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Registration Dossier
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EC number: 249-854-8 | CAS number: 29797-40-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Biodegradation in soil
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in soil, other
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1993
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- The substance described in this dossier is an isomeric mixture of five position isomers of dichlorotoluene. The similarities concerning chemical structure and key intrinsic properties such as log Kow and water solubility lead to the assumption that the other intrinsic values and values for ecotoxicological endpoints will also be very similar for the individual isomers and the mixture of isomers and that therefore results obtained with isomers can be useful for the assessment of the mixture.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The anaerobic metabolism was evaluated in soil slurry microcosms under anaerobic, methanogenic conditions. The substances and their metabolites were extracted with pentane and analysed by GC.
- GLP compliance:
- not specified
- Test type:
- laboratory
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not reported - Radiolabelling:
- no
- Oxygen conditions:
- anaerobic
- Soil classification:
- not specified
- Soil no.:
- #1
- Soil type:
- clay loam
- % Org. C:
- 5.38
- pH:
- 7.2
- Details on soil characteristics:
- The used soil contained 37.0 ppm nitrogen and 16.5 ppm phosphorus . The pH was 7.2, the organic matter content was 5.38%.
The soil was air dried, passed through a 3.25-mm-pore-diameter sieve, and immediately transferred to an anaerobic glove box before use. - Soil No.:
- #1
- Duration:
- 150 d
- Soil No.:
- #1
- Initial conc.:
- 800 other: µM 2,4 DCT, corresponding to 129 mg/L
- Soil No.:
- #1
- Initial conc.:
- 1 000 other: µM 2,5 - DCT, corresponding to 161 mg/L
- Soil No.:
- #1
- Initial conc.:
- 1 000 other: µM 3,4 - DCT; corresponding to 161 mg/L
- Soil No.:
- #1
- Initial conc.:
- 1 000 other: µM 2,6 - DCT, corresponding to 161 mg/L
- Soil No.:
- #1
- Initial conc.:
- 750 other: µM 2,3 - DCT, corresponding to 121 mg/L
- Parameter followed for biodegradation estimation:
- test mat. analysis
- other: monitoring of primary degradation products
- Soil No.:
- #1
- Temp.:
- not reported
- Humidity:
- not reported
- Microbial biomass:
- 10% soil microbial culture
- Details on experimental conditions:
- Preparation of soil slurries: 50 g soil and 70 mL of sterile anaerobic mineral medium were placed into 150-mL serum bottles. All bottles received 1 mM sodium sulfitde and resazurin as an anaerobic indicator. The bottles were sealed with Teflon-lined butyl rubber stoppers and crimped. A H2-to CO2 head-space ratio of 80:20 was set for the methanogenic conditions. The DCT-mixture was amended and inoculated with a 10% slurry microbial culture.
To determine the degradation capabilities of the endogeneous soil microbiota, one set of soil slurry was not inoculated. Further, one set of poisoned controls was run with 15 mM sodium azide (Na N3). - Soil No.:
- #1
- % Degr.:
- ca. 100
- Parameter:
- other: test material analysis of 2,4-DCT, 2,5-DCT and 3,4-DCT
- Sampling time:
- 130 d
- Soil No.:
- #1
- DT50:
- ca. 90 d
- Remarks on result:
- other: 2,4-DCT; 2,5-DCT and 3,4-DCT
- Transformation products:
- yes
- No.:
- #1
- No.:
- #2
- No.:
- #3
- Executive summary:
The anaerobic metabolism of a mixture of isomers was evaluated in soil slurry microcosms under anaerobic, methanogenic conditions. The substances and their metabolites were extracted with pentane and analysed by GC. Results:
- The DT50 for microbial transformation of 2,4 -DCT, 2,5 -DCT and 3,4-DCT was determined to be ca. 90 d under the test conditions.
Transformation products were 4 -, 3 - and 2-chlorotoluene as well as toluene.
- Slower degradation was found for 2,3 -DCT and 2,6-DCT (according to the authors "not susceptible to the microorganisms"). Reading from the graphs 2,3 -DCT declined from 780 to 500 µmole and 2,6 -DCT from 1000 to 600 µmole within 120 to 150 days.
The concentrations at which the DCT isomers were tested (121, 129 or 161 mg/L) are about 10 times higher than the LC50 determined for 3,4 -DCT in the "toxicity to microorganisms test" by Schultz, 1999. Therefore, it might be assumed that the test concentrations negatively influenced the metabolisation of the DCT isomers in soil slurry.
Reference: Ramanand, 1993
Reference
Biotransformation of 2,4-DCT and 3,4-DCT resulted predominantly in the formation of 4-Chlorotoluene (4 -CT) and subsequently of toluene.
2,5-DCT was transformed to toluene via 2-CT with small amounts of 3-CT.
2,3-DCT and 2,6-DCT were found to be not susceptible to the microorganisms.
The initial concentrations and the DT50 - and DT 100 values were determinated visually from the graphs provided in the publication for the citation in HSDB.
Description of key information
A microcosms study in soil slurry under anaerobic, methanogenic test conditions (Reference: Ramanand, 1993) provided the following results:
- DT50 of ca. 90 days for the three main isomers 2,4-DCT, 2,5-DCT and 3,4-DCT. Transformation products were 4-CT, 3-CT and 2-CT as well as toluene.
- Slower degradation was found for 2,3-and 2,6-DCT (according to study author “not susceptible to microorganisms”).
The concentrations at which the DCT isomers were tested (121, 129 or 161 mg/L) are about 10 times higher than the LC50 determined for 3,4 -DCT in the "toxicity to microorganisms test" by Schultz, 1999. Therefore, it must be assumed that the test concentrations negatively influenced the metabolisation of the DCT isomers in soil slurry.
Key value for chemical safety assessment
- Half-life in soil:
- 90 d
Additional information
The anaerobic metabolism of individual isomers was evaluated in soil slurry microcosms under methanogenic conditions. The substances and their metabolites were extracted with pentane and analysed by GC. Results:
- The DT50 for microbial transformation of 2,4-DCT, 2,5-DCT and 3,4-DCT was determined to be ca. 90 d under the test conditions. Transformation products were 4 - , 3 - and 2 - chlorotoluene as well as toluene.
- Slower degradation was found for 2,3 -DCT and 2,6-DCT (according to the authors "not susceptible to the microorganisms"). Reading from the graphs, 2,3 -DCT and 2,6 -DCT declined by about 40% within 120 to 150 days.
The concentrations at which the DCT isomers were tested (121, 129 or 161 mg/L) are about 10 times higher than the LC50 determined for 3,4 -DCT in the "toxicity to microorganisms test" by Schultz, 1999. Therefore, it might be assumed that the test concentrations negatively influenced the metabolisation of the DCT isomers in soil slurry.
Based on this result the substance is not persistent and not highly persistent (half life in soil >180 days) according to REACH guidance document Table R. 7.11-2 (ECHA 2012).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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