Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 253-197-2 | CAS number: 36768-62-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP study according to guideline
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 009
- Report date:
- 2009
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 2,2,6,6-tetramethyl-4-piperidylamine
- EC Number:
- 253-197-2
- EC Name:
- 2,2,6,6-tetramethyl-4-piperidylamine
- Cas Number:
- 36768-62-4
- Molecular formula:
- C9H20N2
- IUPAC Name:
- 2,2,6,6-tetramethylpiperidin-4-amine
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken after 0h, 24h, 48h and 72h and the concentration of the test item was determined.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The test concentrations were prepared by diluting a stock solution of 1 g/L. 200 mg test substance was weighed into a beaker, mixed with culture medium and was stirred for 5 min. with a magnetic stirrer to dissolve the test substance in the test medium. Then the test substance preparation was filled into a graduated flask and made up to a total volume of 200 mL with culture medium.
The amounts corresponding to the test concentrations were pipetted into a graduated flask, made up to a total volume of 1000 mL with culture medium, and then transferred to the test vessels (for dilution scheme, see table below).
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- The unicellular algae Desmodesmus subspicatus was used as test organism. The organism (Strain 86.81) was obtained from “Sammlung von Algenkulturen”, Göttingen (SAG), (Collection of Algae Cultures, Institute for Plant physiology), University of Göttingen, Germany.
Three days before the start of the main experiment two flasks with 100 ml culture medium were prepared and 5 x 105 algae from stock culture were added, resulting in a final concentration of 5 x 103 cells per mL. After three days of incubation the cell concentration was determined by particle counter and was at least 16 x 5000 cells per mL. The mean of 5 measurements was calculated.
For the test an appropriate volume of the pre-culture with an amount of 7.5*10 000 cells per ml were used.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- no data
- Test temperature:
- 25 +/- 1°C
- pH:
- test start: 8.1 - 9.8
test end: 8.4 -9.4 - Dissolved oxygen:
- no data
- Salinity:
- 0
- Nominal and measured concentrations:
- nominal: 6.25, 12.5, 25.0, 50.0 and 100 mg/L
Recovery of the test substance at test end (48h) was between 99% and 114% of nominal values. - Details on test conditions:
- according to guideline
- Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 82.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC20
- Effect conc.:
- 122 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 255.8 mg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL 229.9-292.3
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 25 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 50 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- ErC50 (72h): 0.8 mg/l
- Reported statistics and error estimates:
- EC50 values, 95% confidence limits and NOEC were calculated with the ToxRat Professional software.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In an algal growth inhibition test using Desmodesmus subspicatus as test species, the test substance showed no effects on growth rate after 72h exposure up to concentrations of 25 mg/l (NOEC). The EC50 is considered to be > 100 mg/L (calculated as 255.8 mg/L) under conditions tested.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)