REAKTIV-GELB F-68 072 FW

Adsorption / desorption

Link to relevant study record(s)

Reference

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 March 2006 to 22 March 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EU Method C.19 (Estimation of the Adsorption Coefficient (KOC) on Soil and Sewage Sludge Using High Performance Liquid Chromatography (HPLC))

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Radiolabelling:

no

Test temperature:

25 deg C

Details on study design: HPLC method:

Reagents
Methanol, Riedel de Haen
Potassium dihydrogen phosphate, Fluka
Cleaned water (laboratory equipment)
Distilled water, Roth
Dead time marker: Formamide, Merck
Calibration substances (Merck, Riedel de Haen)

Instruments
Chromatographic apparatus
Analytical balance, Sartorius, model R160 P
pH-meter, Mettler, model MP220
Variable pipettes, Brand
General laboratory glassware
Cleaned water plant, Elga

Chromatographic Conditions
Except for the detection wavelength the chromatographic conditions were the same for the determination of t0 the establishment of the calibration graph and the measurement of the test item.
HPLC Equipment: HPLC pump G1312A, autosampler G1313A, column oven G1316A, degaser G1322A and UVA/is detector G1315B by Agilent
Column: stationary phase: LiChrospher 100 CN 5um by Duratec (18704-02) material: stainless steel, dimensions: 125 mm x Ø 4.6 mm column temperature: 25 °C
Mobile Phase: 900 ml cleaned water + 100 ml KH2PO4 (c = 0.02 mol/l) / 900 ml methanol + 100 ml KH2PO4 (c = 0.02 mol/l) (70:30 % (v/v)), pH 8.1, isocratic elution
Flow rate: 1.0 ml/min
Injection volume: 10 μl
Detection wavelength: 210 nm (dead time marker, calibration substances)
270 nm (test item)
Injection amounts: Dead time marker: 22.6 μg
Calibration substances: 0.68 - 1.90 μg
Test item: 0.25 ug
The dead time marker was dissolved in mathanol and diluted 0.1 to 1 in the mobile phase and injected as single standards twice. The calibration substances were dissolved in methanol and diluted 0.1 to 1 in the mobile phase and injected as single standards twice. The item was dissolved in distilled water and diluted 0.1 to 1 in mobile phase and injected as single sample twice.

Analytical monitoring:

not specified

Details on sampling:

Not applicable

Details on matrix:

Not applicable

Details on test conditions:

Not applicable

Computational methods:

Not applicable

Type:

Koc

Value:

< 1.3

Temp.:

25 °C

Remarks on result:

other: Org. carbon data not referenced in report

Details on results (HPLC method):

Table 3: log Koc of the Test Item – 1. Measurement
Test item 1. Inj. tR in min k Log k Log Koc
Reaktiv Gelb F6872 FW 0.826 -0.54 <-0.23 <1.32

Table 5: log Koc of the Test Item – 2. Measurement
Test Item 2. Inj. tR in min k Log k Log Koc
Reaktiv Gelb F6872 FW 0.825 -0.54 <0.20 <1.32

Table 6: log Koc of the Test Item
Log Koc
1. Measurement <1.32
2. Measurement <1.32
Mean <1.32

Tables 1, 2 & 4 attached under any further information.

Adsorption and desorption constants:

Not applicable

Details on results (Batch equilibrium method):

Not applicable

Statistics:

Not applicable

Table 1: Determination of the Dead Time t₀

Dead time marker	tRin min 1. Measurement	tRin min 2. Measurement	tRin min Mean
Formamide	1.790	1.789	1.790

 

Dead time: t₀= 1.790 min

 

Table 2: Calibration Data – 1. Measurement

Calibration Substance	1. Inj. tRin min	k	Log k	Log Koc*
Acetanilide	3.054	0.71	-0.15	1.25
Phenole	2.846	0.59	-0.23	1.32
Atrazin	4.545	1.54	0.19	1.81
Monuron	4.590	1.56	0.19	1.99
Triadimenol	8.837	3.94	0.60	2.40
Linuron	9.767	4.46	0.65	2.59
Naphthalene	10.780	5.02	0.70	2.75

(*from OECD guideline 121)

Regression: logK_oc= a + b x log k

Parameters: a= 1.59

                    b= 1.54

                    r²= 0.9760

Table 4: Calibration Data – 2. Measurement

Calibration substance	2. Inj. tRin min	k	Log k	Log Koc*
Acetanilide	3.072	0.72	-0.14	1.25
Phenole	2.856	0.60	-0.20	1.32
Atrazin	4.465	1.55	0.19	1.81
Monuron	4.597	1.57	0.20	1.99
Triadimenol	8.833	3.94	0.60	2.40
Linuron	9.754	4.45	0.65	2.59
Naphthalene	10.753	5.01	0.70	2.75

(*from OECD guideline 121)

Regression: logK_oc= a + b x log k

Parameters: a= 1.58

                    b= 1.55

                    r²=0.9754

Validity criteria fulfilled:

yes

Conclusions:

The adsorption coefficient of the test item was found to be:

log Koc <1.3

Executive summary:

The objective of this study was the determination of the adsorption coefficient K₀c of the test item according to the consolidated version of Guideline 67/548/EEC Annex V,Part C.19.: estimation of the adsorption coefficient on soil and on sewage sludge using HPLC and OECD test guidelines 121: estimation of the adsorption coefficient on soil and on sewage sludge using HPLC. Study conducted in compliance with GLP.

 

The adsorption coefficient of the test item was determined according to the "HPLC- method".

 

The adsorption coefficient of the test item was found to be:

 

logKoc <1.3

 

The substance is not considered to have the potential to adsorb to soil / sediment within the environment.

Description of key information

The adsorption coefficient of the test item was found to be: log Koc <1.3

Key value for chemical safety assessment

Other adsorption coefficients

Type:

other: Koc

Value in L/kg:

19.953

at the temperature of:

25 °C

Additional information

The objective of this study was the determination of the adsorption coefficient K₀c of the test item according to the consolidated version of Guideline 67/548/EEC Annex V,Part C.19.: estimation of the adsorption coefficient on soil and on sewage sludge using HPLC and OECD test guidelines 121: estimation of the adsorption coefficient on soil and on sewage sludge using HPLC. Study conducted in compliance with GLP.