are judged to be reliable (reliability category 2). From these data, the submission substance would be regarded as not harmful to aerobic microorganisms of sewage treatment plants. - Executive summary:
In a publication regarding the development of a screening test for inhibitory effects of dye-stuffs on aerobic waste-water bacteria, later used as the methodical basis of the OECD 209 activated sludge respriation inhibition test, the toxicity of the submission substance to aerobic waste water bacteria had been tested. The EC50 (3 hours) had been determined as > 100 mg/L (defined as at least 80% respiration rate of the control) . This is probably a nominal concentration, however no specific indication is given. The sludge used by the different laboratories was predominantly domestic (4 laboratories used domestic, one of five laboratories however used industrial sludge). It is not clear, which type of sludge had been used with the submission substance. No information on substance purity is given.
However, as this interlaboratory study formed the basis for OECD guideline 209, the data are judged to be reliable (reliability category 2). From these data, the submission substance would be regarded as not harmful to aerobic microorganisms of sewage treatment plants.
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- .see Rationale and Justification for the Analogue Read-Across Approach used for the Registration of Pigment Yellow 14 (Chapter 13)
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 3 h
- Dose descriptor:
- IC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The respiration rate for the submission substance at a concentration of 100 mg/L was >= 80% of the control samples without test item.
- Results with reference substance (positive control):
- Concentration of 3,5-dichlorophenol [mg/L] :: mean inhibition [%]:
100 :: 90%
50 :: 91%
25 :: 78%
10 :: 36%
1 :: 6% - Validity criteria fulfilled:
- not specified
- Remarks:
- However, as validity criteria for guideline OECD 209 were specified, these criteria were probably fulfilled for the reported test results.
- Conclusions:
- In a publication regarding the development of a screening test for inhibitory effects of dye-stuffs on aerobic waste-water bacteria, later used as the methodical basis of the OECD 209 activated sludge respriation inhibition test, the toxicity of the submission substance to aerobic waste water bacteria had been tested. The EC50 (3 hours) had been determined as > 100 mg/L (defined as at least 80% respiration rate of the control) . This is probably a nominal concentration, however no specific indication is given. The sludge used by the different laboratories was predominantly domestic (4 laboratories used domestic, one of five laboratories however used industrial sludge). It is not clear, which type of sludge had been used with the submission substance. No information on substance purity is given.
However, as this interlaboratory study formed the basis for OECD guideline 209, the data are judged to be reliable (reliability category 2). From these data, the submission substance would be regarded as not harmful to aerobic microorganisms of sewage treatment plants. - Executive summary:
In a publication regarding the development of a screening test for inhibitory effects of dye-stuffs on aerobic waste-water bacteria, later used as the methodical basis of the OECD 209 activated sludge respriation inhibition test, the toxicity of the submission substance to aerobic waste water bacteria had been tested. The EC50 (3 hours) had been determined as > 100 mg/L (defined as at least 80% respiration rate of the control) . This is probably a nominal concentration, however no specific indication is given. The sludge used by the different laboratories was predominantly domestic (4 laboratories used domestic, one of five laboratories however used industrial sludge). It is not clear, which type of sludge had been used with the submission substance. No information on substance purity is given.
However, as this interlaboratory study formed the basis for OECD guideline 209, the data are judged to be reliable (reliability category 2). From these data, the submission substance would be regarded as not harmful to aerobic microorganisms of sewage treatment plants.
Referenceopen allclose all
Description of key information
To assess the possible impact of the Diarylide Yellow Pigments on sewage treatment plant microbial activity, a reliable Activated Sludge Respiration Inhibition Test for Pigment Yellow 13 is available. At a pigment concentration far above the solubility limit, no respiration inhibition was observed (≥ 80% of the control). This is supported by a non-guideline study which focused on possible long term microbial degradation of Pigment Yellow 13. By light microscopy different microorganisms growing on the particle surface could be detected, arguing against microbial toxicity of the pigment. Judging from these experimental results, Diarylide Yellow Pigments are nontoxic to sewage treatment plant microorganisms.
Key value for chemical safety assessment
Additional information
To assess the possible impact of substances of the Pigment Yellow 12 and its strucutral analogues, the Diarylide Yellow Pigments, on sewage treatment plant microbial activity, a reliable Activated Sludge Respiration Inhibition Test for Pigment Yellow 13 is available (reliability category 2). At a pigment concentration far above the solubility limit, no respiration inhibition was observed (≥ 80% of the control). This is supported by a non-guideline study which focussed on possible long term microbial degradation of Pigment Yellow 13. By light microscopy different microorganisms growing on the particle surface could be detected, arguing against microbial toxicity of the pigment.
Judging from these experimental results, Diarylide Yellow Pigments are nontoxic to sewage treatment plant microorganisms.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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