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EC number: 305-644-9 | CAS number: 94891-43-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 18 Oct - 02 Nov 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 994
- Report date:
- 1994
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- - Principle of test: Determination of toxic effects of the test item to anaerobic bacteria through reduction of gas production compared to untreated control
- Short description of test conditions:
- test item: Remazol-Brillantgrün 6B
- test duration: 7 days
- temperature 37°C +/- 1°C
- concentrations of the test item: 700 mg/L to 1000 mg/L with ech 3 replicates
- reference item: Dodecyldimethylbenzylammoniumchlorid 50, 100, 200 mg/L
- test vessels: 100 mL bottles filled with 30 mL test medium
- Parameters analysed / observed: injection of phosphoric acid (85%) 30 minutes before measuring the CO2 pressure in the headspace of the bottles.. - GLP compliance:
- yes
Test material
- Reference substance name:
- Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts
- EC Number:
- 305-644-9
- EC Name:
- Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts
- Cas Number:
- 94891-43-7
- Molecular formula:
- C32 H16 N8 Ni . C8 H11 N O6 S2 . 2 Na
- IUPAC Name:
- Nickel, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, chlorosulfonyl derivs., reaction products with 2-[(4-aminophenyl)sulfonyl]ethyl hydrogen sulfate monosodium salt, sodium salts
- Test material form:
- liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution: 1000 mg test item to 450 mL, pH adjusted from 4,7 to 7,2, stirred for 1,5 hours at 22°C, afterwards pH adjusted from 6,9 to 7,3, stock solution finally filled to 500 mL (concentration: 2000 mg/L). clear solution without undissolved test item
- Controls: yes: test vessel with inoculum, without test item
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): no vehicle
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): -
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): no undissolved material
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: no
- Name and location of sewage treatment plant where inoculum was collected: anerobic activated sludge from domestic waste water treatment plant Kriftel
- Preparation of inoculum for exposure: after pretreatment, addition of nutrition solution according to ISO 11734
- Pretreatment: 9 days at 35°C without additing of nutrient solution
- Initial biomass concentration: concentration of activated sludge in test vessels: 3,5 g/L (dry weight)
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 9 d
Test conditions
- Test temperature:
- 37°C +/- 1°C
- Nominal and measured concentrations:
- nominal concentrations 700 mg/L and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 mL bottles
- Material, size, headspace, fill volume: fill volume: 30 mL
- No. of vessels per concentration (replicates): 3
- Sludge concentration (weight of dry solids per volume): 3.5 g/L (dry weight) - Reference substance (positive control):
- yes
- Remarks:
- Dodecyldimethylbenzyl ammonium chloride
Results and discussion
Effect concentrations
- Key result
- Duration:
- 7 d
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of anaerobic gas production
Any other information on results incl. tables
concentration [mg/L] | inhibition [%] |
1000 | 10 |
700 | 6 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 7-d EC20 to anaerobic bacteria was determined >1000 mg/L (nominal) based on anaerobic gas production.
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