Reaction mass of dodecyl acrylate and tridecyl acrylate

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Meets National Standard Methods (DIN) with acceptable restrictions Read across was performed with Laurylacrylat 12/14 (mixture of laurylacrylate (CAS No.: 2156-97-0) and tetradecylacrylate (CAS No.: 21643-42-5) . Please refer to IUCLID section 13 for read across justification.

Qualifier:

according to guideline

Guideline:

other: German national standard, DIN 38412 Part 27, draft (ROBRA test)

Deviations:

no

GLP compliance:

no

Analytical monitoring:

no

Details on test solutions:

10000 mg test substance/L (nominal) was prepared by using 100 mg/L Tween 80 as a solubilizer and stirring for a period of 2 hours. The corresponding required test concentrations were prepared by taking appropriate volumes of the stirring stock solution. The emulsion appeared milky white and cloudy. Without stirring, dilution water and test substance separated after 5 min. Therefore aliquots were taken under constant stirring.

Test organisms (species):

Pseudomonas putida

Details on inoculum:

TEST ORGANISN
- Pseudomonas putida
- Strain: DSM 50026
- Age of bacterial culture: 2 hours

Water media type:

freshwater

Test temperature:

25 °C

pH:

7.2 - 7.3

Nominal and measured concentrations:

The test concentrations were: 100, 1000 and 10000 mg/L. Additionally, the highest test concentration (10000 mg/L) was tested in dilution water without glucose. A blank control (containing medium only) and a solvent control (containing 100 mg/L Tween 80) were tested in parallel.

Details on test conditions:

TEST SYSTEM
- Aeration: The test solution was aerated for 30 minutes at 25 °C.
- pH: 7.2 - 7.3
- Temperature: 25 °C
- Photometrical measurement at 436 nm

TEST CONCENTRATIONS. The test concentrations were: 100, 1000 and 10000 mg/L. Additionally, the highest test concentration (10000 mg/L) was tested in dilution water without glucose. A blank control (containing medium only) and a solvent control (containing 100 mg/L Tween 80) were tested in parallel.

Duration:

30 min

Dose descriptor:

EC10

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC0

Effect conc.:

>= 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Duration:

30 min

Dose descriptor:

EC50

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Details on results:

The EC50 value was determined to be greater than 10000 mg/L and also the EC10 was determined to be greater than 10000 mg/L.

Reference 2

Endpoint:

activated sludge respiration inhibition testing

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

other information

Reason / purpose for cross-reference:

read-across source

Description of key information

No activated sludge respiration inhibition study is available on the registered substance (Reaction mass of C12-C13 acrylates, EC Number 911-296-4). However, experimental data are available on an analogue substance (Reaction mass of C12-C14 acrylates, CAS 84238-60-8).

In an experimental study conducted according to DIN 38412, Part 27 (ROBRA test), no significant effects were observed up to the highest tested concentration (10000 mg/L). Since no significant effects were observed, the NOEC is equal to or greater than 10000 mg/L.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:

10 000 mg/L