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EC number: 411-490-4 | CAS number: 7027-11-4 ISOPHORONENITRILE, IPN
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1991-03-06 to 1991-03-07
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions: No analytical monitoring, duration only 24 hours
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 991
- Report date:
- 1991
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-cyano-3,5,5-trimethylcyclohexanone
- EC Number:
- 411-490-4
- EC Name:
- 3-cyano-3,5,5-trimethylcyclohexanone
- Cas Number:
- 7027-11-4
- Molecular formula:
- C10H15NO
- IUPAC Name:
- 3-cyano-3,5,5-trimethylcyclohexanone
- Details on test material:
- Test substance: Isophorone nitrile of Degussa AG. Batch No. 64-II/78 I,90. Purity 99.6 % (GC).
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- - at 0 and 24 hours
Test solutions
- Vehicle:
- no
- Details on test solutions:
- STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Dispersion: Accurate weighing and dissolving
- Vehicle, solvent: Dilution water
- Concentration in vehicle/ solvent: 1000 mg/l
STABILITY OF THE TEST CHEMICAL SOLUTIONS: Visually clear solutions
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISMS
- Age: < 24 hours at study initiation
- Feeding during test: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
Test conditions
- Test temperature:
- - Test temperature: 19.9-20.3 °C
- pH:
- - pH: 8.0-8.1
- Dissolved oxygen:
- - Dissolved oxygen: 7.6-8.7 mg/l
- Nominal and measured concentrations:
- 100; 180; 320; 560; 1000 mg/l (nominal concentrations)
- Details on test conditions:
- DILUTION WATER
- Source: Groundwater from a locality near Linschoten (NL), several salts added
- Aeration: Only prior to test
- Hardness: 217 mg/l as CaCO3
- TOC: 1.5 mg total inorganic carbon/l
- Ca/Mg ratio: 1.43/0.74 [mmol/l]
- Na/K ratio: 1.33/0.24 [mmol/l]
- 2.79 mmol Cl-/l; 0.74 mmol SO4--/l
- pH: 8.0-8.2
TEST SYSTEM
- Exposure vessel type: 150 ml beaker with 100 ml test solution
- Number of replicates, individuals per replicate: 4 replicates with 5 individuals each
- Control group: dilution water
- Photoperiod: 16 h light / 8 h dark with 2 x 30 min transition period
TEST PARAMETER:
- Main: Immobility as defined in OECD Guideline 202. Additionally comparison of visually observable criteria (swimming behavior, color, others) with control, and microscopic check of all 1000 mg/l and half of the 560 mg/l daphnids.#
SAMPLING: At beginning and end (Temperature, dissolved oxygen, pH, test parameters)
STATISTICS
- EC50 values and their corresponding confidence intervals were calculated by means of a parametric model developed by Kooijman - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 320 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: mobility and condition
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 560 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 827 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 714-956 mg/L
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Other effects: 100-320 mg/l: condition similar to control animals; 560 mg/l: slow and irregular movements and lower positions of all mobile
daphnia, 1000 mg/l: 3 animals dead, 12 immobile, 5 with slow movements - Results with reference substance (positive control):
- - no data available
- Reported statistics and error estimates:
- - Concentration / response curve: Slope 0.17 (95 % confidence interval: 0.08-0.26)
Any other information on results incl. tables
RESULTS
- Effect data (Immobilization):
Control: 0/20
100 mg/l: 0/20
180 mg/l: 0/20
320 mg/l: 0/20
560 mg/l: 2/20
1000 mg/l: 15/20
Since 10 % immobilization in the controls is allowed by the test guideline, 560
mg/l with 2/20 immobile daphnia was considered as EC0 by the authors.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC50 (24 hours) was calculated to be 827 mg/L indicating that the test substance poses no significant hazard to Daphnia magna.
- Executive summary:
The acute toxictiy of isophorone nitrile on Daphnia magna was investigated over a period of 24 hours by TNO (1991) according to OECD 202. Five concentrations ranging from 100 to 1000 mg/L plus controls were tested. The following enpoints were derived:
EC50 (24 hours): 827 mg/L (CL: 714 -956 mg/L)
NOEC (24 hours): 320 mg/L
The study was assessed as "reliable with restrictions".
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