(5or8)-aminonaphthalene-2-sulphonic acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed publication

Data source

Reference

Reference Type:

publication

Title:

Mutagenicity testing of some commonly used dyes.

Author:

K T Chung, G E Fulk and A W Andrews

Year:

1981

Bibliographic source:

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Oct. 1981, p. 641-648

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

Gene mutation toxicity study was performed to evaluate the mutagenic response for the test chemical sodium 4-aminonaphthalene-1-sulphonate.

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Sodium 4-aminonaphthalene-1-sulphonate

EC Number:

204-975-5

EC Name:

Sodium 4-aminonaphthalene-1-sulphonate

Cas Number:

130-13-2

IUPAC Name:

sodium 4-aminonaphthalene-1-sulfonate

Details on test material:

CAS number: 130-13-2
Chemical Name: sodium 4-aminonaphthalene-1-sulphonate
Molecular formula: C10H9NO3S.Na
Molecular weight: 245.233 g/mol
Substance type: Organic
Smiles: c12c(c(ccc1S(=O)(=O)[O-])N)cccc2.[Na+]

Method

Target gene:

No data

Species / strain

Species / strain 1

Species / strain / cell type:

S. typhimurium TA 1538

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Species / strain 2

Species / strain / cell type:

S. typhimurium TA 98

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Species / strain 3

Species / strain / cell type:

S. typhimurium TA 100

Details on mammalian cell type (if applicable):

Not applicable

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

rat Aroclor S9 mix

Test concentrations with justification for top dose:

5, 10, 25, 50, 100, 250, 500, 1000, 2500, 5000 µg

Vehicle / solvent:

No data

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

not specified

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

2-nitrofluorene

sodium azide

other: Dimethyl sulfoxide,2-Aminoanthracene

Details on test system and experimental conditions:

The Salmonella typhimurium tester strains TA1535, TA1537, TA1538, TA98,and TA100 were grown in nutrient broth shaken for 14 h at 370C. The plate incorporation assays were performed as described by Ames with the modifications of Andrews. The liquid preincubation assays (1, 21) were timed for 30 min at 37°C in a Dri-block. The revertant colonies were counted by using a hand-held tally. Dimethyl sulfoxide was the solvent, except sterile distilled water was the solvent when sulfanilic acid was used. Liver homogenates (S9) were prepared from male Sprague-Dawley rats stimulated with Aroclor 1254 (500 mg/kg intraperitoneally 5 days before sacrifice). The S9 mix, added in samples of 0.5 ml per plate, contained 3 mg of protein, determined by the method of Lowry et al. (16). The positive control chemicals sodium azide, 9-aminoacridine, 2-nitrofluorene, and 2-aminoanthracene were used with the tester strains. The dose-response curves for the mutagenic compounds used the tester strain which showed maximum mutagenicity and covered a range of 1 to 1,000 or 5 to 5,000 tig. Mutagenic compounds were assayed by using duplicate plates in at least two independent dose-response curves; the mean values of representative curves are used in the table. A compound was considered mutagenic when the number of revertants above background was at least twice the value of the historical control mean or twice the value of the current control mean, whichever was greater,and a dose-response curve could be demonstrated

Evaluation criteria:

An increase in the number of revertnats was counted

Statistics:

No data

Results and discussion

Test results

Test results 1

Species / strain:

S. typhimurium TA 1538

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Test results 3

Species / strain:

S. typhimurium TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Additional information on results:

No data

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative with and without

Maximum nontoxic dose of test substance sodium 4-aminonaphthalene-1-sulphonate was tested that was nonmutagenic in TA1535, TA1537, TA1538, TA98,or TA100 with and without male rat Aroclor S9 mix.

Executive summary:

Gene mutation toxicity study was performed to evaluate the mutagenic response for the test chemical sodium 4-aminonaphthalene-1-sulphonate. The study was performed using Salmonella typhimurium strains TA1537, TA1538, TA98,or TA100 with and without S9 metabolic activation system at dose levels of 5, 10, 25, 50, 100, 250, 500, 1000, 2500, 5000 µg.

Maximum nontoxic dose of test substance sodium 4-aminonaphthalene-1-sulphonate was tested that was nonmutagenic TA1537, TA1538, TA98,or TA100 with and without male rat Aroclor S9 mix. Thus, the test compound is not likely to be gene mutant in vitro.