Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 600-299-3 | CAS number: 102322-83-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 Jul - 31 Jul 2013
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- Qualifier:
- according to guideline
- Guideline:
- other: EN ISO 14593 (1999): Water quality - Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium - Method by analysis of inorganic carbon in sealed vessels (CO2 headspace test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Staatliches Gewerbeaufsichtsamt Hildesheim, Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): activated sludge from a municipal sewage treatment plant (Hildesheim, Germany)
- Laboratory culture: no
- Pretreatment: washed twice with chlorine-free tap water; sludge was allowed to settle and resuspended in mineral salts medium and maintained under aerobic conditions for 2.5 h by aeration; sludge was homogenized with a blender; after sedimentation the supernatant was decanted and maintained in an aerobic condition by aeration with CO2-free air for 5 d.
- Colony-forming units (CFU): 10E+05 - 10E+08 CFU/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 21.21 mg/L
- Based on:
- test mat.
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Additional substrate: no
- Test temperature: 20 ± 1 °C (up tp 26 °C on day 25)
- pH adjusted: no
- Continuous darkness: low light conditions
TEST SYSTEM
- Culturing apparatus: headspace flasks, 120 mL (fill volume: 80 mL; headspace to liquid ratio: 1:2)
- Number of culture flasks/concentration: 3 (5 repliactes at the end)
- Method used to create aerobic conditions: constantly shaken (150 - 200 rpm)
- Measuring equipment: CO2 was determined by IC analysis with a carbon analyser according to DIN EN 1484.
- Test performed in closed vessels due to significant volatility of test substance: yes
- Other: The inoculated test medium was prepared in a test flask (80 mL). 2 µL of the test substance were added to the test item flasks (+ toxicity control) by pipetting them into a glass ring and giving it into the test vessels. Sodium hydroxide solution (0.74 mL 7 mol/L solution to 80 mL medium) was injected to each vessel sampled. The vessels were shaken on an overhead shaker for 1 h at test temperature. Suitable samples were analysed.
SAMPLING
- Sampling frequency: Analysis was carried out at test start, twice in the first two weeks and thereafter every 7 d.
- Sample storage before analysis: analysed directly
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, three bottles
- Abiotic sterile control: no
- Toxicity control: yes, three bottles
- Other: reference susbtance: yes, three bottles - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 76
- Sampling time:
- 28 d
- Remarks on result:
- other: 95% CI: 75 - 77%
- Details on results:
- Within the 10-day time window the 60% level was passed. Therefore, the substance is regarded as readily biodegradable according to the OECD criteria.
The toxicity control attained 65% after 14 d and 87% after 28 d. Thus, the test substance is not considered to be inhibitory to the inoculum. - Results with reference substance:
- The reference substance was degraded to 92% after 14 d and 94% after 28 d (95% CI: 92 - 96%), 60% pass level was reached.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance is readily biodegradable according to the OECD criteria (76%, CO2 evolution after 28 d, meeting the 10-day window; OECD 310).
Reference
Table 1: CO2 production and biodegradation of the test item
|
CO2 production in mg C/L |
|
|
|||||
Day |
Replicate 1 |
Replicate 2 |
Replicate 3 |
Replicate 5 |
Replicate 5 |
MP |
Net MP |
Degr. [%] |
0 |
2.46 |
2.47 |
2.46 |
|
|
0.46 |
- |
- |
1 |
1.43 |
1.40 |
1.47 |
|
|
0.55 |
0.00 |
0 |
3 |
2.03 |
2.11 |
1.93 |
|
|
0.87 |
0.00 |
0 |
7 |
2.18 |
2.06 |
2.01 |
|
|
0.98 |
0.00 |
0 |
10 |
5.85 |
2.42 |
3.02 |
|
|
2.38 |
0.86 |
5 |
14 |
10.59 |
11.28 |
10.55 |
|
|
9.71 |
8.61 |
54 |
21 |
12.59 |
12.06 |
12.90 |
|
|
11.49 |
10.38 |
65 |
28 |
14.29 |
14.62 |
14.20 |
14.30 |
14.50 |
13.57 |
12.02 |
76 |
MP: mean values (NaOH corrected)
Net MP: netto mean production (corrected for inoculum control values)
Description of key information
One experimental study is available investigating the ready biodegradability of the test substance. The study was performed according to the OECD 310 (GLP). Activated sludge from a municipal sewage treatment plant was used as inoculum. 76% degradation of the test substance was recorded based on CO2 evolution after 28 d. Since the 10-day window was met the test substance is considered to be readily biodegradable. The toxicity control (containing both, reference substance and test substance) attained 65% and 87% degradation after 14 and 28 d, respectively. Thus, the test substance is not considered to be inhibitory to the inoculum.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.