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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_environmental-fate-and-pathways.png)
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2011-12-21 to 2012-03-28
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Testing Methods for New Chemical Substances; No. 0331-7, Ministry of Health, Labour and Welfare; 2011.03.29, No. 5, Ministry of Economy, Trade and Industry; No. 110331009, Ministry of Environment, Japan.
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Details on test material:
- - Name of test material (as cited in study report): PSF-58
- Physical state: white crystalline powder
- Analytical purity: 100%
- Lot/batch No.: KNC110310
- Stability under test conditions: stable for 2 years in ambient temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge: standard activated sludge purchased from Chemicals Evaluation and Research Institute, Japan
- Laboratory culture: cultured at the test facility in 25 °C (± 2 °C), not less than 5 mg/L dissolved oxygen and pH 7 (± 1).
- Concentration of sludge: 2979 mg/L - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimationopen allclose all
- Parameter followed for biodegradation estimation:
- O2 consumption
- Parameter followed for biodegradation estimation:
- test mat. analysis
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Test temperature: 25.0 °C
- pH: 6.78 - 6.92 (sludge + test substance), 5.92 (water + test substance)
- pH adjusted: yes
- Suspended solids concentration: 30 mg/L
TEST SYSTEM
- Culturing apparatus: test flasks with a fill volume of 300 mL
- Number of culture flasks/concentration: 3
- Measuring equipment: Coulometer Type OM-2001A, Ohkura Electric Co., Ltd
SAMPLING
- Sampling frequency: (BOD) continuous
- Sampling method: (BOD) manometric method equipped with Coulometer
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 1 replicate
- Abiotic sterile control: no
- Toxicity control: no
- Other: Abiotic control: yes, 1 replicate; Activity control: yes, 1 replicate
Reference substance
- Reference substance:
- aniline
Results and discussion
- Test performance:
- This test is valid because the differences between the maximum and the minimum in the degradation rates of " sludge + test substance " were less than 20% ( 5% from BOD, 4% from direct determination ( LC ) and 6% from TOC ), and the degradation rate of activity control substance, aniline, calculated from BOD was not less than 60% ( 83% ) after 14 days exposure.
% Degradationopen allclose all
- Parameter:
- % degradation (O2 consumption)
- Value:
- 1
- Sampling time:
- 28 d
- Remarks on result:
- other: BOD, mean of three replicates
- Parameter:
- % degradation (test mat. analysis)
- Value:
- 19
- Sampling time:
- 28 d
- Remarks on result:
- other: LC-analysis, mean of three replicates
- Parameter:
- % degradation (TOC removal)
- Value:
- 11
- Sampling time:
- 28 d
- Remarks on result:
- other: TOC analysis, mean of three replicates
BOD5 / COD results
- Results with reference substance:
- 83% after 14 d, 60% pass level was reached
Any other information on results incl. tables
Table 1: Degradation rates based on oxygen consumption
|
|
|
7th day |
14th day |
21st day |
28th day |
||||||||
Test solutions |
Amount of test substance added [mg] |
TOD [mg] |
BOD [mg] |
Degradation rate |
BOD [mg] |
Degradation rate |
BOD [mg] |
Degradation rate |
BOD [mg] |
Degradation rate |
||||
Sludge + aniline |
29.9 |
89.9 |
69.6 |
73 |
80.4 |
83 |
83.0 |
84 |
86.3 |
86 |
||||
Control blank |
- |
- |
4.2 |
- |
6.1 |
- |
7.6 |
- |
9.1 |
- |
||||
Sludge + test substance |
30.0 |
37.8 |
4.2 |
0 |
3 |
7.5 |
4 |
7 |
8.7 |
3 |
5 |
8.7 |
-1 |
1 |
Sludge + test substance |
30.0 |
37.8 |
5.0 |
2 |
8.2 |
6 |
9.6 |
5 |
9.6 |
1 |
||||
Sludge + test substance |
30.0 |
37.8 |
6.5 |
6 |
9.7 |
10 |
10.5 |
8 |
10.5 |
4 |
||||
Water + test substance |
30.0 |
- |
0.0 |
- |
0.1 |
- |
0.1 |
- |
1.2 |
- |
Table 2: Material balance by direct determination (LC-analysis)
Test solutions |
Residual rate of test substance [%] |
Production rate of transformation product 1 [%] |
Production of transformation product 2 [%] |
Material balance [%] |
|
Sludge + test substance |
83 |
9 |
0 |
92 |
94 |
Sludge + test substance |
86 |
11 |
0 |
97 |
|
Sludge + test substance |
82 |
10 |
0 |
92 |
|
Water + test substance |
104 |
0 |
0 |
104 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test substance was not readily biodegradable in the test.
- Executive summary:
The biodegradation test was conducted in accordance with OECD 301C.
From results of oxygen consumption (BOD) measurement, the degradation rate was 1 % on average.
From results by LC analysis, the residual rate of test item was 84% on average in "sludge + test substance" and 104% in "water + test substance ", and the degradation rate of test item was 19% on average.
From results by IC analysis, the production rate of sulphate ion was 15% on average in "sludge + test substance" and 0%"' in "water + test substance ".
From results by total organic carbon (TOC) measurement, the residual rate was 98% on average in "sludge + test substance " and 110% in " water + test substance ", and the degradation rate was 11 % on average.
It is concluded from these results that the test substance was not readily biodegradable in the test.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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