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EC number: 219-203-2 | CAS number: 2386-57-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
The mutagenic potential of sodium methanesulfonate was evaluated using studies performed with the methanesulfonic acid. In the condition of the in vitro assay, methanesulfonic acid and the sodium salt are equivalent, considering that the the acid was tested up to the limit dose recommended by the test guidelines.
In the key study, the genotoxic potential of Methanesulfonic acid was assessed by the Ames test on five Salmonella typhimurium tester strains: TA1535, TA1537, TA1538, TA100 and TA98, both in the absence and presence of metabolic activation (Dechariaux, 1990). Methanesulfonic acid was dissolved in distilled water and tested at concentrations ranging from 100 to 10000 µg/plate. The compound proved to be very toxic (inhibition of bacterial background lawn and diminution of the reverse colonies number) on TA100 and TA98, with and without metabolic activation at 10000 µg/plate only. Without metabolic activation at 5000 µg/plate, the toxic effect observed was less pronounced on TA 100, and TA98; and for TA98 only, the colonies revertant number diminished without inhibition of the background lawn. Consequently, the concentrations selected for the genotoxicity studies were 100, 500, 1000, 2500 and 5000 µg/plate. Whether in the presence or in the absence of metabolic activation, no increase was observed in the number of His+ revertant colonies/plate at any of the concentrations tested, on the five Salmonella typhimurium tester strains, in either study. An inhibition of bacterial background lawn was also noted for the five strains at 5000 µg/plate with metabolic activation. The diminution of the number of the reverse colonies at 5000 µg/plate for some strains was not reproducible in the two studies, which seemed to indicate that this concentration is at the lirnit of methanesulfonic acid toxicity, with metabolic activation. In addition, at 5000 µg/plate the compound provoked a precipitate of the S-9 mix preparation. In conclusion, Methanesulfonic acid was not genotoxic in the Ames test, with and without mctabolic activation.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- 1983 version of guideline
- Deviations:
- yes
- Remarks:
- Contradictory information concerning precipitation
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
- Species / strain / cell type:
- S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-treated rat liver S9-mix
- Test concentrations with justification for top dose:
- 100, 500, 1000, 2500 and 5000 µg/plate
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: distilled water
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- distilled water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- 2-nitrofluorene
- sodium azide
- other: 2-aminoanthracene
- Remarks:
- TA1535 and TA100: 5 µg/plate
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
DURATION
- Preincubation period: 20 minutes
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS: 3/dose
DETERMINATION OF CYTOTOXICITY
- Method: other: decrease of revertant colonies - Evaluation criteria:
- Criteria for cytotoxicity:
Reduced numbers of revertant colonies/plate compared with control plates, sparsity of background lawn when compared with control plates.
Criteria for genotoxocity:
Number of revertants at least twice that of spontaneous revertants, dose-related pattern, number of revertants/nmol > 0.01, reproducibility of the positive resonse. - Statistics:
- No data on statistical test used.
- Key result
- Species / strain:
- S. typhimurium, other: TA 1535, TA 1537, TA 1538, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- 5000 µg/plate
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: A precipitation of the S9-mix preparation was observed from the concentration of 2500 µg/plate in a concentration-related effect of increasing intensity in the first assay, while it only appeared at 5000 µg/plate in the second one.
BACTERIAL TOXICITY:
Methane sulfonic acid induced a marked toxicity on two tester strains: TA98 and TA100 at 1000 µg/plate with and without metabolic activation. In addition, it was observed that the S9-mix precipitated at those two concentrations.
Without metabolic activation at 5000 µg/plate, a moderate toxic effect was noted on TA100, whereas on TA98 a decrease of the revertant colony numbers with a sparse background was noted.
Therefore, the currently used concentrations were selected for the genotoxicity study.
First study (CEL473A)
With or without a metabolic activation system, Methanesulfonic acid did not inducc any increase of the number of His+ revertant colonies/plate, whatever the concentration. With metabolic activation, an inhibition of the bacterial background lawn was observed on the five tester strains at 5000 µg/plate, associated to a diminution of the revertant colonies number for TA1535 and TA1538 only. No such diminution was notcd on the other tester strains: therefore, the toxic effect noted on TA98 and TA100 was not as marked as that observed during the preliminary study. Furthermore, precipitation of the S-9 mix preparation was observed from the concentration of 2500 µg/plate, in a concentration-related effect of increasing intensity.
Second study (CEL473B)
There was no increase in the number of His+ revertant colonies/plate at either dose of Methanesulfonic acid on any of the five Salmonella typhimurium tester strains.
The results of this second study confirm the non-genotoxicity of Methanesulfonic acid at the highest concentration of 5000 µg/plate and at lower concentrations, both in the presence and absence of metabolic activation.
During the second study, without metabolic activation, an inhibition of the bacterial background lawn was observed on the five tester strains studied, with diminution of the revertant colonies number on TA1537, TA1538, TA98 and TA100 only.
These observations somewhat differ from those noted during the first study and seem to indicate that the concentration of 5000 -µg/plate, with metabolic activation, is at the limit of toxicity. Furthermore, S-9 mix precipitated at 5000 µg/plate. - Conclusions:
- Methanesulfonic acid was not genotoxic in the Ames test, with and without metabolic activation.
- Executive summary:
The genotoxic potential of Methanesulfonic acid was assessed by the Ames test on five Salmonella typhimurium tester strains: TA1535, TA1537, TA1538, TA100 and TA98, both in the absence and presence of metabolic activation. Methanesulfonic acid was dissolved in distilled water and tested at concentrations ranging from 100 to l0000 µg/plate. The compound proved to be very toxic (inhibition of bacterial background lawn and diminution of the reverse colonies number) on TA100 and TA98, with and without metabolic activation at 10000 µg/plate only. Without metabolic activation at 5000 µg/plate, the toxic effect observed was less pronounced on TA 100, and TA98; and for TA98 only, the colonies revertant number diminished without inhibition of the background lawn. Consequently, the concentrations selected for the genotoxicity studies were 100, 500, 1000, 2500 and 5000 µg/plate. Whether in the presence or in the absence of metabolic activation, no increase was observed in the number of His+ revertant colonies/plate at any of the concentrations tested, on the five Salmonella typhimurium tester strains, in either study. An inhibition of bacterial background lawn was also noted for the five strains at 5000 µg/plate with metabolic activation. The diminution of the number of the reverse colonies at 5000 µg/plate for some strains was not reproducible in the two studies, which seemed to indicate that this concentration is at the lirnit of methanesulfonic acid toxicity, with metabolic activation. In addition, at 5000 µg/plate the compound provoked a precipitate of the S-9 mix preparation. In conclusion, Methanesulfonic acid was not genotoxic in the Ames test, with and without mctabolic activation.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Justification for classification or non-classification
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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