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EC number: 700-453-0 | CAS number: 59802-05-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2010-01-21 to 2011-03-22
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2011
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- (1984)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,4-Benzenedicarboxylic acid, 1,4-diisononyl ester
- EC Number:
- 700-453-0
- Cas Number:
- 59802-05-0
- Molecular formula:
- C26H42O4
- IUPAC Name:
- 1,4-Benzenedicarboxylic acid, 1,4-diisononyl ester
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- yes
- Remarks:
- acetone
- Details on preparation and application of test substrate:
- - Method of mixing into soil (if used): An aliquot (35 ml) of the 5000 mg/50 mL solvent stock solution was added to approximately 200 g of artificial soil and the solvent allowed to evaporate off prior to incorporation into a final amount of 3.5 kg (dry weight) of artificial soil with 980 ml of deionised reverse osmosis water. This was mixed with a mixer to give the 1000 mg/kg test concentration.
- Controls: The control was prepared in a similar manner, without the addition of solvent or test item. Solvent control (acetone). The control and solvent control soil was dispersed to four replicates and the test soil was dispersed to six replicates.
- Chemical name of vehicle: acetone
- Evaporation of vehicle before use: The solvent was allowed to evaporate off prior to incorporation into artificial soil.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: Eisenia fetida
- Source: Original Organics Limited, Langlands Business Park, Devon, UK
- Weight at test initiation: 0.30 g - 0.48 g
- The test organisms were with clitellum and were transferred into artificial soil approximately 24 hours prior to the start of the test.
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Remarks:
- Industrial quartz sand: 70% w/w Kaolinite clay: 20% w/w Sphagnum moss peat: 10% w/w
- Limit test:
- yes
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- 21°C to 23°C.
- pH:
- 6.0 ± 0.5
- Moisture:
- The moisture content of the soil used in the definitive test was 29% of dry weight of basic substrate on Day 0 and 27% to 28% on Day 14.
- Details on test conditions:
- Light intensity was 423 to 580 lux
- Nominal and measured concentrations:
- Based on the results of the range-finding test a "limit test" was conducted at a concentration of 1000 mg/kg of soil.
- Reference substance (positive control):
- yes
- Remarks:
- chloroacetamide
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- There were no mortalities or sub-lethal effects of exposure in 60 earthworms exposed to a test concentration of 1000 mg/kg for a period of 14 days. It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/kg.
The No Observed Effect Concentration (NOEC) was greater than or equal to 1000 mg/kg on the basis that no mortalities were observed after 14 days exposure and additionally no sub-lethal effects on weight or behaviour were observed at 1000 mg/kg. - Results with reference substance (positive control):
- - Results with reference substance valid: The results were within the normal range for this reference item of 10 to 50 mg/kg.
- Relevant effect levels: 14-d LC50= 32 mg/kg with 95% confidence limits of 29 - 35 mg/kg. NOEC=18 mg/kg - Reported statistics and error estimates:
- Statistical analysis using Bartlett's test for homogeneity of variance (Sokal and Rohlf 1981) and a Students t-test was carried out on the earthworm weight data.
The earthworm weights for the control at Day 0 were found to be significantly heavier (P<0.05) compared to the 1000 mg/kg test group, however as there were no significant differences in terms of weight between the solvent control and 1000 mg/kg test group, and the direct comparison was between the solvent control and 1000 mg/kg test concentration, it was considered that the differences observed on Day 0 did not affect the outcome or validity of the test. Review of the data suggests that this difference was likely due to a small number of particularly heavy individuals in the control group. There were no significant differences (P~0.05) between the solvent control, control and 1000 mg/kg test group in terms of weight on Day 14.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test item, to the earthworm Eisenia foetida, in an artificial soil test has been investigated and gave a 14-Day LC50 value of greater than 1000 mg/kg.
Correspondingly the No Observed Effect Concentration was greater than or equal to 1000 mg/kg.
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