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Diss Factsheets
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EC number: 203-570-0 | CAS number: 108-30-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://poisoncentres.echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- > 300 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- The EC50 of 3,5-dichlorophenol was in the accepted range of 5.1 to 30.3 mg/L. The actual values of the EC20, EC50 and EC80 for 3,5-dichlorophenol were: EC20 = 6.4 mg/L EC50 = 13.7 mg/L EC80 = 29.4 mg/L
- Reported statistics and error estimates:
- The concentrations of the test substance around 50 % inhibition (if such data exist) and the concentrations of the positive control are converted to log10. These values and the corresponding percentage of inhibition are subjected to linear regression analysis. These analyses are used to obtain the regression coefficients and the respective EC20, EC50 and EC80 values.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 3-hour EC50 and NOEC based on respiration inhibition were > 300 mg succinic acid/L.
- Executive summary:
Activated sludge obtained from a sewage treatment plant was exposed in a 3-hour respiration inhibition test to nominal concentrations of 0 (control), 7.7, 19.3, 48.2, 120.6 and 301.5 mg succinic acid/L. The test design included duplicate samples and followed the OECD 209 guideline (version 4 April 1984). Respiration rates of all samples with the test substance were in the range of 98.2–119.7 % of the control value. The 3-hour EC50 and NOEC based on respiration inhibition were therefore determined at > 300 mg succinic acid/L corresponding to 254 mg/L succinic acid.
This information is used in a read-across approach to assess the target substance succinic anhydride. For details and justification of read-across please refer to the attached report in section 13 of IUCLID.
Reference
Respiration rates and inhibition of samples
Sample |
Nominal Conc. (mg/L) |
mg O2/L at start |
mg O2/L at end |
Respiration rate (mg O2/L/h) |
% inhibition |
Control 1 |
0 |
6.39 |
4.61 |
16.90 |
6.11 |
Succinic acid |
7.7 |
7.41 |
5.23 |
17.68 |
1.79 |
19.3 |
7.29 |
5.04 |
18.05 |
-0.28 |
|
48.2 |
6.93 |
4.36 |
21.54 |
-19.66 |
|
120.6 |
6.74 |
3.97 |
21.53 |
-19.61 |
|
301.5 |
7.17 |
4.49 |
20.83 |
-15.73 |
|
Control 2 |
0 |
6.74 |
4.41 |
19.10 |
-6.11 |
|
5.1 |
7.21 |
4.99 |
16.82 |
6.56 |
12.3 |
8.21 |
7.27 |
8.25 |
54.19 |
|
30.3 |
8.57 |
8.07 |
4.14 |
76.98 |
Negative values indicate enhanced respiration compared to the control cultures.
Description of key information
The potential toxicity of succinic anhydride to aquatic microorganisms is assessed via read-across from its hydrolytic transformation product succinic acid.
Activated sludge obtained from a sewage treatment plant was exposed in a 3-hour respiration inhibition test to nominal concentrations of 0 (control), 7.7, 19.3, 48.2, 120.6 and 301.5 mg succinic acid/L. The test design included duplicate samples and followed OECD guideline 209. The 3-hour EC50 based on respiration inhibition was > 300 mg succinic acid/L. As succinic anhydride hydrolyses rapidly to succinic acid (DT50 = approx. 5 min), succinic anhydride is not expected to inhibit microbial respiration in STPs at concentrations of > 300 mg/L, corresponding to 254 mg/L succinic anhydride.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 300 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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