(epoxyethyl)benzene

Eye irritation

Administrative data

Endpoint:

eye irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: No flaws compromising the reliability were observed when the study was evaluated using the ECVAM "ToxRTool"

Data source

Reference

Reference Type:

publication

Title:

Studies on eye irritation caused by chemicals in rabbits -1. A quantitative structure-activity relationships approach to primary eye irritation of chemicals in rabbits-

Author:

Sugai S et al

Year:

1990

Bibliographic source:

The Journal of Toxicological Science, Vol. 15, 245-262, 1990

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 405 (Acute Eye Irritation / Corrosion)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

(epoxyethyl)benzene

EC Number:

202-476-7

EC Name:

(epoxyethyl)benzene

Cas Number:

96-09-3

Molecular formula:

C8H8O

IUPAC Name:

2-phenyloxirane

Test animals / tissue source

Species:

rabbit

Strain:

other: Japanese white rabbit

Details on test animals or tissues and environmental conditions:

TEST ANIMALS- Source: - Age at study initiation: no data- Weight at study initiation: 2.0-2.2 kg- Housing: metal cages (39x40x44 cm)- Diet (e.g. ad libitum): RC4 diet (Oriental Yeast Co., Tokyo, Japan)- Water (e.g. ad libitum): ad libitum - Acclimation period: no dataENVIRONMENTAL CONDITIONS- Temperature (°C): 25+/- 1°C- Humidity (%): 55 +/-5%- Air changes (per hr): no data- Photoperiod (hrs dark / hrs light): 12hrs dark / 12 hrs lightIN-LIFE DATES: From: To: no data

Test system

Vehicle:

unchanged (no vehicle)

Controls:

other: untreated eye served as control

Amount / concentration applied:

0.1 ml per animal

Duration of treatment / exposure:

1 administration into conjunctival sac of left eye

Observation period (in vivo):

21 days

Number of animals or in vitro replicates:

3

Details on study design:

SCORING SYSTEM: Corneal opacity, erythema, chemosis, secreta and iritis were classified according to the Draize score (Draize et al. 1944).

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: all

Score:

> 1 - < 42.8

Max. score:

110

Reversibility:

fully reversible within: 21 days

Irritant / corrosive response data:

II: moderate or severe irritantCorneal involvement or irritation that persists for more than 24 hrs but recovers within 21 days after treatment

Applicant's summary and conclusion

Interpretation of results:

other: moderate to severe irritant

Remarks:

Criteria used for interpretation of results: expert judgment

Conclusions:

Styrene oxide was classified as moderate to severe irritant.

Executive summary:

In a primary eye irritation study similar to OECD Guideline 405, 0.1 mL of 1,2-epoxyethylbenzene (styrene oxide) was instilled into the conjunctival sac of three Japanese White rabbits. Animals then were observed for 21 days. Irritation was scored by the method of Draize. Styrene oxide was classified as moderate to severe irritant as it provoked corneal involvement or irritation that persisted for more than 24 hours but recovers within 21 days after treatment.