Diisodecyl adipate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 May - 12 Jun 1996

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Qualifier:

according to guideline

Guideline:

other: OECD 202, part 2 adopted in 1984

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 2 mg/L treatment
- Sampling method: A separate test concentration was prepared and used for testing the stability of the test substance in water.

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution was prepared by by mixing 1 g test substance with 1 g vehicle in 1 L of dionized water. The stock solution was diluted to the respective test concentrations.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): MARLOWET R 40
- Concentration of vehicle in test medium (stock solution and final test solution(s) including control(s)): 2 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): not stated

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Strain/clone: clone 5
- Source: own breeding
- Age of parental stock (mean and range, SD): < 24 hours
- Feeding during test
- Food type: green algae
- Amount: 0.5E7 cells/daphnid from day 0 to day 6; 1.0E7 cells/daphnid from day 7 to day 21
- Frequency: daily

HOUSING
- Housing medium: M4-medium (Elendt, 1990)
- Housing conditions: each test organism were kept separate in 60 mL test water

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test temperature:

20 ± 1 °C

pH:

7.8 - 8.6

Dissolved oxygen:

94 - 107 mg/L

Nominal and measured concentrations:

Nominal test concentrations: 0.5, 1.0, 2.0 mg/L
Measured concentrations: 0.19, 0.39, 0.77 mg/L (geometric mean, biological active)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, total volume, fill volume: glass beakers, 150 mL, 100 mL
- Renewal rate of test solution (frequency): after 2, 6, 7, 9, 12, 14, 16, 19 days
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- No. of vessels per vehicle control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: synthetic freshwater according to Elendt (M4-medium)
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH-value and oxygen content of the new test solutions at day 2, 12, 16 and of the old test solutions at day 6, 14, 19.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours darkness
- Light intensity: approx. 450 and 95 Lux

EFFECT PARAMETERS MEASURED: Immobility and reproduction was recorded after 2, 6, 7 9, 12, 14, 16, 19, 21 days.

VEHICLE CONTROL PERFORMED: yes

Reference substance (positive control):

no

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 0.77 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

other: reproduction and survival

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 0.77 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

other: reproduction and survival

Details on results:

No significant effects were observed in up to the highest concentration tested.

Table 1: Results of test substance analytics

Nominal concentration [mg/L]	Determination	Fresh test solution	Old test solution (2 days)	geometric mean [mg/L]
2.0	1	1.8	< 0.05	-
	2	1.8	0.86	-
	3	1.9	0.12	-
	mean	1.8	0.33	0.77

Validity criteria fulfilled:

yes

Conclusions:

A 21d long-term toxicity test with Daphnia magna revealed that DEHA does not cause adverse effects to reproduction and survival up to the saturation limit in water.

Executive summary:

The GLP study was performed according to OECD guideline 202, part 2 using Daphnia magna as test organism. Test concentrations from 0.5 to 2 mg/L were tested using Marlowet R40 as vehicle (2 mg/L). The stability of the highest test concentration (2 mg/L) was analytically examined. A geometric mean value of 0.77 mg/L was measured. No significant effects on reproduction and survival were observed up to the highest concentration tested. Thus, the 21d-NOEC value is determined to be > 0.77 mg/L (measured concentration) and > the solubility in water, respectively.

Reference 2

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Justification for type of information:

Please see Section 13 below.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

>= 0.77 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

other: reproduction and survival

Key result

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 0.77 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

other: reproduction and survival

Description of key information

No relevant studies are available for diisodecyl adipate.

A long-term toxicity study in Daphnia Magna is available for the closely related, read-across substance, bis(2-ethylhexyl) adipate (CAS 103-23-1). The GLP study was performed according to OECD guideline 202, part 2 using Daphnia magna as test organism. Test concentrations from 0.5 to 2 mg/L were tested using Marlowet R40 as vehicle (2 mg/L). The stability of the highest test concentration (2 mg/L) was analytically examined. A geometric mean value of 0.77 mg/L was measured. No significant effects on reproduction and survival were observed up to the highest concentration tested. Thus, the 21d-NOEC value is determined to be > 0.77 mg/L (measured concentration) and > the solubility in water, respectively.